'1968' and the women's liberation movement in Britain:
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2020
|
| Online Access: | Inhaltsverzeichnis |
| ISSN: | 2197-0386 |
Staff View
MARC
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| 941 | |h 64 |j 2020 |s 35-40 | ||
Record in the Search Index
| _version_ | 1804182059813437440 |
|---|---|
| adam_text | CONTENTS
I
I
NTRODUCTION
I
1
L
IFE
AND
GENOME
SIZE
3
2
G
ENOME
ORGANISATION
IN
SPACE
AND
TIME
5
2.1
GENOME
ARCHITECTURE
DURING
THE
CELL
CYCLE
........................
6
2.2
DNA
LOOP
EXTRUSION
BY
SMC
COMPLEXES
...........................
12
2.3
ANATOMY
OF
SMC
COMPLEXES
AND
THEIR
INTERACTION
WITH
DNA
14
2.4
CHROMATIN
RECONSTITUTION
IN
XENOPUS
EGG
EXTRACTS
...........
17
3
T
HIS
WORK
-
AIM
AND
SCOPE
21
II
R
ESULTS
23
4
R
ECONSTITUTION
OF
DNA
LOOP
EXTRUSION
25
4.1
REAL-TIME
IMAGING
OF
IMMOBILIZED
DNA
MOLECULES
....
26
4.1.1
CORRECTION
FOR
DYE-INDUCED
DNA
ELONGATION
....
29
4.1.2
LIVE
IMAGING
OF
CHROMATIN
ASSEMBLY
IN
EGG
EXTRACT
33
4.2
RECONSTITUTION
OF
DNA
LOOP
EXTRUSION
BY
HISTONE
DEPLETION
36
4.3
SUMMARY
...........................................................................
39
5
B
IOPHYSICAL
CHARACTERIZATION
OF
DNA
LOOP
EXTRUSION
41
5.1
QUANTIFICATION
OF
DNA
LOOP
EXTRUSION
SYMMETRY
...........
42
5.1.1
DNA
LOOP
EXTRUSION
IS
NON-SYMMETRIC
IN
METAPHASE
AND
SYMMETRIC
IN
INTERPHASE
................................
46
5.1.2
UNUSUAL
EXTRUSION
SYMMETRIES
IN
A
SMALL
SUB-POPULATION
OF
LOOPS
................................................................
50
5.1.3
LOOP
MOVEMENT
AND
EXTRUSION
SYMMETRIES
ARE
COR
RELATED
........................................................
53
5.2
SIMILAR
RATES
AND
FORCES
IN
INTER-
AND
METAPHASE
.............
57
5.2.1
THE
RATE
OF
LOOP
EXTRUSION
IS
TENSION-DEPENDENT
.
.
61
5.3
SUMMARY
...........................................................................
63
XIV
CONTENTS
6
I
DENTIFICATION
OF
COHESIN
AND
CONDENSIN
AS
NATIVE
DNA
LOOPING
FACTORS
65
6.1
CONDENSIN
EXTRUDES
DNA
LOOPS
IN
METAPHASE
................
66
6.2
COHESIN
EXTRUDES
DNA
LOOPS
IN
INTERPHASE
......................
69
6.3
SUMMARY
............................................................................
71
III
D
ISCUSSION
73
7
S
UMMARY
75
8
D
ISCUSSION
79
8.1
PHENOTYPES
OF
MULTIPLE
LOOP
EXTRUSION
EVENTS
................
79
8.2
LOOP
EXTRUSION
PROPERTIES
AND
BIOLOGICAL
FUNCTION
...........
82
8.2.1
LOOP
EXTRUSION
SYMMETRY
AND
CHROMOSOME
ORGANI
SATION
...........................................................
83
8.2.2
LOOP
EXTRUSION
RATES,
STALL
FORCES
AND
GENOME
DY
NAMICS
........................................................
85
8.2.3
CELL
CYCLE-SPECIFIC
REGULATION
OF
DNA
LOOP
EXTRUSION
88
8.3
OUTLOOK:
BUILDING
UP
COMPLEXITY
......................................
