Laboratory exercises in microbiology:
Gespeichert in:
Hauptverfasser: | , , , |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
Hoboken, NJ
Wiley
[2018]
|
Ausgabe: | Fifth edition |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | xv, 284, PA-16 Seiten Illustrationen |
ISBN: | 9781119462668 9781119462644 |
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MARC
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Datensatz im Suchindex
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adam_text | Contents Preface PART 1 ІІІ Introduction: Laboratory Operations and Safety xiii Basic Rules for Microbiology Laboratory Safety XV General Microscopy and Aseptic Technique 3 Laboratory Safety: Introduction to the Microscope Laboratory Safety and Procedures, Laboratory Procedures, 4 Simple Stain Technique, The Microscope, 4 5 6 Microscope Components (Plate 1), 7 Eukaryotic Versus Prokaryotic Cells (Plates 2A, 2B), Laboratory Cleanup, Microscope Cleanup, Discards, 10 11 General Cleanup, 11 Speaking of Safety, 2 10 10 11 Transfer and Isolation Techniques, Microbes in the Environment Transfer Technique, 17 Tube-to-Tube Transfers, 18 Basic Aseptic Technique Procedures, 18 Isolation Techniques: Streak Plate and Pour Plate (Optional), Streak Plate (Plate 3), 22 22 Prepare the Following Streak Plates Using the Cultures Provided, To Lap or Not to Lap—That is the Question, 25 Pour Plate (Plate 4) Optional, May be Done as a Demonstration, Microbes in the Environment, 26 Human Environment: Procedure, 27 25 25 17
vi CONTENTS Classroom/School Environment: Procedure, Incubation, Results, 27 27 27 Laboratory Cleanup, Discards, 28 28 General Cleanup, 28 PART 2 Microbial Morphology, Differential Stains 33 Cultural and Cellular Morphology Cultural Characteristics of Bacteria, Procedure: Plates (Plate 5), Procedure: Agar Slants, 34 35 Procedure: Broth (Plate 6), 35 Microbial Cellular Morphology, Smear Preparation, 36 Broth Preparation, 37 Agar Slant and Plate Preparation, Simple Stain Procedure, 33 35 37 37 Results from the Simple Stain Procedure (Plates 7-16), Results from Prepared Slide, Laboratory Cleanup, 38 38 38 43 Bacterial Growth Lactors Needed for Bacterial Growth, Measuring Bacterial Growth, 44 45 Procedure for Preparation of Spread Plate, 45 Procedure for Demonstration of a Bacterial Growth Curve, 47 53 Gram Stain and Acid-Fast Stain The Gram Stain, 53 The Gram Stain Technique (Traditional Method) (Plate 17A, B, C), Gram Stain: (Alternative Method), 55 The Acid-Last Stain Technique (Plate 18), Acid-Last Technique, Laboratory Cleanup, Incubation, 58 58 56 56 54
Contents Discards, 58 General Cleanup, 58 Slides and Microscopes, 58 Endospore Stain, Capsule Stain, and the Hanging Drop Technique The Endospore Stain, 62 63 The Capsule Stain, 63 The Capsule Stain Technique (Plate 21), The Hanging Drop Technique, Results, 61 61 The Spore Stain Technique (Plates 19, 20), Results, VÍÍ 63 64 64 69 The Fungi, 69 Results, 72 77 Viruses—Visualization and Enumeration Viruses, 77 Bacteriophage Enumeration, Results, 78 80 85 Parasitology PART 3 Microbial Control and Biochemistry 10 97 Microbial Sensitivity Testing Part 1 : Physical Methods Ultraviolet Light Sensitivity, Sterilization by the Use of UV Light, 98 98 The Penetrating Power of UV Light (Plate 34), 99 Heat Sensitivity (may be done as an alternative to the UV light procedure), Effect of Cold Temperature and Slow Freezing (Optional), Part 2: Chemical Methods Chemical Sensitivity, 102 Chemotherapeutic Agent Testing: The Kirby-Bauer Plate, The Kirby-Bauer Technique, Results, 107 104 102 103 101
