Plant Chemical Genomics Methods and Protocols - Hicks, Glenn R.; Zhang, Chunhua (Springer US, 2021)
Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated – Signaling Events in Plants – Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor – Whole Seedling Based Chemical Genetic Screens in Arabidopsis – Identification of Type iii Secretion Inhibitors for Plant Disease Management – Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture – In Vivo Chemical Genomics with Random Cyclized Peptides – Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence – The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling – Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening – A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling – Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening – Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors – Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach – Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays – Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule – Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein – Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule