Mitochondrial bioenergetics: Methods and Protocols
This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochon...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 2nd ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1782 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries |
| Beschreibung: | Overview of Mitochondrial Bioenergetics -- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells -- High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria -- High-Throughput Analysis of Mitochondrial Oxygen Consumption -- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices -- Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria -- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells -- Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production -- Measurement of Proton Leak in Isolated Mitochondria -- Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy -- - Mitochondrial Permeability Transition Pore and Calcium Handling -- Redox Equivalents and Mitochondrial Bioenergetics -- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis -- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective -- Monitoring the Mitochondrial Dynamics in Mammalian Cells -- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria -- Plate-Based Measurement of Respiration by Isolated Mitochondria -- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells -- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue -- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells -- Imaging of Mitochondrial pH Using SNARF-1 -- Relation between Mitochondrial Membrane Potential and ROS Formation -- - Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks -- Measurement of Mitochondrial ROS Formation |
| Beschreibung: | 1 Online-Ressource (XI, 422 Seiten) Illustrationen |
| ISBN: | 9781493978311 |
| DOI: | 10.1007/978-1-4939-7831-1 |
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| 500 | |a - Mitochondrial Permeability Transition Pore and Calcium Handling -- Redox Equivalents and Mitochondrial Bioenergetics -- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis -- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective -- Monitoring the Mitochondrial Dynamics in Mammalian Cells -- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria -- Plate-Based Measurement of Respiration by Isolated Mitochondria -- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells -- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue -- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells -- Imaging of Mitochondrial pH Using SNARF-1 -- Relation between Mitochondrial Membrane Potential and ROS Formation -- | ||
| 500 | |a - Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks -- Measurement of Mitochondrial ROS Formation | ||
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| doi_str_mv | 10.1007/978-1-4939-7831-1 |
| edition | 2nd ed. 2018 |
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| isbn | 9781493978311 |
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| spelling | Mitochondrial bioenergetics Methods and Protocols edited by Carlos M. Palmeira, António J. Moreno 2nd ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 422 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1782 Overview of Mitochondrial Bioenergetics -- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells -- High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria -- High-Throughput Analysis of Mitochondrial Oxygen Consumption -- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices -- Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria -- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells -- Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production -- Measurement of Proton Leak in Isolated Mitochondria -- Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy -- - Mitochondrial Permeability Transition Pore and Calcium Handling -- Redox Equivalents and Mitochondrial Bioenergetics -- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis -- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective -- Monitoring the Mitochondrial Dynamics in Mammalian Cells -- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria -- Plate-Based Measurement of Respiration by Isolated Mitochondria -- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells -- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue -- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells -- Imaging of Mitochondrial pH Using SNARF-1 -- Relation between Mitochondrial Membrane Potential and ROS Formation -- - Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks -- Measurement of Mitochondrial ROS Formation This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries Cell biology Palmeira, Carlos Manuel Marques (DE-588)123636676X edt Moreno, António J. edt Erscheint auch als Druck-Ausgabe 9781493978304 Erscheint auch als Druck-Ausgabe 9781493978328 Erscheint auch als Druck-Ausgabe 9781493992980 https://doi.org/10.1007/978-1-4939-7831-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Mitochondrial bioenergetics Methods and Protocols Cell biology |
| title | Mitochondrial bioenergetics Methods and Protocols |
| title_auth | Mitochondrial bioenergetics Methods and Protocols |
| title_exact_search | Mitochondrial bioenergetics Methods and Protocols |
| title_exact_search_txtP | Mitochondrial bioenergetics Methods and Protocols |
| title_full | Mitochondrial bioenergetics Methods and Protocols edited by Carlos M. Palmeira, António J. Moreno |
| title_fullStr | Mitochondrial bioenergetics Methods and Protocols edited by Carlos M. Palmeira, António J. Moreno |
| title_full_unstemmed | Mitochondrial bioenergetics Methods and Protocols edited by Carlos M. Palmeira, António J. Moreno |
| title_short | Mitochondrial bioenergetics |
| title_sort | mitochondrial bioenergetics methods and protocols |
| title_sub | Methods and Protocols |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7831-1 |
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