The (patho)physiology of ion channels in human sperm:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Münster
[2021]
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| Schlagworte: | |
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| Beschreibung: | v, 90 Seiten Illustrationen 30 cm |
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| 245 | 1 | 0 | |a The (patho)physiology of ion channels in human sperm |c vorgelegt von Samuel Zachary Young |
| 264 | 1 | |a Münster |c [2021] | |
| 300 | |a v, 90 Seiten |b Illustrationen |c 30 cm | ||
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Datensatz im Suchindex
| _version_ | 1804182731931779072 |
|---|---|
| adam_text | I
TABLE
OF
CONTENTS
I
TABLE
OF
CONTENTS
......................................................................................................
I
II
ABBREVIATIONS
.........................................................................................................
III
1
INTRODUCTION
.............................................................................................................
1
1.1
PHYSIOLOGY
OF
HUMAN
SPERM
......................................................................................
1
1.2
A
SPERM
S
JOURNEY
THROUGH
THE
FEMALE
REPRODUCTIVE
TRACT
.........................................
1
1.3
SPERM
MATURATION,
NAVIGATION,
AND
PENETRATION
OF
THE
EGG
COAT
..............................
3
1.4
CONTROL
OF
SPERM
BEHAVIOR
.........................................................................................
6
1.5
ORGANIZATION,
STRUCTURE,
AND
FUNCTION
OF
CATSPER
.....................................................
7
1.6
CATSPER
IS
ESSENTIAL
FOR
SPERM
FUNCTION
AND
FERTILIZATION
.........................................
12
1.7
MALE
INFERTILITY
AND
ASSISTED
REPRODUCTIVE
TECHNOLOGY
(ART)
...................................
15
1.8
AIM
OF
THE
THESIS
......................................................................................................
16
2
MATERIALS
AND
METHODS
..........................................................................................
17
2.1
CHEMICALS
................................................................................................................
17
2.2
BUFFER
COMPOSITIONS
AND
PREPARATION
......................................................................
17
2.3
PREPARATION
OF
HUMAN
SPERM
..................................................................................
17
2.4
VIDEO
MICROSCOPY
....................................................................................................
19
2.5
MEASUREMENT
OF
INTRACELLULAR
CAMP
CONTENT
..........................................................
23
2.6
FLUORESCENCE
MEASUREMENTS
OF
SPERM
POPULATIONS
..............................................
25
2.7
GENETIC
ANALYSIS
.....................................................................................................
28
2.8
ELECTROPHYSIOLOGY
....................................................................................................
29
2.9
DATA
ANALYSIS
..........................................................................................................
30
3
RESULTS
...................................................................................................................
32
3.1
DEVELOPMENT
OF
A
FUNCTIONAL
ASSAY
TO
ASSESS
CATSPER
ACTIVITY
IN
SPERM
..............
32
3.2
MOLECULAR
MECHANISM(S)
UNDERLYING
THE
MOTILITY
DECAY
IN
CA
2+
-FREE
HTF
.............
34
3.3
IMPLEMENTATION
OF
THE
CATSPER-ACTIVITY-TEST
INTO
ROUTINE
ANDROLOGICAL
(IN)FERTILITY
WORKUP
AND
IDENTIFICATION
OF
CATSPER-DEFICIENT
PATIENTS
...................................................44
3.4
VALIDATION
OF
THE
LOSS
OF
CATSPER
FUNCTION
AND
CLINICAL
AS
WELL
AS
GENETIC
EVALUATION
OF
CATSPER-DEFICIENT
PATIENTS
IDENTIFIED
BY
THE
CAT
.........................................................
56
3.5
CHARACTERIZATION
OF
THE
MOTILITY
PHENOTYPE
OF
CATSPER-DEFICIENT
SPERM
FROM
CATSPER2
7
-
PATIENTS
......................................................................................................
61
4
DISCUSSION
...........................................................................................................
75
4.1
THE
CATSPER-ACTIVITY-TEST:
HOW
DOES
THE
CATSPER
STATUS
OF
SPERM
TRANSLATE
INTO
A
MOTILITY
DECAY
IN
CA
2+
-FREE
HTF?
.....................................................................................
75
4.2
CATSPER-DEFICIENT
SPERM:
A
POWERFUL
TOOL
TO
INVESTIGATE
THE
ROLE
OF
CATSPER
FUNCTION
IN
SPERM
PHYSIOLOGY
AND
BEHAVIOR
...................................................................................
77
4.3
IDENTIFICATION
OF
CATSPER-DEFICIENT
PATIENTS:
CLINICAL
RELEVANCE
..............................
86
4.4
THE
CAT:
ROADMAP
FOR
BECOMING
A
COMMERCIALLY
AVAILABLE
IN
VITRO
DIAGNOSTIC
MEDICAL
DEVICE
..................................................................................................................
88
4.5 THE
CAT
:
BLUEPRINT
FOR
DEVELOPING
OTHER
FUNCTIONAL
FERTILITY
ASSAYS
.......................
90
5
REFERENCES
............................................................................................................
91
6
SUPPLEMENTARY
MATERIALS
...................................................................................
105
CURRICULUM
VITAE
.......................................................................................................
106
SCIENTIFIC
CONTRIBUTIONS
...........................................................................................
107
ACKNOWLEDGEMENTS
..................................................................................................
109
II
|
| adam_txt |
I
TABLE
OF
CONTENTS
I
TABLE
OF
CONTENTS
.
I
II
ABBREVIATIONS
.
