Synthetic biology methods and protocols - Braman, Jeffrey ca. 20./21. Jh (Springer New York, 2018)
Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria -- Negative Regulation Gene Circuits for Efflux Pump Control -- Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen -- Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits -- Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola -- Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux -- Construction of CRISPR Libraries for Functional Screening -- Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering -- Dynamic Management of Codon Compression for Saturation Mutagenesis -- Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9 --
- Combinatorial Evolution of DNA with RECODE -- Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles -- Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1 -- Programming Biomaterial Interactions Using Engineered Living Cells -- Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems -- Production of a Human Cell Line with a Plant Chromosome -- Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example -- CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica -- Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells -- Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs -- Engineering Genomes with Genotype Specification Language -- Automated Visualization of Genetic Designs Using DNAplotlib -- DNA-Based Analog Computing --
- DNA Assembly with the DATEL Method -- EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology -- Designing and Assembling Plasmids for Construction of Escherichia coli Biosensor for Vibrio cholerae Detection -- A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors -- Standardized Cloning and Curing of Plasmids -- STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly