Correlative light and electron microscopy: 4
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| Format: | Book |
| Language: | English |
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Amsterdam [u.a.]
Elsevier, Acad. Press
2021
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| Edition: | First edition |
| Series: | Methods in cell biology
volume 162 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xix, 430 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128220580 |
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| adam_text | Contents Contributors..............................................................................................................xi Preface to CLEM IV: Broaden the horizon...........................................................xix CHAPTER 1 50 Shades of CLEM: How to choosethe right approach for you........................................................... 1 Nadav Scher and Ori Avinoam 1. What is CLEM ?...............................................................................1 2. The microscopist s dilemma...........................................................2 3. Sample preservation........................................................................5 4. Case studies.....................................................................................6 5. How to choose your shade of CLEM: Closing remarks................8 Acknowledgments................................................................................ 9 References.............................................................................................9 CHAPTER 2 The Histo-CLEM Workflow for tissues of model organisms.................................................................... 13 Ana Laura Sousa, Joana Rodrigues Lotos, Pedro Faísca, and Erin M. Tranfield 1. Introduction....................................................................................14 2. General design of the Histo-CLEM Workflow............................ 16 3. Application example..................................................................... 28 4. Concluding
remarks...................................................................... 34 Acknowledgments...............................................................................34 References...........................................................................................35 CHAPTER 3 Fluorescent platinum nanoclusters ascorrelative light electron microscopy probes.............................. 39 Hugh Tanner, Lorna Hodgson, Judith Mantell, and Paul Verkade 1. Introduction................................................................................... 40 2. CLEM probes................................................................................41 3. Instrumentation, materials, and reagents..................................... 45 4. Methods......................................................................................... 47 5. Generation and validation of the probe........................................49 6. Use of PtNCs.................................................................................57
Contents 7. Discussion and outlook................................................................. 62 Acknowledgments...............................................................................63 References........................................................................................... 64 CHAPTER 4 Refining a correlative lightelectron microscopy workflow using luminescent metalcomplexes............69 Jonathan R. Shewring, Lorna Hodgson, Helen L. Bryant, Per A. Bullough, Julia A. Weinstein, and Paul Verkade 1. Introduction....................................................................................70 2. Transition metal Ir complex 1.......................................................71 3. Instrumentation and materials.......................................................72 4. Methods......................................................................................... 74 5. Results............................................................................................76 6. Discussion...................................................................................... S3 Acknowledgments................................................................................44 References........................................................................................... S4 CHAPTER 5 Sample preparation for energy dispersive X-ray imaging of biological tissues................................ 89 Nicole M. Pirozzi, Jeroen Kuipers, and Ben N.G. Giepmans 1. Introduction......................................................................... 2.
Routine chemical fixation of tissues................................... 3. Resin.................................................................................... 4. Contrasting.......................................................................... 5. Grids and sample holder..................................................... 6. Other considerations........................................................... 7. Conclusion........................................................................... 8. Materials.............................................................................. Acknowledgments.................................................................... Author contributions................................................................. References................................................................................. CHAPTER 6 .0(1 4M UH 102 К Ih 10 111 I11 112 113 HPM live μ for a full CLEM workflow....................... 115 Xavier Heiligenstein, Marit de Beer, Jerome Heiligenstein, Frédérique Eyraud, Laurent Manet, Fabrice Schmitt, Edwin Lamers, Joerg Lindenau, Mariska Kea-te Lindert, Jean Salamero, Graça Raposo, Nico Sommerdijk, Martin Belle, and Anat Akiva 1. Introduction..................................................................................... 117 2. High pressure freezing principles..............................................117 3. Designing the HPM live μ.........................................................1 20
