CRISPR-Cas methods: [Volume 1]
This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediate...
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2020]
|
| Schriftenreihe: | Springer protocols
Springer protocols |
| Schlagworte: | |
| Zusammenfassung: | This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism |
| Beschreibung: | 1. Efficient Homologous Recombination Mediated in Planta Gene Targeting by Egg-cell Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis; 2. Rice gene knockout or down regulation through CRISPR-Cas93. Simultaneous CRISPR/Cas-mediated Single Base Editing in Rice Plant at More than One Locus 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora6. An effective CRISPR/Cas9 technology for efficiently isolating positive transformants and transgene-free mutants in a wide range of plant species7. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley8. Genome editing of mammalian cells using CRISPR/Cas: From in silico designing to in-culture validation9. Cloning free (DNA-free) CRISPR/Cas9-mediated gene knockout in human liver cell line and its detection10. A procedure to design guide RNA, assembly fragments, and detect mutation for genome editing in flax 11. Delivery of Cas9/sgRNA RNP into wheat microspores using synthetic CPP for genome editing and gene expression modulation12. CRISPR- Cas9 mediated gene editing in wheat: a step by step protocol13. CRISPR-Cas9 based genome editing of banana14. Homology-directed transgene-free gene editing in Chlamydomonas reinhardtii |
| Beschreibung: | xiv, 278 Seiten Illustrationen |
| ISBN: | 9781071606155 |
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| 500 | |a 1. Efficient Homologous Recombination Mediated in Planta Gene Targeting by Egg-cell Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis; 2. Rice gene knockout or down regulation through CRISPR-Cas93. Simultaneous CRISPR/Cas-mediated Single Base Editing in Rice Plant at More than One Locus 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora6. An effective CRISPR/Cas9 technology for efficiently isolating positive transformants and transgene-free mutants in a wide range of plant species7. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley8. Genome editing of mammalian cells using CRISPR/Cas: From in silico designing to in-culture validation9. Cloning free (DNA-free) CRISPR/Cas9-mediated gene knockout in human liver cell line and its detection10. A procedure to design guide RNA, assembly fragments, and detect mutation for genome editing in flax 11. Delivery of Cas9/sgRNA RNP into wheat microspores using synthetic CPP for genome editing and gene expression modulation12. CRISPR- Cas9 mediated gene editing in wheat: a step by step protocol13. CRISPR-Cas9 based genome editing of banana14. Homology-directed transgene-free gene editing in Chlamydomonas reinhardtii | ||
| 520 | |a This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism | ||
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| index_date | 2024-07-03T18:38:27Z |
| indexdate | 2024-07-10T09:16:01Z |
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| spelling | CRISPR-Cas methods [Volume 1] edited by M. Tofazzal Islam New York, NY Humana Press [2020] xiv, 278 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Springer protocols 1. Efficient Homologous Recombination Mediated in Planta Gene Targeting by Egg-cell Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis; 2. Rice gene knockout or down regulation through CRISPR-Cas93. Simultaneous CRISPR/Cas-mediated Single Base Editing in Rice Plant at More than One Locus 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora6. An effective CRISPR/Cas9 technology for efficiently isolating positive transformants and transgene-free mutants in a wide range of plant species7. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley8. Genome editing of mammalian cells using CRISPR/Cas: From in silico designing to in-culture validation9. Cloning free (DNA-free) CRISPR/Cas9-mediated gene knockout in human liver cell line and its detection10. A procedure to design guide RNA, assembly fragments, and detect mutation for genome editing in flax 11. Delivery of Cas9/sgRNA RNP into wheat microspores using synthetic CPP for genome editing and gene expression modulation12. CRISPR- Cas9 mediated gene editing in wheat: a step by step protocol13. CRISPR-Cas9 based genome editing of banana14. Homology-directed transgene-free gene editing in Chlamydomonas reinhardtii This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism (DE-588)4143413-4 Aufsatzsammlung gnd-content Islam, M. Tofazzal 1966- (DE-588)1193274850 edt Bhowmik, Pankaj K. edt Molla, Kutubuddin Ali edt (DE-604)BV047606357 1 Erscheint auch als Online-Ausgabe 978-1-0716-0616-2 |
| spellingShingle | CRISPR-Cas methods |
| subject_GND | (DE-588)4143413-4 |
| title | CRISPR-Cas methods |
| title_auth | CRISPR-Cas methods |
| title_exact_search | CRISPR-Cas methods |
| title_exact_search_txtP | CRISPR-Cas methods |
| title_full | CRISPR-Cas methods [Volume 1] edited by M. Tofazzal Islam |
| title_fullStr | CRISPR-Cas methods [Volume 1] edited by M. Tofazzal Islam |
| title_full_unstemmed | CRISPR-Cas methods [Volume 1] edited by M. Tofazzal Islam |
| title_short | CRISPR-Cas methods |
| title_sort | crispr cas methods |
| topic_facet | Aufsatzsammlung |
| volume_link | (DE-604)BV047606357 |
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