Specific roles of poly(a) polymerase1 in the male gametophyte and beyond:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Potsdam
[2019]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 143 Blätter Illustrationen 30 cm |
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| 245 | 1 | 0 | |a Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |c von Anna Ramming |
| 264 | 1 | |a Potsdam |c [2019] | |
| 300 | |a 143 Blätter |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Universität Potsdam |d 2019 | ||
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Datensatz im Suchindex
| _version_ | 1804182549527789568 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.................................................................................................................................
3
SUMMARY
...............................................................................................................................................
6
ZUSAMMENFASSUNG
...............................................................................................................................
8
LIST
OF
FIGURES
......................................................................................................................................
10
LIST
OF
TABLES
.......................................................................................................................................
13
ABBREVIATIONS
......................................................................................................................................
14
1
INTRODUCTION
...............................................................................................................................
18
1.1
MRNA-PROCESSING:
CAPPING,
SPLICING,
AND
POLYADENYLATION
............................................
18
1.1.1
CAPPING
.......................................................................................................................
18
1.1.2
SPLICING
.......................................................................................................................
19
1.1.3
POLYADENYLATION
.........................................................................................................
19
1.2
POLY(A)
POLYMERASES,
PAP(S)
............................................................................................
29
1.2.1
PAPS
IN
ARABIDOPSIS
THALIANA
....................................................................................
31
1.2.2
PAPS1
AND
PAPS2/4
EXHIBIT
SPECIALIZED
FUNCTIONS
.................................................
34
1.2.3
PAPS1
POTENTIALLY
CONFERS
COMPETENCY
TO
POLLEN
TUBES
........................................
36
1.3
FERTILIZATION
IN
ANGIOSPERMS
..............................................................................................
38
1.3.1
POLLEN
TUBE
GERMINATION
AND
GROWTH
.....................................................................
39
1.3.2
SIGNALS
AND
MECHANISMS
DURING
PT
GUIDANCE
AND
FERTILIZATION
.............................
42
1.4
AIMS
OF
THIS
STUDY
..............................................................................................................
59
2
MATERIAL
AND
METHODS
...............................................................................................................
60
2.1
MATERIAL
.............................................................................................................................
60
2.1.1
PLANTS
..........................................................................................................................
62
2.1.2
MICROORGANISMS
.........................................................................................................
62
2.1.3 SELECTIVE
ANTIBIOTICS
AND
HERBICIDES
..........................................................................
63
2.2 PLANT
CULTIVATION
................................................................................................................
63
TABLE
OF
CONTENTS
2.2.1
PLANT
GROWTH
CONDITIONS
..........................................................................................
63
2.2.2
SEED
STERILIZATION
.......................................................................................................
63
2.2.3
EMASCULATION
OF
PLANTS
.............................................................................................
64
2.2.4
CROSSING
OF
PLANTS
......................................................................................................
64
2.3
GENOTYPING
.........................................................................................................................
64
2.3.1
DNA
EXTRACTION
..........................................................................................................
64
2.3.2
POLYMERASE-CHAIN-REACTION
......................................................................................
65
2.3.3
GEL
ELECTROPHORESIS
OF
DNA
FRAGMENTS
....................................................................
66
2.3.4
GENOTYPING
BY
KBIOSCIENCES
COMPETITIVE
ALLELE-SPECIFIC
PCR
(KASP)
....................
66
2.3.5
DETERMINATION
OF
NUCLEIC
ACID
CONCENTRATIONS
......................................................
67
2.4
POLLEN
TUBE
ASSAYS
............................................................................................................
67
2.4.1
IN
VITRO
PERFORMED
POLLEN
TUBE
GROWTH
ASSAY
........................................................
67
2.4.2
SEMI-/N
VIVO
PERFORMED
POLLEN
TUBE
GROWTH
ASSAY
................................................
68
2.4.3
ATLURE
SENSITIVITY
ASSAY
OF
POLLEN
TUBES
................................................................
68
2.4.4
MICROSCOPY
................................................................................................................
69
2.4.5
4
,
6-DIAMIDIN-2-PHENYLINDOL
(DAPI)
STAINING
......................................................
69
2.4.6
ANILINE
BLUE
STAINING
OF
POLLEN
TUBES
......................................................................
69
2.4.7
POLLEN
TUBE
LENGTH
EVALUATION
................................................
70
2.5
RNA
ISOLATION,
LIBRARY
PREPARATION,
AND
SEQUENCING
......................................................70
2.6
SOFTWARE
AND
ONLINE
TOOLS
USED
IN
THIS
WORK
...................................................................
71
3
PUBLICATIONS
................................................................................................................................
73
4
RESULTS
........................................................................................................................................