Contents 4. Integration of the HPM live μ in a CLEM workflow................ 131 5. A live imaging, cryo-fluorescence, in-resin fluorescence and EM workflow:Validation at each step........................................ 135 6. Perspectives.................................................................................144 Acknowledgments............................................................................ 145 Author contributions.........................................................................145 References........................................................................................ 145 CHAPTER 7 High-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes.......................................................... 151 Robert Kiewisz, Thomas Miiller-Reichert, and Gunar Fabig 1. Introduction................................................................................. 152 2. Methods.......................................................................................154 3. Instrumentation and materials.....................................................164 4. Discussion................................................................................... 165 Acknowledgments............................................................................ 168 References........................................................................................ 168 CHAPTER 8 Qn-section correlative light and electron microscopy of large cellular volumes using STEM
tomography..................................................................... 171 Korbinian Buerger, Kerstin N. Schmidt, Jantına Fokkema, Hans C. Gerritsen, Olga Maier, Uwe de Vries, Yulia Zaytseva, Reinhard Rachel, and Ralph Witzgall 1. Introduction................................................................................. 173 2. Methods.......................................................................................175 3. Instrumentation and materials.................................................... 180 4. Results.........................................................................................183 5. Discussion................................................................................... 196 6. Conclusion.................................................................................. 199 Acknowledgments............................................................................200 References........................................................................................ 200 CHAPTER 9 An accelerated procedure for approaching and imaging of optically branded region of interest in tissue............................................................................... 205 Katlijn Vints, Pieter Baatsen, and Natalia V. Gounko 1. Introduction................................................................................ 206 2. Rationale..................................................................................... 208 3. Methods...................................................................................... 209
viii Contents 4. 5. Instrumentation and materials.......................................................... 217 Discussion and conclusion................................................................2 IS Acknowledgments..................................................................................... 21 u References................................................................................................... 2 in CHAPTER 10 Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm............................................................... 223 Irene Y. Chang, Mohammad Rahman, Adam Harned, Orna Cohen-Fix, and Kedar Narayan 1. Introduction......................................................... Λ note on selecting the right er oprolectant 3. Rationale.............................................................. 4. Methods............................................................... 5. Instrumentation and maleriais........................ 6. Discussion........................................................... Acknowledgments.................................................. շ2s References................................................................ 7 tl CHAPTER 11 Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI 253 2. 22o 2 11 7-Γ 2-lS Vojtěch Pražák, Kay Grünewald, and Rainer Kaułmann 1. Introduction.................................................................................. 2.
Rationale...................................................................................... 3. Materials....................................................................................... 4. 5. Methods......................................................................................... Discussion............................................................... Acknou lodgments............................................................................ References................................................................................................. CHAPTER 12 Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells...................................................... Steffen Klein, Moritz Wachsmuth-Melm, Sophie L. Winter, Androniki Kolovou, and Petr Chlanda 1. Introduction................................................................................. Rationale.. 3. Materials.. 4. Methods... 2. 2 ՜ո շ՜տ
Contents 5. Results and discussion............................................................... 297 Acknowledgments............................................................................300 References........................................................................................301 CHAPTER 13 Super-resolution correlative light-electron microscopy using a click-chemistry approach for studying intracellular trafficking.............................303 Teodora Andrian, Thomas Bakkum, Daphne M. van Elsland, Erik Bos, Abraham J. Koster, Lorenzo Albertazzi, Sander I. van Kasteren, and Silvia Pujáis 1. Introduction................................................................................ 305 2. Methods...................................................................................... 310 3. Instrumentation and materials.................................................... 319 4. Discussion...................................................................................320 Acknowledgments............................................................................324 References........................................................................................325 CHAPTER 14 Step-by-step guide to post-acquisition correlation of confocal and FIB/SEM volumes using Amira software...............................................333 Allon Weiner 1. Introduction................................................................................ 334 2. Precursor techniques.................................................................. 336 3. Software and
