Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures:
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| Language: | English |
| Published: |
Leipzig, Germany
Helmholtz Centre for Environmental Research - UFZ
2020
|
| Series: | Dissertation / Helmholtz-Zentrum für Umweltforschung, UFZ
2020,4 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | xxiv, 156 Seiten Illustrationen, Diagramme 25 cm |
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| 245 | 1 | 0 | |a Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |c vorgelegt von David Leuthold |
| 264 | 1 | |a Leipzig, Germany |b Helmholtz Centre for Environmental Research - UFZ |c 2020 | |
| 300 | |a xxiv, 156 Seiten |b Illustrationen, Diagramme |c 25 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Dissertation / Helmholtz-Zentrum für Umweltforschung, UFZ |v 2020,4 | |
| 502 | |b Dissertation |c RWTH Aachen |d 2020 | ||
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| adam_text | CONTENTS
1
BACKGROUND
AND
MOTIVATION
..............................................................................................
1
1.1
CHEMICALS
IN
THE
AQUATIC
ENVIRONMENT
..........................................................................
1
1.2
FISH
EMBRYO
ACUTE
TOXICITY
(FET)
TEST:
ADVANTAGES,
APPLICATIONS,
AND
LIMITATIONS
.......
2
1.3
NEUROACTIVE
CHEMICALS:
OUTSIDE
THE
APPLICABILITY
DOMAIN
OF
THE
FET
TEST
.....................
3
1.4
BEHAVIORS
IN
EMBRYONIC
AND
EARLY
LARVAL
STAGE
OF
ZEBRAFISH
AS
POTENTIAL
ENDPOINT
IN
ECOTOXICOLOGY
.................................................................................................................
3
1.5
AIM
OF
THE
THESIS:
BEHAVIOR-BASED
IDENTIFICATION
OF
ENVIRONMENTALLY
RELEVANT
CHEMICALS
AND
RELATED
MODES
OF
ACTION
.........................................................................................
5
1.5.1
ESTABLISHMENT
OF
A
LIGHT-DARK
TRANSITION
ASSAY
IN
LARVAL
ZEBRAFISH:
WHICH
PARAMETERS
NEED
TO
BE
CONSIDERED?
....................................................................
7
1.5.2
BEHAVIORAL
PROFILING
OF
ENVIRONMENTAL
CHEMICALS:
ARE
LOCOMOTOR
RESPONSES
TO
CHEMICAL
EXPOSURE
INDICATIVE
OF
MODES
OF
ACTION?
...........................................
8
1.5.3
EVALUATION
OF
UNDERLYING
MECHANISMS:
WHAT
IS
THE
BIOLOGICAL
BACKGROUND
OF
CHEMICAL-INDUCED
HYPERACTIVITY?
.......................................................................
10
1.5.4
COMBINED
EFFECTS
OF
CHEMICALS
ON
LOCOMOTOR
ACTIVITY:
IS
A
COMBINED
EFFECT
ON
LOCOMOTION
PREDICTABLE
FROM
SINGLE
COMPOUNDS
EFFECTS
AND
CAN
INDIVIDUAL
BEHAVIORAL
PROFILES
BE
RECOVERED
FROM
PROFILES
OF
MIXTURE
EXPOSURE?
..............
10
2
ESTABLISHMENT
OF
A
LIGHT-DARK
TRANSITION
ASSAY
IN
LARVAL
ZEBRAFISH
FOR
THE
ASSESSMENT
OF
ENVIRONMENTAL
CHEMICALS
...........................................................................................
17
2.1
INTRODUCTION
.................................................................................................................
18
2.2
MATERIALS
AND
METHODS
...............................................................................................
21
2.2.1
FISH
CULTURE
AND
EMBRYO
COLLECTION
....................................................................
21
2.2.2
CHEMICALS
AND
STOCK
PREPARATION
......................................................................
21
2.2.3
DETERMINATION
OFFISH
EMBRYO
ACUTE
TOXICITY
AND
CONCENTRATION-RESPONSE
MODELLING
............................................................................................................
21
2.2.4
LOCOMOTOR
RESPONSE
ASSAY
...............................................................................
22
2.2.5
STATISTICAL
ANALYSES
AND
HIERARCHICAL
CLUSTERING
.................................................
22
2.3
RESULTS
........................................................................................................................
23
2.3.1
THE
DIURNAL
RHYTHM
OF
NEWLY
HATCHED
ZEBRAFISH
LARVAE
....................................
