Homologous Recombination Methods and Protocols - Aguilera, Andrés; Carreira, Aura (Springer US, 2021)
Detection of DNA Double Strand Breaks by γ-H2AX Immunodetection -- END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells -- Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting -- Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe -- Quantifying DNA End Resection in Human Cells -- Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure-Forming Repeats -- Biochemical analysis of D-Loop Extension and DNA Strand Displacement Synthesis -- DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing -- Monitoring Homologous Recombination Activity in Human Cells -- Inter-Homolog Homologous Recombination in Mouse Embryonic Stem Cells -- Branch Migration Activity of Rad54 Protein -- Holliday Junction Resolution -- Identification and Analysis of Different Types of UFBs --
- Intrachromosomal Recombination in Yeast -- Genome-Wide Analysis of Mitotic Recombination in Budding Yeast -- Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis -- DNA Double-Strand Break Induced Gene Amplification in Yeast -- Measuring Chromosome Pairing during Homologous Recombination in Yeast -- Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes -- Detection of DSBs in C. elegans Meiosis -- Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae -- Investigation of Break-Induced Replication in Yeast -- Measurement of Homologous Recombination at Stalled Mammalian Replication Forks -- Super Resolution Imaging of Homologous Recombination Repair at Collapsed Replication Forks -- The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bi-Dimensional Gel Electrophoresis -- The Sister Chromatid Exchange Assay in Human Cells -- Analysis of Recombination at Yeast Telomeres --
- Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae -- Analyzing Homologous Recombination at a Genome Wide Level -- CRISPR/Cas9 Induced Breaks in Heterochromatin, Visualized by Immunofluorescence -- In Vivo Binding of Recombination Proteins of Non-DSB DNA Lesions to Replication Forks -- Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells -- In Vitro Characterization of Sumoylation of HR Proteins -- High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products -- Fluorescence Microscopy for Analysis of Relocalization of Structure-Specific Endonucleases -- Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-Invasion-Induced Rearrangements in S. cerevisiae