viii CONTENTS 11 113 Bacterial Biochemistry Carbohydrate Metabolizing Enzymes, Results, 114 115 Carbohydrate Fermentation (Phenol Red Broth) Tubes (Plate 37), Amino Acid and Nitrogen Metabolism, Results, 115 116 117 Decarboxylase Tubes (Plate 42), 117 123 Gas Requirements of Microorganisms Growth of Anaerobes (may be done as a demonstration), Demonstration of Catalase, 124 125 Specialized Media Examples, 129 130 Inoculation of Blood Agar Plates for Demonstration of Alpha Hemolysis and Transport Medium, 131 Inoculation of Blood, Phenylethyl Alcohol, Mannitol Salt, Macconkey, and Eosin-Methylene Blue Agar Plates, 132 Results, 133 Inoculation of Triple Sugar bon Agar and Sulfide-Indole Motility Medium, Results, 134 Results of Transport Medium, PART 4 133 134 Medical Microbiology Genetics Ames Test, 143 144 UV Light Procedure, 145 Kirby-Bauer Procedure, 145 Results: Ames Test (Plate 55), 145 Results: UV Light Procedure (Plate 35), Results: Kirby-Bauer Test (Plates 36, 55), 146 146 149 Epidemiology Handwashing Procedure (Done with Three Students), Results: Handwashing (Plate 56), 151 150
Contents Fomite and Direct Transmission of Microbes, 151 Results: Fomite and Direct Transmission, 16 152 Results: Airborne and Cough/Sneeze Plates, 153 153 Results: Microbes in Makeup, 153 Specimen-Handling Protocols Quantitative Urinalysis, 159 160 Results: Urine Samples, 162 Specimens from the Gastrointestinal Tract, Results: GI Tract Isolation Technique, 17 152 Airborne Infections: Cough and Sneeze Plates, Microbes in Makeup (Optional), 163 163 Specific Laboratory Tests Catalase Test, 169 171 Alternative Method (to be done at the end of this exercise), Results: Catalase Test (Plate 46), 171 Results: Bacitracin Sensitivity (Plate 57), Coagulase Test, 172 172 Rapid Slide Test, 172 172 Results: Coagulase Tube Test (Plate 60), Novobiocin Sensitivity, Camp Test (Plate 58), Results: Camp, 172 172 Results: Novobiocin Sensitivity, 18 172 172 Results: Oxidase Test (Plate 59), Tube Test, 171 171 Bacitracin Sensitivity—Demonstration, Oxidase Test, 173 173 173 179 Serology Elisa Test: Test for Toxins A and В from Clostridium Difficile, Results, 182 Rapid Identification of Group A Antigen, Results, 182 184 Differentiation of Streptococci Using Latex Agglutination, Slidex Strepto-Kit®, Results, ¡X 184 186 Procedure Controls, 186 184 180
x CONTENTS Identification of a Bacterial Unknown Si 191 I Identification of Enteric Pathogens: Traditional Methods Results, 194 IMViC Test (See Plate 61), 194 Results of Gelatin Hydrolysis, 194 Results of the Oxidase Test (See Plate 59), 194 identification of Enteric Pathogens: Rapid Identification Methods EnteroPluri Test (Plate 62), 201 Compartment 1: Glucose Fermentation and Gas Production, 201 Compartments 2 and 3: Lysine and Ornithine Decarboxylation, Compartment 4: Sulfide and Indole Production, 202 202 Compartments 5-8: Adonitol, Lactose, Arabinose, and Sorbitol Fermentation, Compartment 9: Voges-Proskauer, 202 Compartment 10: Phenylalanine Deaminase and Dulcitol, Compartment 11 : Urea, 202 202 202 Compartment 12: Citrate, 202 Api® 20 E System (Plate 63), 202 Microtube 1 : Ortho-Nitrophenyl-Beta-D-Ortho-Nitrophenyl-Beta-D֊Galactopyranoside (Onpg), 203 Microtubes 2-4: Arginine Dihydrolase (Decarboxylase), Lysine Decarboxylase, And Ornithine Decarboxylase (Adh, Ldc, Ode), 203 Microtube 5: Citrate (Cit), 203 Microtube 6: Sulfide (H2s), Microtube 7: Urea (Ure), 203 203 Microtube 8: Tryptophan Deaminase (Tda), Microtube 9: Indole (Ind), 203 203 Microtube 10: Voges-Proskauer (Vp), Microtube 11 : Gelatin Hydrolysis (Gel), 203 203 Microtubes 12-20: Utilization of the Carbohydrates Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Saccharose (Sucrose), Melibiose, Amygdalin, and Arabinose (Glu, Man, Ino, Sor, Rha, Sac, Mel, Amy, And Ara), 203 Inoculation of Rapid Identification Media, Inoculation of EnteroPluri Test, 204 Inoculation of the Api 20 E System, Results, 205 204 204