hardware.............................................................. 338 4. Step-by-step guide......................................................................338 5. Summary and discussion............................................................347 Acknowledgments........................................................................... 348 References........................................................................................348 CHAPTER 15 Visualization and co-registration of correlative microscopy data with the ImageJ plug-in Correlia...353 Matthias Schmidt, Florens Rohde, and Ulf-Dietrich Braumann 1. Introduction................................................................................ 355 2. Models for image co-registration...............................................358 3. Correlia...................................................................................... 359 4. Two real-life examples.............................................................. 370 5. Summary and conclusions..........................................................385 Acknowledgments........................................................................... 386 References........................................................................................386 IX
x Contents CHAPTER 16 Multimodality imaging beyond CLEM: Showcases of combined in-vivo preclinical imaging and exvivo microscopy to detect murine mural vascular lesions............................................................................. 389 Katharina Keuenhof, Patrick Heimel, LM. Zopf, Martin Räigel, Anna Turyanskaya, Anoop Kavirayani, Susanne Reier, Martin Glösmann, Christian Schöfer, Robert Kralovics, Christina Streli, Wolfgang J. Weninger, Stefan H. Geyer, Paul Slezák, Karin Macfelda, Roland Jäger, Thomas Wanek, and Andreas Walter 1. Introduction...................................................................................... WI 2. Rationale............................................................................................ 3. Methods and results..........................................................................tos 4. Discussion......................................................................................... -ton 5. Conclusion and outlook................................................................... Ill Acknowledgment...................................................................................-11՝ References.............................................................................................. -11 ՝ CHAPTER 17 Correlative multimodal imaging: Building a community........................................................................417 Andreas Walter, Gerard J. Kleywegt, and Paul Verkade 1. Setting the scene.............................................................................. 41X 2. A brief
history.................................................................................. 4 IS 3. Building the community................................................................. 42n 4. Conclusion........................................................................................ 42՜ Acknowledgments.................................................................................42l References..............................................................................................42օ
|
| adam_txt |
Contents Contributors.xi Preface to CLEM IV: Broaden the horizon.xix CHAPTER 1 50 Shades of CLEM: How to choosethe right approach for you. 1 Nadav Scher and Ori Avinoam 1. What is CLEM ?.1 2. The microscopist's dilemma.2 3. Sample preservation.5 4. Case studies.6 5. How to choose your shade of CLEM: Closing remarks.8 Acknowledgments. 9 References.9 CHAPTER 2 The Histo-CLEM Workflow for tissues of model organisms. 13 Ana Laura Sousa, Joana Rodrigues Lotos, Pedro Faísca, and Erin M. Tranfield 1. Introduction.14 2. General design of the Histo-CLEM Workflow. 16 3. Application example. 28 4. Concluding
remarks. 34 Acknowledgments.34 References.35 CHAPTER 3 Fluorescent platinum nanoclusters ascorrelative light electron microscopy probes. 39 Hugh Tanner, Lorna Hodgson, Judith Mantell, and Paul Verkade 1. Introduction. 40 2. CLEM probes.41 3. Instrumentation, materials, and reagents. 45 4. Methods. 47 5. Generation and validation of the probe.49 6. Use of PtNCs.57
Contents 7. Discussion and outlook. 62 Acknowledgments.63 References. 64 CHAPTER 4 Refining a correlative lightelectron microscopy workflow using luminescent metalcomplexes.69 Jonathan R. Shewring, Lorna Hodgson, Helen L. Bryant, Per A. Bullough, Julia A. Weinstein, and Paul Verkade 1. Introduction.70 2. Transition metal Ir complex 1.71 3. Instrumentation and materials.72 4. Methods. 74 5. Results.76 6. Discussion. S3 Acknowledgments.44 References. S4 CHAPTER 5 Sample preparation for energy dispersive X-ray imaging of biological tissues. 89 Nicole M. Pirozzi, Jeroen Kuipers, and Ben N.G. Giepmans 1. Introduction. 2.
Routine chemical fixation of tissues. 3. Resin. 4. Contrasting. 5. Grids and sample holder. 6. Other considerations. 7. Conclusion. 8. Materials. Acknowledgments. Author contributions. References. CHAPTER 6 .0(1 4M UH 102 К Ih 10' 111 I11 112 113 HPM live μ for a full CLEM workflow. 115 Xavier Heiligenstein, Marit de Beer, Jerome Heiligenstein, Frédérique Eyraud, Laurent Manet, Fabrice Schmitt, Edwin Lamers, Joerg Lindenau, Mariska Kea-te Lindert, Jean Salamero, Graça Raposo, Nico Sommerdijk, Martin Belle, and Anat Akiva 1. Introduction. 117 2. High pressure freezing principles.117 3. Designing the HPM live μ.1 20
Contents 4. Integration of the HPM live μ in a CLEM workflow. 131 5. A live imaging, cryo-fluorescence, in-resin fluorescence and EM workflow:Validation at each step. 135 6. Perspectives.144 Acknowledgments. 145 Author contributions.145 References. 145 CHAPTER 7 High-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes. 151 Robert Kiewisz, Thomas Miiller-Reichert, and Gunar Fabig 1. Introduction. 152 2. Methods.154 3. Instrumentation and materials.164 4. Discussion. 165 Acknowledgments. 168 References. 168 CHAPTER 8 Qn-section correlative light and electron microscopy of large cellular volumes using STEM
tomography. 171 Korbinian Buerger, Kerstin N. Schmidt, Jantına Fokkema, Hans C. Gerritsen, Olga Maier, Uwe de Vries, Yulia Zaytseva, Reinhard Rachel, and Ralph Witzgall 1. Introduction. 173 2. Methods.175 3. Instrumentation and materials. 180 4. Results.183 5. Discussion. 196 6. Conclusion. 199 Acknowledgments.200 References. 200 CHAPTER 9 An accelerated procedure for approaching and imaging of optically branded region of interest in tissue. 205 Katlijn Vints, Pieter Baatsen, and Natalia V. Gounko 1. Introduction. 206 2. Rationale. 208 3. Methods. 209
viii Contents 4. 5. Instrumentation and materials. 217 Discussion and conclusion.2 IS Acknowledgments. 21 u References. 2 in CHAPTER 10 Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm. 223 Irene Y. Chang, Mohammad Rahman, Adam Harned, Orna Cohen-Fix, and Kedar Narayan 1. Introduction. Λ note on selecting the right er\oprolectant 3. Rationale. 4. Methods. 5. Instrumentation and maleriais. 6. Discussion. Acknowledgments. շ2s References. 7 tl CHAPTER 11 Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI 253 2. 22o 2 11 7-Γ 2-lS Vojtěch Pražák, Kay Grünewald, and Rainer Kaułmann 1. Introduction. 2.