23
2.3.1
EXPERIMENTAL
PARAMETERS
ALTER
LIGHT-DARK
TRANSITION
BEHAVIOR
..........................
23
2.3.3
CHEMICAL-INDUCED
BEHAVIORAL
ALTERATIONS
DEPEND
ON
VARIOUS
EXPOSURE
SETTINGS
...............................................................................................................
23
2.3.4
DEVELOPMENTAL
EXPOSURE
AFFECTS
ZEBRAFISH
MORPHOLOGY
....................................
28
2.4
DISCUSSION
...................................................................................................................
29
CONTENTS
XIV
3
CAN
ENVIRONMENTALLY
RELEVANT
NEUROACTIVE
CHEMICALS
BE
DETECTED
SPECIFICALLY
WITH
THE
LOCOMOTOR
RESPONSE
ASSAY
IN
ZEBRAFISH
LARVAE?
.....................................................
39
3.1
INTRODUCTION
...................................................................................................................
39
3.2
MATERIALS
AND
METHODS
................................................................................................
41
3.2.1
FISH
CULTIVATION,
EMBRYO
COLLECTION,
CHEMICALS
AND
STOCK
PREPARATION
..............
41
3.2.2
EXPERIMENTAL
DESIGN
AND
DETERMINATION
OF
EXPOSURE
CONCENTRATIONS
..............
41
3.2.3 EXPOSURE
AND
LIGHT-DARK
TRANSITION
LOCOMOTOR
RESPONSE
ASSAY
..........................
41
3.2.4
ANALYSIS
OF
LOCOMOTOR
ACTIVITY
DATA
.....................................................................
42
3.2.5
MULTIDIMENSIONAL
SCALING
(MDS)
AND
CLUSTER
ANALYSIS
........................................
42
3.3
RESULTS
.........................................................................................................................
42
3.3.1
CONCENTRATION
AND
TIME
DEPENDENCY
OF
BEHAVIORAL
PROFILES
...............................
44
3.3.2
BEHAVIORAL
PHENOTYPE-BASED
CLUSTERING
OF
CHEMICALS
.......................................
45
3.3.3
SENSITIVITY
OF
LOCOMOTOR
RESPONSES
IN
RELATION
TO
LETHAL
ACUTE
TOXICITY
..............
49
3.4
DISCUSSION
....................................................................................................................
54
4
CO-OCCURRENCE
OF
ACETYLCHOLINESTERASE
INHIBITION
AND
BRADYCARDIA
IS
ASSOCIATED
BUT
NOT
CAUSALLY
LINKED
WITH
HYPERLOCOMOTION
IN
ZEBRAFISH
LARVAE
AFTER
CHEMICAL
EXPOSURE
............................................................................................................................
65
4.1
INTRODUCTION
...................................................................................................................
65
4.2
MATERIALS
AND
METHODS
................................................................................................
67
4.2.1
FISH
CULTIVATION
AND
EMBRYO
COLLECTION
...............................................................
67
4.2.2
CHEMICALS
AND
STOCK
PREPARATION
.......................................................................
68
4.2.3
EXPOSURE
AND
PRE-INCUBATION
..............................................................................
68
4.2.4
LIGHT-DARK
TRANSITION
ASSAY
AND
ANALYSIS
OF
LOCOMOTOR
ACTIVITY
DATA
...................
68
4.2.5
QUANTIFICATION
OF
ACETYLCHOLINESTERASE
ACTIVITY
....................................................
69
4.2.6
HEART
RATE
DETERMINATION
.....................................................................................
69
4.2.7
HIERARCHICAL
CLUSTERING
AND
MULTIDIMENSIONAL
SCALING
........................................
70
4.3
RESULTS
.........................................................................................................................
70
4.3.1
ACETYLCHOLINESTERASE
INHIBITORS
AND
HERBICIDES
CAUSE
A
HYPERACTIVE
LOCOMOTOR
PHENOTYPE
...........................................................................................................
70
4.3.2
INHIBITION
OF
ACETYLCHOLINESTERASE
IS
NOT
CORRELATED
WITH
HYPERLOCOMOTION
........
72
4.3.3
ACETYLCHOLINESTERASE
INHIBITORS
AND
HERBICIDES
CAUSE
BRADYCARDIA
...................
75
4.3.4
CARDIOSELECTIVE
DRUGS
ONLY
PARTIALLY
COPY
HYPERACTIVE
PHENOTYPES
...................