201
Contents xi Identification of a Bacterial Unknown: The Gram-Negative Unknown Review, 209 209 Comparison Chart, Flowchart, 210 210 Computer Identification, 210 ID Value Worksheet for EnteroPluri Test II Results, 212 213 Identification of a Bacterial Unknown: The Gram-Positive Cocci 217 PART 6 Food and Environmental Microbiology identification and Quantitation of Microbial Numbers in a Water Sample 225 Presumptive Test: Analysis of a Contaminated Water Sample for the Presence of Coliforms, 226 Results, 227 Quantitation of Microbial Number in a Water Sample, Results (Plate 64), 24 227 228 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial Numbers Microbial Presence in a Food Product and Colony Counts, Results, 232 233 Recognition of Organism Genera, 233 237 Soil Microbiology Part 1 : The Recovery of Microorganisms from Soil, Results, 238 239 Isolation Of Soil Bacteria, Results, 239 239 Part 2: Isolation of Soil Bacteriophages of Bacillus Subtilis, Results, 231 240 239
xii CONTENTS ДДДЗ Microbial Ecology 245 Competition Between Bacteria, Results, 246 Bacteriocin Production, Results, 246 246 247 Bacterial-Fungal Interaction (Plate 65), Results, 247 247 251 Biofilms Growth of Dental Plaque in situ, Results, Soil Biofilm, Results, 252 253 253 253 Growth of Bacterial Biofilms in vitro, Results, 253 254 Bibliography 261 Answer Key for the Exercise Pre-Tests 263 Appendix 1 Flow Charts and Comparison Charts for the Identification of Enteric Gram Negative Rods Using Traditional Testing Procedures 265 Appendix 2 Flow Chart for the Identification of Enterobacteriaceae based on the API 20 E Rapid Identification System 269 Appendix 3 Gram-Positive Flow Chart 275 Photo Credit List 277 Index 279 Photographic Atlas PA-1
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adam_txt |
Contents Preface PART 1 ІІІ Introduction: Laboratory Operations and Safety xiii Basic Rules for Microbiology Laboratory Safety XV General Microscopy and Aseptic Technique 3 Laboratory Safety: Introduction to the Microscope Laboratory Safety and Procedures, Laboratory Procedures, 4 Simple Stain Technique, The Microscope, 4 5 6 Microscope Components (Plate 1), 7 Eukaryotic Versus Prokaryotic Cells (Plates 2A, 2B), Laboratory Cleanup, Microscope Cleanup, Discards, 10 11 General Cleanup, 11 Speaking of Safety, 2 10 10 11 Transfer and Isolation Techniques, Microbes in the Environment Transfer Technique, 17 Tube-to-Tube Transfers, 18 Basic Aseptic Technique Procedures, 18 Isolation Techniques: Streak Plate and Pour Plate (Optional), Streak Plate (Plate 3), 22 22 Prepare the Following Streak Plates Using the Cultures Provided, To Lap or Not to Lap—That is the Question, 25 Pour Plate (Plate 4) Optional, May be Done as a Demonstration, Microbes in the Environment, 26 Human Environment: Procedure, 27 25 25 17