Rationale. 3. Materials. 4. 5. Methods. Discussion. Acknou lodgments. References. CHAPTER 12 Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Steffen Klein, Moritz Wachsmuth-Melm, Sophie L. Winter, Androniki Kolovou, and Petr Chlanda 1. Introduction. Rationale. 3. Materials. 4. Methods. 2. 2 ՜ո շ՜տ
Contents 5. Results and discussion. 297 Acknowledgments.300 References.301 CHAPTER 13 Super-resolution correlative light-electron microscopy using a click-chemistry approach for studying intracellular trafficking.303 Teodora Andrian, Thomas Bakkum, Daphne M. van Elsland, Erik Bos, Abraham J. Koster, Lorenzo Albertazzi, Sander I. van Kasteren, and Silvia Pujáis 1. Introduction. 305 2. Methods. 310 3. Instrumentation and materials. 319 4. Discussion.320 Acknowledgments.324 References.325 CHAPTER 14 Step-by-step guide to post-acquisition correlation of confocal and FIB/SEM volumes using Amira software.333 Allon Weiner 1. Introduction. 334 2. Precursor techniques. 336 3. Software and
hardware. 338 4. Step-by-step guide.338 5. Summary and discussion.347 Acknowledgments. 348 References.348 CHAPTER 15 Visualization and co-registration of correlative microscopy data with the ImageJ plug-in Correlia.353 Matthias Schmidt, Florens Rohde, and Ulf-Dietrich Braumann 1. Introduction. 355 2. Models for image co-registration.358 3. Correlia. 359 4. Two real-life examples. 370 5. Summary and conclusions.385 Acknowledgments. 386 References.386 IX
x Contents CHAPTER 16 Multimodality imaging beyond CLEM: Showcases of combined in-vivo preclinical imaging and exvivo microscopy to detect murine mural vascular lesions. 389 Katharina Keuenhof, Patrick Heimel, LM. Zopf, Martin Räigel, Anna Turyanskaya, Anoop Kavirayani, Susanne Reier, Martin Glösmann, Christian Schöfer, Robert Kralovics, Christina Streli, Wolfgang J. Weninger, Stefan H. Geyer, Paul Slezák, Karin Macfelda, Roland Jäger, Thomas Wanek, and Andreas Walter 1. Introduction. WI 2. Rationale. 3. Methods and results.tos 4. Discussion. -ton 5. Conclusion and outlook. Ill Acknowledgment.-11՝ References. -11 ՝ CHAPTER 17 Correlative multimodal imaging: Building a community.417 Andreas Walter, Gerard J. Kleywegt, and Paul Verkade 1. Setting the scene. 41X 2. A brief
history. 4 IS 3. Building the community. 42n 4. Conclusion. 42՜ Acknowledgments.42l References.42օ |
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| spelling | Correlative light and electron microscopy 4 ed. by Thomas Müller-Reichert, Paul Verkade First edition Amsterdam [u.a.] Elsevier, Acad. Press 2021 xix, 430 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology volume 162 Methods in cell biology (DE-588)4143413-4 Aufsatzsammlung gnd-content Müller-Reichert, Thomas 1962- Sonstige (DE-588)132464020 oth Verkade, Paul (DE-588)113549391X edt (DE-604)BV042194467 4 Erscheint auch als Online-Ausgabe 978-0-1282-2059-7 Methods in cell biology volume 162 (DE-604)BV002534878 162 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032614005&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Correlative light and electron microscopy Methods in cell biology |
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| title | Correlative light and electron microscopy |
| title_auth | Correlative light and electron microscopy |
| title_exact_search | Correlative light and electron microscopy |
| title_exact_search_txtP | Correlative light and electron microscopy |
| title_full | Correlative light and electron microscopy 4 ed. by Thomas Müller-Reichert, Paul Verkade |
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| title_full_unstemmed | Correlative light and electron microscopy 4 ed. by Thomas Müller-Reichert, Paul Verkade |
| title_short | Correlative light and electron microscopy |
| title_sort | correlative light and electron microscopy |
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