Mouse oocyte development: methods and protocols
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1818 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation |
| Beschreibung: | Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse -- In vitro Culture of Mouse Oocytes for Meiotic Maturation -- In vitro Maturation and Culture of Human Oocytes -- Pronuclear Transfer in Human Oocytes -- In vitro Fertilization of Mouse Oocyte -- Profiling Maternal mRNA Translation during Oocyte Development -- Transcriptome Profiling of Single Mouse Oocytes -- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation -- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation -- Triple-colour Live Imaging of Mouse Oocytes -- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes -- Manipulating Cohesin Levels in Live Mouse Oocytes -- Optogenetic Manipulation of Mouse Oocytes -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension |
| Beschreibung: | 1 Online-Ressource (XI, 174 Seiten) Illustrationen |
| ISBN: | 9781493986033 |
| DOI: | 10.1007/978-1-4939-8603-3 |
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| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
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| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.1007/978-1-4939-8603-3 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:44:34Z |
| indexdate | 2024-07-10T09:17:36Z |
| institution | BVB |
| isbn | 9781493986033 |
| language | English |
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| spelling | Mouse oocyte development methods and protocols edited by Marie-Hélène Verlhac, Marie-Emilie Terret 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 174 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1818 Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse -- In vitro Culture of Mouse Oocytes for Meiotic Maturation -- In vitro Maturation and Culture of Human Oocytes -- Pronuclear Transfer in Human Oocytes -- In vitro Fertilization of Mouse Oocyte -- Profiling Maternal mRNA Translation during Oocyte Development -- Transcriptome Profiling of Single Mouse Oocytes -- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation -- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation -- Triple-colour Live Imaging of Mouse Oocytes -- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes -- Manipulating Cohesin Levels in Live Mouse Oocytes -- Optogenetic Manipulation of Mouse Oocytes -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation Cell biology Verlhac, Marie-Hélène (DE-588)1163684643 edt Terret, Marie-Emilie (DE-588)116368466X edt Erscheint auch als Druck-Ausgabe 9781493986026 Erscheint auch als Druck-Ausgabe 9781493986040 Erscheint auch als Druck-Ausgabe 9781493993406 https://doi.org/10.1007/978-1-4939-8603-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Mouse oocyte development methods and protocols Cell biology |
| title | Mouse oocyte development methods and protocols |
| title_auth | Mouse oocyte development methods and protocols |
| title_exact_search | Mouse oocyte development methods and protocols |
| title_exact_search_txtP | Mouse oocyte development methods and protocols |
| title_full | Mouse oocyte development methods and protocols edited by Marie-Hélène Verlhac, Marie-Emilie Terret |
| title_fullStr | Mouse oocyte development methods and protocols edited by Marie-Hélène Verlhac, Marie-Emilie Terret |
| title_full_unstemmed | Mouse oocyte development methods and protocols edited by Marie-Hélène Verlhac, Marie-Emilie Terret |
| title_short | Mouse oocyte development |
| title_sort | mouse oocyte development methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-8603-3 |
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