vi CONTENTS Classroom/School Environment: Procedure, Incubation, Results, 27 27 27 Laboratory Cleanup, Discards, 28 28 General Cleanup, 28 PART 2 Microbial Morphology, Differential Stains 33 Cultural and Cellular Morphology Cultural Characteristics of Bacteria, Procedure: Plates (Plate 5), Procedure: Agar Slants, 34 35 Procedure: Broth (Plate 6), 35 Microbial Cellular Morphology, Smear Preparation, 36 Broth Preparation, 37 Agar Slant and Plate Preparation, Simple Stain Procedure, 33 35 37 37 Results from the Simple Stain Procedure (Plates 7-16), Results from Prepared Slide, Laboratory Cleanup, 38 38 38 43 Bacterial Growth Lactors Needed for Bacterial Growth, Measuring Bacterial Growth, 44 45 Procedure for Preparation of Spread Plate, 45 Procedure for Demonstration of a Bacterial Growth Curve, 47 53 Gram Stain and Acid-Fast Stain The Gram Stain, 53 The Gram Stain Technique (Traditional Method) (Plate 17A, B, C), Gram Stain: (Alternative Method), 55 The Acid-Last Stain Technique (Plate 18), Acid-Last Technique, Laboratory Cleanup, Incubation, 58 58 56 56 54
Contents Discards, 58 General Cleanup, 58 Slides and Microscopes, 58 Endospore Stain, Capsule Stain, and the Hanging Drop Technique The Endospore Stain, 62 63 The Capsule Stain, 63 The Capsule Stain Technique (Plate 21), The Hanging Drop Technique, Results, 61 61 The Spore Stain Technique (Plates 19, 20), Results, VÍÍ 63 64 64 69 The Fungi, 69 Results, 72 77 Viruses—Visualization and Enumeration Viruses, 77 Bacteriophage Enumeration, Results, 78 80 85 Parasitology PART 3 Microbial Control and Biochemistry 10 97 Microbial Sensitivity Testing Part 1 : Physical Methods Ultraviolet Light Sensitivity, Sterilization by the Use of UV Light, 98 98 The Penetrating Power of UV Light (Plate 34), 99 Heat Sensitivity (may be done as an alternative to the UV light procedure), Effect of Cold Temperature and Slow Freezing (Optional), Part 2: Chemical Methods Chemical Sensitivity, 102 Chemotherapeutic Agent Testing: The Kirby-Bauer Plate, The Kirby-Bauer Technique, Results, 107 104 102 103 101
viii CONTENTS 11 113 Bacterial Biochemistry Carbohydrate Metabolizing Enzymes, Results, 114 115 Carbohydrate Fermentation (Phenol Red Broth) Tubes (Plate 37), Amino Acid and Nitrogen Metabolism, Results, 115 116 117 Decarboxylase Tubes (Plate 42), 117 123 Gas Requirements of Microorganisms Growth of Anaerobes (may be done as a demonstration), Demonstration of Catalase, 124 125 Specialized Media Examples, 129 130 Inoculation of Blood Agar Plates for Demonstration of Alpha Hemolysis and Transport Medium, 131 Inoculation of Blood, Phenylethyl Alcohol, Mannitol Salt, Macconkey, and Eosin-Methylene Blue Agar Plates, 132 Results, 133 Inoculation of Triple Sugar bon Agar and Sulfide-Indole Motility Medium, Results, 134 Results of Transport Medium, PART 4 133 134 Medical Microbiology Genetics Ames Test, 143 144 UV Light Procedure, 145 Kirby-Bauer Procedure, 145 Results: Ames Test (Plate 55), 145 Results: UV Light Procedure (Plate 35), Results: Kirby-Bauer Test (Plates 36, 55), 146 146 149 Epidemiology Handwashing Procedure (Done with Three Students), Results: Handwashing (Plate 56), 151 150
Contents Fomite and Direct Transmission of Microbes, 151 Results: Fomite and Direct Transmission, 16 152 Results: Airborne and Cough/Sneeze Plates, 153 153 Results: Microbes in Makeup, 153 Specimen-Handling Protocols Quantitative Urinalysis, 159 160 Results: Urine Samples, 162 Specimens from the Gastrointestinal Tract, Results: GI Tract Isolation Technique, 17 152 Airborne Infections: Cough and Sneeze Plates, Microbes in Makeup (Optional), 163 163 Specific Laboratory Tests Catalase Test, 169 171 Alternative Method (to be done at the end of this exercise), Results: Catalase Test (Plate 46), 171 Results: Bacitracin Sensitivity (Plate 57), Coagulase Test, 172 172 Rapid Slide Test, 172 172 Results: Coagulase Tube Test (Plate 60), Novobiocin Sensitivity, Camp Test (Plate 58), Results: Camp, 172 172 Results: Novobiocin Sensitivity, 18 172 172 Results: Oxidase Test (Plate 59), Tube Test, 171 171 Bacitracin Sensitivity—Demonstration, Oxidase Test, 173 173 173 179 Serology Elisa Test: Test for Toxins A and В from Clostridium Difficile, Results, 182 Rapid Identification of Group A Antigen, Results, 182 184 Differentiation of Streptococci Using Latex Agglutination, Slidex Strepto-Kit®, Results, ¡X 184 186 Procedure Controls, 186 184 180