74
4.1
PAPSL-3
POLLEN
GRAINS
EXHIBIT
SIZE
ABNORMALITIES
...........................................................
74
4.2
PAPSL-3
POLLEN
GRAINS
CONTAIN
ABNORMAL
NUMBER
OF
CELL
NUCLEI
..................................
77
4.3
PPAPSL::PAPSL-YFP
IS
UPREGULATED
BY
GROWTH
THROUGH
STYLE
........................................
78
4.4
MIXED
POLLINATION
ASSAY
WITH
PAPSL-L/+
PLANTS
REVEALED
NON-RANDOM
MATING
IN
DEPENDENCE
OF
THE
OVULE S
POSITION
............................................................................................
82
4.5
GERMINATION
AND
PT
GROWTH
RATE
OF
PAPSL-1
IS
UNAFFECTED
.......................................... 86
4
TABLE
OF
CONTENTS
4.5.1
GERMINATION
RATE
IN
VITRO
.........................................................................................
87
4.5.2
POLLEN
TUBE
GROWTH
RATE
SEMI-/N
VIVO
.....................................................................
87
4.5.3
POLLEN
TUBE
GROWTH
RATE
IN
VIVO
..............................................................................90
4.6
POLLEN
TUBE
EMERGENCE
IS
NOT
AFFECTED
IN
PAPSL-1
POLLEN
TUBES
..................................
92
4.7
IN
VIVO
GUIDANCE
IS
INDEED
AFFECTED
IN
PAPSL-1
MUTANT
POLLEN
TUBES
...........................
94
4.8
PAPSL-1
POLLEN
TUBES
DISPLAY
NORMAL
ATLURE
SENSITIVITY
AND
REACTION
......................
103
4.9
TRANSCRIPTOMIC
ANALYZES
OF
PAPS
MUTANT
MALE
GAMETOPHYTES
....................................
104
4.9.1
RNA-SEQ
OF
PAPS1
PLAT52::RFP
/
PAPSL-3
SORTED
POLLEN
GRAINS
..........................
105
4.9.2
RNA-SEQ
OF
POLLEN
TUBE
-
STYLE
-
COMPLEXES
........................................................
107
5
DISCUSSION
.................................................................................................................................
109
REFERENCES
........................................................................................................................................
120
ACKNOWLEDGEMENTS
..........................................................................................................................
141
AFFIDAVIT
.............................................................................................................................................
142
5
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.
3
SUMMARY
.
6
ZUSAMMENFASSUNG
.
8
LIST
OF
FIGURES
.
10
LIST
OF
TABLES
.
13
ABBREVIATIONS
.
14
1
INTRODUCTION
.
18
1.1
MRNA-PROCESSING:
CAPPING,
SPLICING,
AND
POLYADENYLATION
.
18
1.1.1
CAPPING
.
18
1.1.2
SPLICING
.
19
1.1.3
POLYADENYLATION
.
19
1.2
POLY(A)
POLYMERASES,
PAP(S)
.
29
1.2.1
PAPS
IN
ARABIDOPSIS
THALIANA
.
31
1.2.2
PAPS1
AND
PAPS2/4
EXHIBIT
SPECIALIZED
FUNCTIONS
.
34
1.2.3
PAPS1
POTENTIALLY
CONFERS
COMPETENCY
TO
POLLEN
TUBES
.
36
1.3
FERTILIZATION
IN
ANGIOSPERMS
.
38
1.3.1
POLLEN
TUBE
GERMINATION
AND
GROWTH
.
39
1.3.2
SIGNALS
AND
MECHANISMS
DURING
PT
GUIDANCE
AND
FERTILIZATION
.
42
1.4
AIMS
OF
THIS
STUDY
.
59
2
MATERIAL
AND
METHODS
.
60
2.1
MATERIAL
.
60
2.1.1
PLANTS
.
62
2.1.2
MICROORGANISMS
.
62
2.1.3 SELECTIVE
ANTIBIOTICS
AND
HERBICIDES
.
63
2.2 PLANT
CULTIVATION
.
63
TABLE
OF
CONTENTS
2.2.1
PLANT
GROWTH
CONDITIONS
.
63
2.2.2
SEED
STERILIZATION
.
63
2.2.3
EMASCULATION
OF
PLANTS
.
64
2.2.4
CROSSING
OF
PLANTS
.
64
2.3
GENOTYPING
.
64
2.3.1
DNA
EXTRACTION
.
64
2.3.2
POLYMERASE-CHAIN-REACTION
.
65
2.3.3
GEL
ELECTROPHORESIS
OF
DNA
FRAGMENTS
.