90
IV
M
ETHODS
93
9
E
XPERIMENTAL
M
ETHODS
95
9.1
SINGLE
DNA
MOLECULE
SLIDE
PREPARATION
...........................
95
9.1.1
LAMBDA-DNA
BIOTINYLATION
...................................
95
9.1.2
COVER
SLIP
PASSIVATION
...........................................
96
9.1.3
DNA-CHANNEL
PREPARATION
......................................
97
9.2
ANTIBODY
PRODUCTION
.........................................................
97
9.2.1
ANTIGEN-SELECTION,
RABBIT
IMMUNIZATION
AND
ANTI
BODY
PURIFICATION
........................................
98
9.2.2
FLUORESCENT
ANTIBODY
LABELLING
..............................
98
9.3
XENOPUS
LAEVIS
EGG
EXTRACT
.................................................
99
9.3.1
ETHICS
STATEMENT
...................................................
99
9.3.2
CYTOPLASMIC
EGG
EXTRACT
PREPARATION
......................
99
9.3.3
IMMUNO-DEPLETION
OF
HISTONES,
COHESIN
AND
CONDENSIN
100
9.3.4
WESTERN
BLOTS
OF
DEPLETED
EXTRACT
...........................
101
9.3.5
IMAGING
OF
DNA
LOOP
EXTRUSION
...........................
101
9.3.6
IMMUNO-STAINING
OF
DNA
MOLECULES
...................
102
9.3.7
DNA
STRETCHING
EXPERIMENTS
.................................
103
9.4
DATA
ANALYSIS
....................................................................
103
9.4.1
DATA
PRE-PROCESSING
..............................................
103
9.4.2
DNA
STRAND
SEGMENTATION
......................................
104
V
A
PPENDIX
A
A
PPENDIX
B
IBLIOGRAPHY
CONTENTS
XV
105
107
113
|
| adam_txt |
CONTENTS
I
I
NTRODUCTION
I
1
L
IFE
AND
GENOME
SIZE
3
2
G
ENOME
ORGANISATION
IN
SPACE
AND
TIME
5
2.1
GENOME
ARCHITECTURE
DURING
THE
CELL
CYCLE
.
6
2.2
DNA
LOOP
EXTRUSION
BY
SMC
COMPLEXES
.
12
2.3
ANATOMY
OF
SMC
COMPLEXES
AND
THEIR
INTERACTION
WITH
DNA
14
2.4
CHROMATIN
RECONSTITUTION
IN
XENOPUS
EGG
EXTRACTS
.
17
3
T
HIS
WORK
-
AIM
AND
SCOPE
21
II
R
ESULTS
23
4
R
ECONSTITUTION
OF
DNA
LOOP
EXTRUSION
25
4.1
REAL-TIME
IMAGING
OF
IMMOBILIZED
DNA
MOLECULES
.
26
4.1.1
CORRECTION
FOR
DYE-INDUCED
DNA
ELONGATION
.
29
4.1.2
LIVE
IMAGING
OF
CHROMATIN
ASSEMBLY
IN
EGG
EXTRACT
33
4.2
RECONSTITUTION
OF
DNA
LOOP
EXTRUSION
BY
HISTONE
DEPLETION
36
4.3
SUMMARY
.
39
5
B
IOPHYSICAL
CHARACTERIZATION
OF
DNA
LOOP
EXTRUSION
41
5.1
QUANTIFICATION
OF
DNA
LOOP
EXTRUSION
SYMMETRY
.
42
5.1.1
DNA
LOOP
EXTRUSION
IS
NON-SYMMETRIC
IN
METAPHASE
AND
SYMMETRIC
IN
INTERPHASE
.
46
5.1.2
UNUSUAL
EXTRUSION
SYMMETRIES
IN
A
SMALL
SUB-POPULATION
OF
LOOPS
.
50
5.1.3
LOOP
MOVEMENT
AND
EXTRUSION
SYMMETRIES
ARE
COR
RELATED
.
53
5.2
SIMILAR
RATES
AND
FORCES
IN
INTER-
AND
METAPHASE
.
57
5.2.1
THE
RATE
OF
LOOP
EXTRUSION
IS
TENSION-DEPENDENT
.
.
61
5.3
SUMMARY
.