x CONTENTS Identification of a Bacterial Unknown Si 191 I Identification of Enteric Pathogens: Traditional Methods Results, 194 IMViC Test (See Plate 61), 194 Results of Gelatin Hydrolysis, 194 Results of the Oxidase Test (See Plate 59), 194 identification of Enteric Pathogens: Rapid Identification Methods EnteroPluri Test (Plate 62), 201 Compartment 1: Glucose Fermentation and Gas Production, 201 Compartments 2 and 3: Lysine and Ornithine Decarboxylation, Compartment 4: Sulfide and Indole Production, 202 202 Compartments 5-8: Adonitol, Lactose, Arabinose, and Sorbitol Fermentation, Compartment 9: Voges-Proskauer, 202 Compartment 10: Phenylalanine Deaminase and Dulcitol, Compartment 11 : Urea, 202 202 202 Compartment 12: Citrate, 202 Api® 20 E System (Plate 63), 202 Microtube 1 : Ortho-Nitrophenyl-Beta-D-Ortho-Nitrophenyl-Beta-D֊Galactopyranoside (Onpg), 203 Microtubes 2-4: Arginine Dihydrolase (Decarboxylase), Lysine Decarboxylase, And Ornithine Decarboxylase (Adh, Ldc, Ode), 203 Microtube 5: Citrate (Cit), 203 Microtube 6: Sulfide (H2s), Microtube 7: Urea (Ure), 203 203 Microtube 8: Tryptophan Deaminase (Tda), Microtube 9: Indole (Ind), 203 203 Microtube 10: Voges-Proskauer (Vp), Microtube 11 : Gelatin Hydrolysis (Gel), 203 203 Microtubes 12-20: Utilization of the Carbohydrates Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Saccharose (Sucrose), Melibiose, Amygdalin, and Arabinose (Glu, Man, Ino, Sor, Rha, Sac, Mel, Amy, And Ara), 203 Inoculation of Rapid Identification Media, Inoculation of EnteroPluri Test, 204 Inoculation of the Api 20 E System, Results, 205 204 204
201
Contents xi Identification of a Bacterial Unknown: The Gram-Negative Unknown Review, 209 209 Comparison Chart, Flowchart, 210 210 Computer Identification, 210 ID Value Worksheet for EnteroPluri Test II Results, 212 213 Identification of a Bacterial Unknown: The Gram-Positive Cocci 217 PART 6 Food and Environmental Microbiology identification and Quantitation of Microbial Numbers in a Water Sample 225 Presumptive Test: Analysis of a Contaminated Water Sample for the Presence of Coliforms, 226 Results, 227 Quantitation of Microbial Number in a Water Sample, Results (Plate 64), 24 227 228 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial Numbers Microbial Presence in a Food Product and Colony Counts, Results, 232 233 Recognition of Organism Genera, 233 237 Soil Microbiology Part 1 : The Recovery of Microorganisms from Soil, Results, 238 239 Isolation Of Soil Bacteria, Results, 239 239 Part 2: Isolation of Soil Bacteriophages of Bacillus Subtilis, Results, 231 240 239
xii CONTENTS ДДДЗ Microbial Ecology 245 Competition Between Bacteria, Results, 246 Bacteriocin Production, Results, 246 246 247 Bacterial-Fungal Interaction (Plate 65), Results, 247 247 251 Biofilms Growth of Dental Plaque in situ, Results, Soil Biofilm, Results, 252 253 253 253 Growth of Bacterial Biofilms in vitro, Results, 253 254 Bibliography 261 Answer Key for the Exercise Pre-Tests 263 Appendix 1 Flow Charts and Comparison Charts for the Identification of Enteric Gram Negative Rods Using Traditional Testing Procedures 265 Appendix 2 Flow Chart for the Identification of Enterobacteriaceae based on the API 20 E Rapid Identification System 269 Appendix 3 Gram-Positive Flow Chart 275 Photo Credit List 277 Index 279 Photographic Atlas PA-1 |
