Plant Cold Acclimation Methods and Protocols (Springer US, 2020)
Introduction: Plant Cold Acclimation and Winter Survival -- Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays -- Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements -- Infrared Thermal Analysis of Plant Freezing Processes -- Conducting Field Trials for Frost Tolerance Breeding in Cereals -- A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes -- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation -- Identification of Arabidopsis Mutants with Altered Freezing Tolerance -- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues -- Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue -- Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography -- Proteomic Approaches to Identify Proteins Responsive to Cold Stress -- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins -- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition -- Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry -- Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation -- Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation -- Subcellular Compartmentation of Metabolites Involved in Cold Acclimation -- Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime -- Characterization of Ice Binding Proteins from Sea-Ice Microalgae -- Isolation and Characterization of Ice-Binding Proteins from Higher Plants