Host-pathogen interactions methods and protocols - Medina, Carlos; López-Baena, Francisco Javier (Springer New York, 2018)
Genetic Association Studies in Host-Pathogens Interaction Analysis.- Bacterial Genotyping Methods: From the Basics to Modern -- Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens -- The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection.- lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens -- Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity -- Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage -- Organoids as a Model to Study Infectious Disease -- Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins -- Systems Biology Modeling to Study Pathogen-Host Interactions -- Phage Therapy: Various Perspectives on How to Improve the Art --
- Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression -- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth -- Detection of Bacterial Quorum Sensing Molecules -- Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae -- Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation -- In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors -- Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains -- Methods to Quantify Biotic-Induced Stress in Plants -- From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy --
- Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria -- Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria -- A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean -- Methods for the Characterization of Plant-Growth Promoting Rhizobacteria