Plant Meiosis: Methods and Protocols
This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polypl...
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| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2020
|
| Ausgabe: | 1st ed. 2020 |
| Schriftenreihe: | Methods in Molecular Biology
2061 |
| Schlagworte: | |
| Online-Zugang: | TUM01 UBR01 Volltext |
| Zusammenfassung: | This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. |
| Beschreibung: | A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- - Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for "-omics" Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- - A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes - EdU as Compound Example |
| ISBN: | 9781493998180 |
| DOI: | 10.1007/978-1-4939-9818-0 |
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| 520 | |a This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. | ||
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Datensatz im Suchindex
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| dewey-ones | 580 - Plants |
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| edition | 1st ed. 2020 |
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| spelling | Plant Meiosis Methods and Protocols edited by Mónica Pradillo, Stefan Heckmann 1st ed. 2020 New York, NY Springer New York 2020 txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2061 A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana -- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM -- Targeted Analysis of Chromatin Events (TACE) -- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) -- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis -- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn -- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants -- - Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids -- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy -- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium -- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy -- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers -- Isolating Male Meiocytes from Maize and Wheat for "-omics" Analyses -- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen -- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development -- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei -- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops -- - A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) -- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana -- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species -- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants -- A Modular Tray Growth System for Barley -- In planta Delivery of Chemical Compounds into Barley Meiocytes - EdU as Compound Example This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. Plant science Botany Pradillo, Mónica Sonstige oth Heckmann, Stefan Sonstige oth Erscheint auch als Druck-Ausgabe 9781493998173 Erscheint auch als Druck-Ausgabe 9781493998197 Erscheint auch als Druck-Ausgabe 9781493998203 https://doi.org/10.1007/978-1-4939-9818-0 Verlag URL des Erstveröffentlichers Volltext text file PDF |
| spellingShingle | Plant Meiosis Methods and Protocols Plant science Botany |
| title | Plant Meiosis Methods and Protocols |
| title_auth | Plant Meiosis Methods and Protocols |
| title_exact_search | Plant Meiosis Methods and Protocols |
| title_exact_search_txtP | Plant Meiosis Methods and Protocols |
| title_full | Plant Meiosis Methods and Protocols edited by Mónica Pradillo, Stefan Heckmann |
| title_fullStr | Plant Meiosis Methods and Protocols edited by Mónica Pradillo, Stefan Heckmann |
| title_full_unstemmed | Plant Meiosis Methods and Protocols edited by Mónica Pradillo, Stefan Heckmann |
| title_short | Plant Meiosis |
| title_sort | plant meiosis methods and protocols |
| title_sub | Methods and Protocols |
| topic | Plant science Botany |
| topic_facet | Plant science Botany |
| url | https://doi.org/10.1007/978-1-4939-9818-0 |
| work_keys_str_mv | AT pradillomonica plantmeiosismethodsandprotocols AT heckmannstefan plantmeiosismethodsandprotocols |