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author | Pollack, Robert A. Findlay, Lorraine Mondschein, Walter Modesto, R. Ronald |
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building | Verbundindex |
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callnumber-first | Q - Science |
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callnumber-raw | QR63 |
callnumber-search | QR63 |
callnumber-sort | QR 263 |
callnumber-subject | QR - Microbiology |
classification_rvk | WC 5700 |
ctrlnum | (OCoLC)1265323900 (DE-599)BVBBV047439715 |
dewey-full | 579.078 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 579 - Microorganisms, fungi & algae |
dewey-raw | 579.078 |
dewey-search | 579.078 |
dewey-sort | 3579.078 |
dewey-tens | 570 - Biology |
discipline | Biologie |
discipline_str_mv | Biologie |
edition | Fifth edition |
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illustrated | Illustrated |
index_date | 2024-07-03T18:00:44Z |
indexdate | 2024-07-10T09:12:12Z |
institution | BVB |
isbn | 9781119462668 9781119462644 |
language | English |
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physical | xv, 284, PA-16 Seiten Illustrationen |
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publisher | Wiley |
record_format | marc |
spelling | Pollack, Robert A. Verfasser aut Laboratory exercises in microbiology Robert A. Pollack, Lorraine Findlay (PHD), Walter Mondschein (Professor Emeritis), R. Ronald Modesto (PHD) Microbiology Fifth edition Hoboken, NJ Wiley [2018] xv, 284, PA-16 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Microbiology Laboratory exercises Methode (DE-588)4038971-6 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 s Methode (DE-588)4038971-6 s DE-604 Findlay, Lorraine Verfasser aut Mondschein, Walter Verfasser aut Modesto, R. Ronald Verfasser aut Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032841910&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Pollack, Robert A. Findlay, Lorraine Mondschein, Walter Modesto, R. Ronald Laboratory exercises in microbiology Microbiology Laboratory exercises Methode (DE-588)4038971-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
subject_GND | (DE-588)4038971-6 (DE-588)4316357-9 |
title | Laboratory exercises in microbiology |
title_alt | Microbiology |
title_auth | Laboratory exercises in microbiology |
title_exact_search | Laboratory exercises in microbiology |
title_exact_search_txtP | Laboratory exercises in microbiology |
title_full | Laboratory exercises in microbiology Robert A. Pollack, Lorraine Findlay (PHD), Walter Mondschein (Professor Emeritis), R. Ronald Modesto (PHD) |
title_fullStr | Laboratory exercises in microbiology Robert A. Pollack, Lorraine Findlay (PHD), Walter Mondschein (Professor Emeritis), R. Ronald Modesto (PHD) |
title_full_unstemmed | Laboratory exercises in microbiology Robert A. Pollack, Lorraine Findlay (PHD), Walter Mondschein (Professor Emeritis), R. Ronald Modesto (PHD) |
title_short | Laboratory exercises in microbiology |
title_sort | laboratory exercises in microbiology |
topic | Microbiology Laboratory exercises Methode (DE-588)4038971-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
topic_facet | Microbiology Laboratory exercises Methode Mikrobiologie |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032841910&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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