Schizosaccharomyces pombe: methods and protocols
This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materi...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1721 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community |
| Beschreibung: | Preparation of Solutions and Reagents -- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing -- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe -- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs -- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast -- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast -- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis -- The No-nonsens SDS-PAGE -- Crystallization of Recombinant α-actinin and Related Proteins -- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy -- Antibody Pull-down Experiments in Fission Yeast -- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation -- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo -- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe -- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast -- Random Spore Analysis in Fission Yeast -- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains -- Schizosaccharomyces pombe Biotechnological Applications in Winemaking -- Schizosaccharomyces pombe Isolation Protocol. |
| Beschreibung: | 1 Online-Ressource (XII, 244 Seiten) Illustrationen |
| ISBN: | 9781493975464 |
| DOI: | 10.1007/978-1-4939-7546-4 |
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| spelling | Schizosaccharomyces pombe methods and protocols edited by Teresa L. Singleton 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XII, 244 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1721 Preparation of Solutions and Reagents -- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing -- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe -- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs -- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast -- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast -- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis -- The No-nonsens SDS-PAGE -- Crystallization of Recombinant α-actinin and Related Proteins -- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy -- Antibody Pull-down Experiments in Fission Yeast -- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation -- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo -- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe -- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast -- Random Spore Analysis in Fission Yeast -- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains -- Schizosaccharomyces pombe Biotechnological Applications in Winemaking -- Schizosaccharomyces pombe Isolation Protocol. This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community Microbiology Schizosaccharomyces pombe (DE-588)4179645-7 gnd rswk-swf Schizosaccharomyces pombe (DE-588)4179645-7 s DE-604 Singleton, Teresa L. (DE-588)1153802317 edt Erscheint auch als Druck-Ausgabe 9781493975457 Erscheint auch als Druck-Ausgabe 9781493975471 Erscheint auch als Druck-Ausgabe 9781493985166 https://doi.org/10.1007/978-1-4939-7546-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Schizosaccharomyces pombe methods and protocols Microbiology Schizosaccharomyces pombe (DE-588)4179645-7 gnd |
| subject_GND | (DE-588)4179645-7 |
| title | Schizosaccharomyces pombe methods and protocols |
| title_auth | Schizosaccharomyces pombe methods and protocols |
| title_exact_search | Schizosaccharomyces pombe methods and protocols |
| title_exact_search_txtP | Schizosaccharomyces pombe methods and protocols |
| title_full | Schizosaccharomyces pombe methods and protocols edited by Teresa L. Singleton |
| title_fullStr | Schizosaccharomyces pombe methods and protocols edited by Teresa L. Singleton |
| title_full_unstemmed | Schizosaccharomyces pombe methods and protocols edited by Teresa L. Singleton |
| title_short | Schizosaccharomyces pombe |
| title_sort | schizosaccharomyces pombe methods and protocols |
| title_sub | methods and protocols |
| topic | Microbiology Schizosaccharomyces pombe (DE-588)4179645-7 gnd |
| topic_facet | Microbiology Schizosaccharomyces pombe |
| url | https://doi.org/10.1007/978-1-4939-7546-4 |
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