76
4.3.5
HEART
RATE
AND
LOCOMOTOR
ACTIVITY
ARE
PARTIALLY
CO-REGULATED
..............................
78
4.4
DISCUSSION
....................................................................................................................
78
CONTENTS
5
COMBINED
EFFECTS
OF
CHEMICAL
MIXTURES
ON
LOCOMOTOR
ACTIVITY:
PREDICTION
AND
DIAGNOSTIC
POTENTIAL
OF
BEHAVIORAL
ALTERATIONS
IN
LARVAL
ZEBRAFISH
...............................
91
5.1
INTRODUCTION
.................................................................................................................
91
5.2
MATERIALS
AND
METHODS
...............................................................................................
93
5.2.1
FISH
CULTIVATION
AND
EMBRYO
COLLECTION
...............................................................
93
5.2.2
CHEMICALS
AND
STOCK
PREPARATION
......................................................................
93
5.2.3
MIXTURE
DESIGN
AND
MIXTURE
EFFECT
PREDICTION
.....................................................
94
5.2.4
EXPOSURE,
LOCOMOTOR
RESPONSE
ASSAY,
AND
DATA
ANALYSIS
................................
95
5.2.5
MULTIDIMENSIONAL
SCALING
....................................................................................
95
5.2.6
QUANTIFICATION
OF
ACETYLCHOLINESTERASE
ACTIVITY,
HEART
RATE,
AND
MORPHOLOGICAL
FEATURES
..............................................................................................................
95
5.3
RESULTS
........................................................................................................................
96
5.3.1
CLASSICAL
CONCENTRATION
RESPONSE
MODELS
ARE
APPLICABLE
TO
LOCOMOTOR
ACTIVITY
...............................................................................................................
96
5.3.2
MIXTURES
OF
CHEMICALS
ACT
ADDITIVELY
ON
LOCOMOTOR
ACTIVITY
..............................
97
5.3.3
DESIGN
OF
AN
ENVIRONMENTALLY
RELEVANT
MIXTURE
TO
EXAMINE
THE
DIAGNOSTIC
POTENTIAL
OF
LOCOMOTOR
RESPONSE
PATTERNS
.........................................................
100
5.3.4
DETERMINATION
OFCOX
INHIBITOR-SPECIFIC
BEHAVIORAL
PHENOTYPES
.....................
101
5.3.5
BEHAVIORAL
PHENOTYPES
DETERMINED
WITH
A
QUINARY
MIXTURE
OF
CHEMICALS
RESEMBLE
DISTINCT
PROFILES
OF
INDIVIDUAL
COMPONENTS
........................................
103
5.4
DISCUSSION
.................................................................................................................
105
6
CONCLUSIONS
AND
FUTURE
PERSPECTIVES
..........................................................................
115
6.1
BEHAVIORAL
ENDPOINTS
IN
THE
EFFECT-BASED
ASSESSMENT
OF
CHEMICALS
AND
MIXTURES
..116
6.2
DIAGNOSTIC
CAPACITY
OF
BEHAVIORAL
PHENOTYPES
..........................................................
119
APPENDIX
.............................................................................................................................
123
A
SUPPORTING
INFORMATION
-
CHAPTER
2
...........................................................................
124
B
SUPPORTING
INFORMATION
-
CHAPTER
3
...........................................................................
131
C
SUPPORTING
INFORMATION
-
CHAPTER
4
...........................................................................
143
D
SUPPORTING
INFORMATION
-
CHAPTER
5
...........................................................................
151
XV
|
| adam_txt |
CONTENTS
1
BACKGROUND
AND
MOTIVATION
.
1
1.1
CHEMICALS
IN
THE
AQUATIC
ENVIRONMENT
.
1
1.2
FISH
EMBRYO
ACUTE
TOXICITY
(FET)
TEST:
ADVANTAGES,
APPLICATIONS,
AND
LIMITATIONS
.
2
1.3
NEUROACTIVE
CHEMICALS:
OUTSIDE
THE
APPLICABILITY
DOMAIN
OF
THE
FET
TEST
.
3
1.4
BEHAVIORS
IN
EMBRYONIC
AND
EARLY
LARVAL
STAGE
OF
ZEBRAFISH
AS
POTENTIAL
ENDPOINT
IN
ECOTOXICOLOGY
.
3
1.5
AIM
OF
THE
THESIS:
BEHAVIOR-BASED
IDENTIFICATION
OF
ENVIRONMENTALLY
RELEVANT
CHEMICALS
AND
RELATED
MODES
OF
ACTION
.