66
2.3.4
GENOTYPING
BY
KBIOSCIENCES
COMPETITIVE
ALLELE-SPECIFIC
PCR
(KASP)
.
66
2.3.5
DETERMINATION
OF
NUCLEIC
ACID
CONCENTRATIONS
.
67
2.4
POLLEN
TUBE
ASSAYS
.
67
2.4.1
IN
VITRO
PERFORMED
POLLEN
TUBE
GROWTH
ASSAY
.
67
2.4.2
SEMI-/N
VIVO
PERFORMED
POLLEN
TUBE
GROWTH
ASSAY
.
68
2.4.3
ATLURE
SENSITIVITY
ASSAY
OF
POLLEN
TUBES
.
68
2.4.4
MICROSCOPY
.
69
2.4.5
4'
,
6-DIAMIDIN-2-PHENYLINDOL
(DAPI)
STAINING
.
69
2.4.6
ANILINE
BLUE
STAINING
OF
POLLEN
TUBES
.
69
2.4.7
POLLEN
TUBE
LENGTH
EVALUATION
.
70
2.5
RNA
ISOLATION,
LIBRARY
PREPARATION,
AND
SEQUENCING
.70
2.6
SOFTWARE
AND
ONLINE
TOOLS
USED
IN
THIS
WORK
.
71
3
PUBLICATIONS
.
73
4
RESULTS
.
74
4.1
PAPSL-3
POLLEN
GRAINS
EXHIBIT
SIZE
ABNORMALITIES
.
74
4.2
PAPSL-3
POLLEN
GRAINS
CONTAIN
ABNORMAL
NUMBER
OF
CELL
NUCLEI
.
77
4.3
PPAPSL::PAPSL-YFP
IS
UPREGULATED
BY
GROWTH
THROUGH
STYLE
.
78
4.4
MIXED
POLLINATION
ASSAY
WITH
PAPSL-L/+
PLANTS
REVEALED
NON-RANDOM
MATING
IN
DEPENDENCE
OF
THE
OVULE'S
POSITION
.
82
4.5
GERMINATION
AND
PT
GROWTH
RATE
OF
PAPSL-1
IS
UNAFFECTED
. 86
4
TABLE
OF
CONTENTS
4.5.1
GERMINATION
RATE
IN
VITRO
.
87
4.5.2
POLLEN
TUBE
GROWTH
RATE
SEMI-/N
VIVO
.
87
4.5.3
POLLEN
TUBE
GROWTH
RATE
IN
VIVO
.90
4.6
POLLEN
TUBE
EMERGENCE
IS
NOT
AFFECTED
IN
PAPSL-1
POLLEN
TUBES
.
92
4.7
IN
VIVO
GUIDANCE
IS
INDEED
AFFECTED
IN
PAPSL-1
MUTANT
POLLEN
TUBES
.
94
4.8
PAPSL-1
POLLEN
TUBES
DISPLAY
NORMAL
ATLURE
SENSITIVITY
AND
REACTION
.
103
4.9
TRANSCRIPTOMIC
ANALYZES
OF
PAPS
MUTANT
MALE
GAMETOPHYTES
.
104
4.9.1
RNA-SEQ
OF
PAPS1
PLAT52::RFP
/
PAPSL-3
SORTED
POLLEN
GRAINS
.
105
4.9.2
RNA-SEQ
OF
POLLEN
TUBE
-
STYLE
-
COMPLEXES
.
107
5
DISCUSSION
.
109
REFERENCES
.
120
ACKNOWLEDGEMENTS
.
141
AFFIDAVIT
.
142
5 |
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| author_GND | (DE-588)1202480705 |
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| illustrated | Illustrated |
| index_date | 2024-07-03T17:32:46Z |
| indexdate | 2024-07-10T09:09:19Z |
| institution | BVB |
| language | English |
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| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 143 Blätter Illustrationen 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Ramming, Anna 1988- Verfasser (DE-588)1202480705 aut Specific roles of poly(a) polymerase1 in the male gametophyte and beyond von Anna Ramming Potsdam [2019] 143 Blätter Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Potsdam 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1203565429/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032738756&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ramming, Anna 1988- Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| subject_GND | (DE-588)4113937-9 |
| title | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| title_auth | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| title_exact_search | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| title_exact_search_txtP | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| title_full | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond von Anna Ramming |
| title_fullStr | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond von Anna Ramming |
| title_full_unstemmed | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond von Anna Ramming |
| title_short | Specific roles of poly(a) polymerase1 in the male gametophyte and beyond |
| title_sort | specific roles of poly a polymerase1 in the male gametophyte and beyond |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1203565429/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032738756&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ramminganna specificrolesofpolyapolymerase1inthemalegametophyteandbeyond |
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