T-cell motility methods and protocols - Verma, Navin Kumar ca. 20./21. Jh (Springer New York, 2019)
An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility – Isolation of Human Peripheral Blood T Lymphocytes – A Laboratory Model to Study T-Cell Motility – Quantification of T-Cell Migratory Phenotypes using High Content Analysis – Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time – Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells – Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis – Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes – GapmeR-Mediated Gene Silencing in Motile T-Cells – Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy – Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes – Immunometabolomic Phenotyping of Motile T-Cells – Profiling Activity of Cellular Kinases in Migrating T-Cells – Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes – A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy – Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides – Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility – Computational Analysis of Protein-Protein Interactions in Motile T-Cells