5
1.5.1
ESTABLISHMENT
OF
A
LIGHT-DARK
TRANSITION
ASSAY
IN
LARVAL
ZEBRAFISH:
WHICH
PARAMETERS
NEED
TO
BE
CONSIDERED?
.
7
1.5.2
BEHAVIORAL
PROFILING
OF
ENVIRONMENTAL
CHEMICALS:
ARE
LOCOMOTOR
RESPONSES
TO
CHEMICAL
EXPOSURE
INDICATIVE
OF
MODES
OF
ACTION?
.
8
1.5.3
EVALUATION
OF
UNDERLYING
MECHANISMS:
WHAT
IS
THE
BIOLOGICAL
BACKGROUND
OF
CHEMICAL-INDUCED
HYPERACTIVITY?
.
10
1.5.4
COMBINED
EFFECTS
OF
CHEMICALS
ON
LOCOMOTOR
ACTIVITY:
IS
A
COMBINED
EFFECT
ON
LOCOMOTION
PREDICTABLE
FROM
SINGLE
COMPOUNDS'
EFFECTS
AND
CAN
INDIVIDUAL
BEHAVIORAL
PROFILES
BE
RECOVERED
FROM
PROFILES
OF
MIXTURE
EXPOSURE?
.
10
2
ESTABLISHMENT
OF
A
LIGHT-DARK
TRANSITION
ASSAY
IN
LARVAL
ZEBRAFISH
FOR
THE
ASSESSMENT
OF
ENVIRONMENTAL
CHEMICALS
.
17
2.1
INTRODUCTION
.
18
2.2
MATERIALS
AND
METHODS
.
21
2.2.1
FISH
CULTURE
AND
EMBRYO
COLLECTION
.
21
2.2.2
CHEMICALS
AND
STOCK
PREPARATION
.
21
2.2.3
DETERMINATION
OFFISH
EMBRYO
ACUTE
TOXICITY
AND
CONCENTRATION-RESPONSE
MODELLING
.
21
2.2.4
LOCOMOTOR
RESPONSE
ASSAY
.
22
2.2.5
STATISTICAL
ANALYSES
AND
HIERARCHICAL
CLUSTERING
.
22
2.3
RESULTS
.
23
2.3.1
THE
DIURNAL
RHYTHM
OF
NEWLY
HATCHED
ZEBRAFISH
LARVAE
.
23
2.3.1
EXPERIMENTAL
PARAMETERS
ALTER
LIGHT-DARK
TRANSITION
BEHAVIOR
.
23
2.3.3
CHEMICAL-INDUCED
BEHAVIORAL
ALTERATIONS
DEPEND
ON
VARIOUS
EXPOSURE
SETTINGS
.
23
2.3.4
DEVELOPMENTAL
EXPOSURE
AFFECTS
ZEBRAFISH
MORPHOLOGY
.
28
2.4
DISCUSSION
.
29
CONTENTS
XIV
3
CAN
ENVIRONMENTALLY
RELEVANT
NEUROACTIVE
CHEMICALS
BE
DETECTED
SPECIFICALLY
WITH
THE
LOCOMOTOR
RESPONSE
ASSAY
IN
ZEBRAFISH
LARVAE?
.
39
3.1
INTRODUCTION
.
39
3.2
MATERIALS
AND
METHODS
.
41
3.2.1
FISH
CULTIVATION,
EMBRYO
COLLECTION,
CHEMICALS
AND
STOCK
PREPARATION
.
41
3.2.2
EXPERIMENTAL
DESIGN
AND
DETERMINATION
OF
EXPOSURE
CONCENTRATIONS
.
41
3.2.3 EXPOSURE
AND
LIGHT-DARK
TRANSITION
LOCOMOTOR
RESPONSE
ASSAY
.
41
3.2.4
ANALYSIS
OF
LOCOMOTOR
ACTIVITY
DATA
.
42
3.2.5
MULTIDIMENSIONAL
SCALING
(MDS)
AND
CLUSTER
ANALYSIS
.
42
3.3
RESULTS
.
42
3.3.1
CONCENTRATION
AND
TIME
DEPENDENCY
OF
BEHAVIORAL
PROFILES
.
44
3.3.2
BEHAVIORAL
PHENOTYPE-BASED
CLUSTERING
OF
CHEMICALS
.
45
3.3.3
SENSITIVITY
OF
LOCOMOTOR
RESPONSES
IN
RELATION
TO
LETHAL
ACUTE
TOXICITY
.
