Epithelial cell culture: methods and protocols
This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroi...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1817 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab |
| Beschreibung: | Normal Human Thyrocytes in Culture -- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia -- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture -- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro -- Differentiation of gastrointestinal cell lines by culture in semi-wet interface -- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research -- Amniotic epithelial cell culture. Corresponding author -- Bovine granulosa cell culture -- Bioencapsulation of oocytes and granulosa cells -- Ovine granulosa cells isolation and culture to improve oocyte quality -- 3D model replicating the intestinal function to evaluate drug permeability -- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture -- Long-term culture of intestinal organoids -- Bovine mammary organoids: a model to study epithelial mammary cells -- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids -- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment -- Murine and human mammary cancer cell lines: functional tests -- In vitro porcine colon culture -- Primary cultures of olfactory neurons from the avian olfactory epithelium |
| Beschreibung: | 1 Online-Ressource (XIII, 214 Seiten) Illustrationen |
| ISBN: | 9781493986002 |
| DOI: | 10.1007/978-1-4939-8600-2 |
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Datensatz im Suchindex
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| dewey-full | 571.6 |
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| discipline | Biologie |
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| doi_str_mv | 10.1007/978-1-4939-8600-2 |
| edition | 1st ed. 2018 |
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| isbn | 9781493986002 |
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| spelling | Epithelial cell culture methods and protocols edited by Mario Baratta 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XIII, 214 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1817 Normal Human Thyrocytes in Culture -- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia -- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture -- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro -- Differentiation of gastrointestinal cell lines by culture in semi-wet interface -- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research -- Amniotic epithelial cell culture. Corresponding author -- Bovine granulosa cell culture -- Bioencapsulation of oocytes and granulosa cells -- Ovine granulosa cells isolation and culture to improve oocyte quality -- 3D model replicating the intestinal function to evaluate drug permeability -- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture -- Long-term culture of intestinal organoids -- Bovine mammary organoids: a model to study epithelial mammary cells -- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids -- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment -- Murine and human mammary cancer cell lines: functional tests -- In vitro porcine colon culture -- Primary cultures of olfactory neurons from the avian olfactory epithelium This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab Cell biology Zellkultur (DE-588)4067547-6 gnd rswk-swf Epithelzelle (DE-588)4259648-8 gnd rswk-swf Epithelzelle (DE-588)4259648-8 s Zellkultur (DE-588)4067547-6 s DE-604 Baratta, Mario edt Erscheint auch als Druck-Ausgabe 9781493985999 Erscheint auch als Druck-Ausgabe 9781493986019 Erscheint auch als Druck-Ausgabe 9781493993390 https://doi.org/10.1007/978-1-4939-8600-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Epithelial cell culture methods and protocols Cell biology Zellkultur (DE-588)4067547-6 gnd Epithelzelle (DE-588)4259648-8 gnd |
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| title | Epithelial cell culture methods and protocols |
| title_auth | Epithelial cell culture methods and protocols |
| title_exact_search | Epithelial cell culture methods and protocols |
| title_exact_search_txtP | Epithelial cell culture methods and protocols |
| title_full | Epithelial cell culture methods and protocols edited by Mario Baratta |
| title_fullStr | Epithelial cell culture methods and protocols edited by Mario Baratta |
| title_full_unstemmed | Epithelial cell culture methods and protocols edited by Mario Baratta |
| title_short | Epithelial cell culture |
| title_sort | epithelial cell culture methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology Zellkultur (DE-588)4067547-6 gnd Epithelzelle (DE-588)4259648-8 gnd |
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