63
XIV
CONTENTS
6
I
DENTIFICATION
OF
COHESIN
AND
CONDENSIN
AS
NATIVE
DNA
LOOPING
FACTORS
65
6.1
CONDENSIN
EXTRUDES
DNA
LOOPS
IN
METAPHASE
.
66
6.2
COHESIN
EXTRUDES
DNA
LOOPS
IN
INTERPHASE
.
69
6.3
SUMMARY
.
71
III
D
ISCUSSION
73
7
S
UMMARY
75
8
D
ISCUSSION
79
8.1
PHENOTYPES
OF
MULTIPLE
LOOP
EXTRUSION
EVENTS
.
79
8.2
LOOP
EXTRUSION
PROPERTIES
AND
BIOLOGICAL
FUNCTION
.
82
8.2.1
LOOP
EXTRUSION
SYMMETRY
AND
CHROMOSOME
ORGANI
SATION
.
83
8.2.2
LOOP
EXTRUSION
RATES,
STALL
FORCES
AND
GENOME
DY
NAMICS
.
85
8.2.3
CELL
CYCLE-SPECIFIC
REGULATION
OF
DNA
LOOP
EXTRUSION
88
8.3
OUTLOOK:
BUILDING
UP
COMPLEXITY
.
90
IV
M
ETHODS
93
9
E
XPERIMENTAL
M
ETHODS
95
9.1
SINGLE
DNA
MOLECULE
SLIDE
PREPARATION
.
95
9.1.1
LAMBDA-DNA
BIOTINYLATION
.
95
9.1.2
COVER
SLIP
PASSIVATION
.
96
9.1.3
DNA-CHANNEL
PREPARATION
.
97
9.2
ANTIBODY
PRODUCTION
.
97
9.2.1
ANTIGEN-SELECTION,
RABBIT
IMMUNIZATION
AND
ANTI
BODY
PURIFICATION
.
98
9.2.2
FLUORESCENT
ANTIBODY
LABELLING
.
98
9.3
XENOPUS
LAEVIS
EGG
EXTRACT
.
99
9.3.1
ETHICS
STATEMENT
.
99
9.3.2
CYTOPLASMIC
EGG
EXTRACT
PREPARATION
.
99
9.3.3
IMMUNO-DEPLETION
OF
HISTONES,
COHESIN
AND
CONDENSIN
100
9.3.4
WESTERN
BLOTS
OF
DEPLETED
EXTRACT
.
101
9.3.5
IMAGING
OF
DNA
LOOP
EXTRUSION
.
101
9.3.6
IMMUNO-STAINING
OF
DNA
MOLECULES
.
102
9.3.7
DNA
STRETCHING
EXPERIMENTS
.
103
9.4
DATA
ANALYSIS
.
103
9.4.1
DATA
PRE-PROCESSING
.
103
9.4.2
DNA
STRAND
SEGMENTATION
.
104
V
A
PPENDIX
A
A
PPENDIX
B
IBLIOGRAPHY
CONTENTS
XV
105
107
113 |
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| spelling | Nava, Mica Verfasser (DE-588)172559693 aut '1968' and the women's liberation movement in Britain Mica Nava 2020 txt rdacontent n rdamedia nc rdacarrier number:64 year:2020 pages:35-40 Moving the social Essen, 2020 Heft 64 (2020), Seite 35-40 (DE-604)BV041251610 2197-0386 (DE-600)2731668-3 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032470253&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nava, Mica '1968' and the women's liberation movement in Britain |
| title | '1968' and the women's liberation movement in Britain |
| title_auth | '1968' and the women's liberation movement in Britain |
| title_exact_search | '1968' and the women's liberation movement in Britain |
| title_exact_search_txtP | '1968' and the women's liberation movement in Britain |
| title_full | '1968' and the women's liberation movement in Britain Mica Nava |
| title_fullStr | '1968' and the women's liberation movement in Britain Mica Nava |
| title_full_unstemmed | '1968' and the women's liberation movement in Britain Mica Nava |
| title_short | '1968' and the women's liberation movement in Britain |
| title_sort | 1968 and the women s liberation movement in britain |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032470253&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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