49
3.4
DISCUSSION
.
54
4
CO-OCCURRENCE
OF
ACETYLCHOLINESTERASE
INHIBITION
AND
BRADYCARDIA
IS
ASSOCIATED
BUT
NOT
CAUSALLY
LINKED
WITH
HYPERLOCOMOTION
IN
ZEBRAFISH
LARVAE
AFTER
CHEMICAL
EXPOSURE
.
65
4.1
INTRODUCTION
.
65
4.2
MATERIALS
AND
METHODS
.
67
4.2.1
FISH
CULTIVATION
AND
EMBRYO
COLLECTION
.
67
4.2.2
CHEMICALS
AND
STOCK
PREPARATION
.
68
4.2.3
EXPOSURE
AND
PRE-INCUBATION
.
68
4.2.4
LIGHT-DARK
TRANSITION
ASSAY
AND
ANALYSIS
OF
LOCOMOTOR
ACTIVITY
DATA
.
68
4.2.5
QUANTIFICATION
OF
ACETYLCHOLINESTERASE
ACTIVITY
.
69
4.2.6
HEART
RATE
DETERMINATION
.
69
4.2.7
HIERARCHICAL
CLUSTERING
AND
MULTIDIMENSIONAL
SCALING
.
70
4.3
RESULTS
.
70
4.3.1
ACETYLCHOLINESTERASE
INHIBITORS
AND
HERBICIDES
CAUSE
A
HYPERACTIVE
LOCOMOTOR
PHENOTYPE
.
70
4.3.2
INHIBITION
OF
ACETYLCHOLINESTERASE
IS
NOT
CORRELATED
WITH
HYPERLOCOMOTION
.
72
4.3.3
ACETYLCHOLINESTERASE
INHIBITORS
AND
HERBICIDES
CAUSE
BRADYCARDIA
.
75
4.3.4
CARDIOSELECTIVE
DRUGS
ONLY
PARTIALLY
COPY
HYPERACTIVE
PHENOTYPES
.
76
4.3.5
HEART
RATE
AND
LOCOMOTOR
ACTIVITY
ARE
PARTIALLY
CO-REGULATED
.
78
4.4
DISCUSSION
.
78
CONTENTS
5
COMBINED
EFFECTS
OF
CHEMICAL
MIXTURES
ON
LOCOMOTOR
ACTIVITY:
PREDICTION
AND
DIAGNOSTIC
POTENTIAL
OF
BEHAVIORAL
ALTERATIONS
IN
LARVAL
ZEBRAFISH
.
91
5.1
INTRODUCTION
.
91
5.2
MATERIALS
AND
METHODS
.
93
5.2.1
FISH
CULTIVATION
AND
EMBRYO
COLLECTION
.
93
5.2.2
CHEMICALS
AND
STOCK
PREPARATION
.
93
5.2.3
MIXTURE
DESIGN
AND
MIXTURE
EFFECT
PREDICTION
.
94
5.2.4
EXPOSURE,
LOCOMOTOR
RESPONSE
ASSAY,
AND
DATA
ANALYSIS
.
95
5.2.5
MULTIDIMENSIONAL
SCALING
.
95
5.2.6
QUANTIFICATION
OF
ACETYLCHOLINESTERASE
ACTIVITY,
HEART
RATE,
AND
MORPHOLOGICAL
FEATURES
.
95
5.3
RESULTS
.
96
5.3.1
CLASSICAL
CONCENTRATION
RESPONSE
MODELS
ARE
APPLICABLE
TO
LOCOMOTOR
ACTIVITY
.
96
5.3.2
MIXTURES
OF
CHEMICALS
ACT
ADDITIVELY
ON
LOCOMOTOR
ACTIVITY
.
97
5.3.3
DESIGN
OF
AN
ENVIRONMENTALLY
RELEVANT
MIXTURE
TO
EXAMINE
THE
DIAGNOSTIC
POTENTIAL
OF
LOCOMOTOR
RESPONSE
PATTERNS
.
100
5.3.4
DETERMINATION
OFCOX
INHIBITOR-SPECIFIC
BEHAVIORAL
PHENOTYPES
.
101
5.3.5
BEHAVIORAL
PHENOTYPES
DETERMINED
WITH
A
QUINARY
MIXTURE
OF
CHEMICALS
RESEMBLE
DISTINCT
PROFILES
OF
INDIVIDUAL
COMPONENTS
.
103
5.4
DISCUSSION
.
