Plant Chemical Genomics Methods and Protocols - Hicks, Glenn R.; Zhang, Chunhua (Springer US, 2021)
Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated -- Signaling Events in Plants -- Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor -- Whole Seedling Based Chemical Genetic Screens in Arabidopsis -- Identification of Type iii Secretion Inhibitors for Plant Disease Management -- Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture -- In Vivo Chemical Genomics with Random Cyclized Peptides -- Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence -- The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling -- Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening -- A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling -- Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening -- Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors -- Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach -- Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays -- Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule -- Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein -- Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule