Crop Breeding: Genetic Improvement Methods
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications,...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2264 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field |
| Beschreibung: | Methods of Development of Biparental Mapping Populations in Horticultural Crops -- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits -- Using Metabolomics to Assist Plant Breeding -- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications -- High Resolution Melting Analysis as a Tool for Plant Species Authentication -- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods -- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome -- Association Mapping in Plants -- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) -- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis -- RNA Interference (RNAi) in Tomato Crop Reseserch -- Protoplast Based Method for Genome Editing in Tetraploid Potato -- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria -- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant -- Assembly of TALEN and mTALE-Act for Plant Genome Engineering -- Genome Editing to Achieve the Crop Ideotype in Tomato -- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging -- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis |
| Beschreibung: | 1 Online-Ressource (XI, 280 Seiten) Illustrationen |
| ISBN: | 9781071612019 |
| DOI: | 10.1007/978-1-0716-1201-9 |
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| 520 | |a This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field | ||
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| spelling | Crop Breeding Genetic Improvement Methods edited by Pasquale Tripodi 1st edition New York, NY Springer US 2021 1 Online-Ressource (XI, 280 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2264 Methods of Development of Biparental Mapping Populations in Horticultural Crops -- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits -- Using Metabolomics to Assist Plant Breeding -- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications -- High Resolution Melting Analysis as a Tool for Plant Species Authentication -- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods -- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome -- Association Mapping in Plants -- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) -- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis -- RNA Interference (RNAi) in Tomato Crop Reseserch -- Protoplast Based Method for Genome Editing in Tetraploid Potato -- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria -- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant -- Assembly of TALEN and mTALE-Act for Plant Genome Engineering -- Genome Editing to Achieve the Crop Ideotype in Tomato -- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging -- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field Biomedical engineering Agriculture Tripodi, Pasquale edt Erscheint auch als Druck-Ausgabe 9781071612002 Erscheint auch als Druck-Ausgabe 9781071612026 Erscheint auch als Druck-Ausgabe 9781071612033 https://doi.org/10.1007/978-1-0716-1201-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Crop Breeding Genetic Improvement Methods Biomedical engineering Agriculture |
| title | Crop Breeding Genetic Improvement Methods |
| title_auth | Crop Breeding Genetic Improvement Methods |
| title_exact_search | Crop Breeding Genetic Improvement Methods |
| title_exact_search_txtP | Crop Breeding Genetic Improvement Methods |
| title_full | Crop Breeding Genetic Improvement Methods edited by Pasquale Tripodi |
| title_fullStr | Crop Breeding Genetic Improvement Methods edited by Pasquale Tripodi |
| title_full_unstemmed | Crop Breeding Genetic Improvement Methods edited by Pasquale Tripodi |
| title_short | Crop Breeding |
| title_sort | crop breeding genetic improvement methods |
| title_sub | Genetic Improvement Methods |
| topic | Biomedical engineering Agriculture |
| topic_facet | Biomedical engineering Agriculture |
| url | https://doi.org/10.1007/978-1-0716-1201-9 |
| work_keys_str_mv | AT tripodipasquale cropbreedinggeneticimprovementmethods |