III
1
INTRODUCTION
.
1
1.1
PHYSIOLOGY
OF
HUMAN
SPERM
.
1
1.2
A
SPERM
'
S
JOURNEY
THROUGH
THE
FEMALE
REPRODUCTIVE
TRACT
.
1
1.3
SPERM
MATURATION,
NAVIGATION,
AND
PENETRATION
OF
THE
EGG
COAT
.
3
1.4
CONTROL
OF
SPERM
BEHAVIOR
.
6
1.5
ORGANIZATION,
STRUCTURE,
AND
FUNCTION
OF
CATSPER
.
7
1.6
CATSPER
IS
ESSENTIAL
FOR
SPERM
FUNCTION
AND
FERTILIZATION
.
12
1.7
MALE
INFERTILITY
AND
ASSISTED
REPRODUCTIVE
TECHNOLOGY
(ART)
.
15
1.8
AIM
OF
THE
THESIS
.
16
2
MATERIALS
AND
METHODS
.
17
2.1
CHEMICALS
.
17
2.2
BUFFER
COMPOSITIONS
AND
PREPARATION
.
17
2.3
PREPARATION
OF
HUMAN
SPERM
.
17
2.4
VIDEO
MICROSCOPY
.
19
2.5
MEASUREMENT
OF
INTRACELLULAR
CAMP
CONTENT
.
23
2.6
FLUORESCENCE
MEASUREMENTS
OF
SPERM
POPULATIONS
.
25
2.7
GENETIC
ANALYSIS
.
28
2.8
ELECTROPHYSIOLOGY
.
29
2.9
DATA
ANALYSIS
.
30
3
RESULTS
.
32
3.1
DEVELOPMENT
OF
A
FUNCTIONAL
ASSAY
TO
ASSESS
CATSPER
ACTIVITY
IN
SPERM
.
32
3.2
MOLECULAR
MECHANISM(S)
UNDERLYING
THE
MOTILITY
DECAY
IN
CA
2+
-FREE
HTF
.
34
3.3
IMPLEMENTATION
OF
THE
CATSPER-ACTIVITY-TEST
INTO
ROUTINE
ANDROLOGICAL
(IN)FERTILITY
WORKUP
AND
IDENTIFICATION
OF
CATSPER-DEFICIENT
PATIENTS
.44
3.4
VALIDATION
OF
THE
LOSS
OF
CATSPER
FUNCTION
AND
CLINICAL
AS
WELL
AS
GENETIC
EVALUATION
OF
CATSPER-DEFICIENT
PATIENTS
IDENTIFIED
BY
THE
CAT
.
56
3.5
CHARACTERIZATION
OF
THE
MOTILITY
PHENOTYPE
OF
CATSPER-DEFICIENT
SPERM
FROM
CATSPER2'
7
-
PATIENTS
.
61
4
DISCUSSION
.
75
4.1
THE
CATSPER-ACTIVITY-TEST:
HOW
DOES
THE
CATSPER
STATUS
OF
SPERM
TRANSLATE
INTO
A
MOTILITY
DECAY
IN
CA
2+
-FREE
HTF?
.
75
4.2
CATSPER-DEFICIENT
SPERM:
A
POWERFUL
TOOL
TO
INVESTIGATE
THE
ROLE
OF
CATSPER
FUNCTION
IN
SPERM
PHYSIOLOGY
AND
BEHAVIOR
.
77
4.3
IDENTIFICATION
OF
CATSPER-DEFICIENT
PATIENTS:
CLINICAL
RELEVANCE
.
86
4.4
THE
CAT:
ROADMAP
FOR
BECOMING
A
COMMERCIALLY
AVAILABLE
IN
VITRO
DIAGNOSTIC
MEDICAL
DEVICE
.
88
4.5 THE
CAT
:
BLUEPRINT
FOR
DEVELOPING
OTHER
FUNCTIONAL
FERTILITY
ASSAYS
.
90
5
REFERENCES
.
91
6
SUPPLEMENTARY
MATERIALS
.
105
CURRICULUM
VITAE
.
106
SCIENTIFIC
CONTRIBUTIONS
.
107
ACKNOWLEDGEMENTS
.
109
II |
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| institution | BVB |
| language | English |
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| spelling | Young, Samuel 1984- Verfasser (DE-588)1235270343 aut The (patho)physiology of ion channels in human sperm vorgelegt von Samuel Zachary Young Münster [2021] v, 90 Seiten Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universität Münster 2021 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1236155874/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032842262&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p aepkn 0,83581 20210713 DE-101 https://d-nb.info/provenance/plan#aepkn 2\p dnb 20210722 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Young, Samuel 1984- The (patho)physiology of ion channels in human sperm |
| subject_GND | (DE-588)4113937-9 |
| title | The (patho)physiology of ion channels in human sperm |
| title_auth | The (patho)physiology of ion channels in human sperm |
| title_exact_search | The (patho)physiology of ion channels in human sperm |
| title_exact_search_txtP | The (patho)physiology of ion channels in human sperm |
| title_full | The (patho)physiology of ion channels in human sperm vorgelegt von Samuel Zachary Young |
| title_fullStr | The (patho)physiology of ion channels in human sperm vorgelegt von Samuel Zachary Young |
| title_full_unstemmed | The (patho)physiology of ion channels in human sperm vorgelegt von Samuel Zachary Young |
| title_short | The (patho)physiology of ion channels in human sperm |
| title_sort | the patho physiology of ion channels in human sperm |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1236155874/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032842262&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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