105
6
CONCLUSIONS
AND
FUTURE
PERSPECTIVES
.
115
6.1
BEHAVIORAL
ENDPOINTS
IN
THE
EFFECT-BASED
ASSESSMENT
OF
CHEMICALS
AND
MIXTURES
.116
6.2
DIAGNOSTIC
CAPACITY
OF
BEHAVIORAL
PHENOTYPES
.
119
APPENDIX
.
123
A
SUPPORTING
INFORMATION
-
CHAPTER
2
.
124
B
SUPPORTING
INFORMATION
-
CHAPTER
3
.
131
C
SUPPORTING
INFORMATION
-
CHAPTER
4
.
143
D
SUPPORTING
INFORMATION
-
CHAPTER
5
.
151
XV |
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| any_adam_object_boolean | 1 |
| author | Leuthold, David 1989- |
| author_GND | (DE-588)1221607928 |
| author_facet | Leuthold, David 1989- |
| author_role | aut |
| author_sort | Leuthold, David 1989- |
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| building | Verbundindex |
| bvnumber | BV047381907 |
| ctrlnum | (OCoLC)1226659087 (DE-599)DNB1223879879 |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047381907 |
| illustrated | Illustrated |
| index_date | 2024-07-03T17:47:51Z |
| indexdate | 2024-07-10T09:10:34Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032783450 |
| oclc_num | 1226659087 |
| open_access_boolean | |
| owner | DE-83 DE-634 DE-355 DE-BY-UBR |
| owner_facet | DE-83 DE-634 DE-355 DE-BY-UBR |
| physical | xxiv, 156 Seiten Illustrationen, Diagramme 25 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Helmholtz Centre for Environmental Research - UFZ |
| record_format | marc |
| series2 | Dissertation / Helmholtz-Zentrum für Umweltforschung, UFZ |
| spelling | Leuthold, David 1989- Verfasser (DE-588)1221607928 aut Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures vorgelegt von David Leuthold Leipzig, Germany Helmholtz Centre for Environmental Research - UFZ 2020 xxiv, 156 Seiten Illustrationen, Diagramme 25 cm txt rdacontent n rdamedia nc rdacarrier Dissertation / Helmholtz-Zentrum für Umweltforschung, UFZ 2020,4 Dissertation RWTH Aachen 2020 Zebrabärbling (DE-588)4079468-4 gnd rswk-swf Neurotoxizität (DE-588)4123836-9 gnd rswk-swf Verhalten (DE-588)4062860-7 gnd rswk-swf Umweltchemikalie (DE-588)4117281-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zebrabärbling (DE-588)4079468-4 s Verhalten (DE-588)4062860-7 s Umweltchemikalie (DE-588)4117281-4 s Neurotoxizität (DE-588)4123836-9 s DE-604 Helmholtz-Zentrum für Umweltforschung, UFZ Dissertation 2020,4 (DE-604)BV022864787 2020,4 B:DE-101 application/pdf https://d-nb.info/1223879879/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032783450&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Leuthold, David 1989- Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures Zebrabärbling (DE-588)4079468-4 gnd Neurotoxizität (DE-588)4123836-9 gnd Verhalten (DE-588)4062860-7 gnd Umweltchemikalie (DE-588)4117281-4 gnd |
| subject_GND | (DE-588)4079468-4 (DE-588)4123836-9 (DE-588)4062860-7 (DE-588)4117281-4 (DE-588)4113937-9 |
| title | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |
| title_auth | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |
| title_exact_search | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |
| title_exact_search_txtP | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |
| title_full | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures vorgelegt von David Leuthold |
| title_fullStr | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures vorgelegt von David Leuthold |
| title_full_unstemmed | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures vorgelegt von David Leuthold |
| title_short | Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures |
| title_sort | zebrafish locomotor activity as a sensitive effect based tool for the assessment of environmental chemicals and mixtures |
| topic | Zebrabärbling (DE-588)4079468-4 gnd Neurotoxizität (DE-588)4123836-9 gnd Verhalten (DE-588)4062860-7 gnd Umweltchemikalie (DE-588)4117281-4 gnd |
| topic_facet | Zebrabärbling Neurotoxizität Verhalten Umweltchemikalie Hochschulschrift |
| url | https://d-nb.info/1223879879/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032783450&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV022864787 |
| work_keys_str_mv | AT leutholddavid zebrafishlocomotoractivityasasensitiveeffectbasedtoolfortheassessmentofenvironmentalchemicalsandmixtures |
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