Plant Endosomes Methods and Protocols (Springer US, 2020)
Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis -- Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles -- Analysis of Endoplasmic Reticulum-Endosomes Association Using Live-Cell Imaging In Plant Cells -- Degradation of Abscisic Acid Receptors through the Endosomal Pathway -- Biochemical and Imaging Analysis of ALIX Function in the Endosomal Trafficking of Arabidopsis Protein Cargoes -- Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network -- Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy -- Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking -- Assessing Extrinsic Membrane Protein Dependency to PI4P using a Plasma Membrane to Endosome Re-localisation Transient Assay in Nicotiana benthamiana -- Subcellular Localization of PI3P in Arabidopsis -- Immuno-purification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis -- Cell-free Protein Translation System For Expression Of Lipid-binding Proteins Tagged with Small Epitopes and their use in Protein-lipid Overlay Assays -- Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants -- Detection of Phosphorylation on Immunoprecipitates from Total Protein Extracts of Arabidopsis thaliana Seedlings -- Purification and Interaction Analysis of a Plant-specific RAB5 Effector by in vitro pull-down Assay.