Bibliographische Detailangaben

Weitere Verfasser:	Adamski, Jerzy ca. 20./21. Jh (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	London, United Kingdom ; San Diego, CA, United States ; Cambridge, MA, United States ; Kidlington, Oxford, United Kingdom Academic Press, an imprint of Elsevier 2020
Schlagworte:	Metabolites
Online-Zugang:	TUM01
Beschreibung:	Intro -- Metabolomics for Biomedical Research -- Copyright -- Contents -- Contributors -- Preface -- Chapter 1: Introduction to metabolomics -- 1. What is metabolomics? -- 2. Flow chart of metabolomic research -- 2.1. Scientific question -- 2.2. Study design: General aspects -- 2.3. Study design: Pilot study and power calculation -- 2.4. Study design: Sample identity -- 2.5. Study design: Preanalytics -- 2.6. Study design: Sample matrix -- 2.7. Study design: Confounders -- 2.8. Study design: Time schedule -- 2.9. Study design: Randomization -- 2.10. Study design: Budgeting and resources 2.11. Study design: Contingency plan -- 2.12. Study design: Legal aspects -- 2.13. Study design: Governance -- 2.14. Study design: Replication -- 2.15. Analytics -- 2.16. Data validation -- 2.17. Data release -- 3. Future trends -- 3.1. Standardization -- 3.2. Coverage and speed -- 3.3. Accessibility -- 3.4. Mechanisms of disease -- 3.5. Biomarkers -- Acknowledgments -- References -- Chapter 2: Confounders in metabolomics -- 1. Introduction to the confounder concept -- 2. Important confounders in metabolomics -- 2.1. Genetic background and ethnicity -- 2.2. Sex -- 2.3. Age -- 2.4. Nutrition 3. Body mass index -- 3.1. Physical activity -- 3.2. Alcohol -- 3.3. Smoking -- 3.4. Stress -- 3.5. Circadian rhythm -- 3.6. Hormonal status -- 3.7. Medication -- 3.8. Lifestyle -- 4. Disease -- 5. Consequences for study design -- 6. Future trends -- References -- Chapter 3: Pre-analytics in biomedical metabolomics -- 1. Introduction -- 2. Principles to be considered before the collection of body fluids for biomedical metabolomics -- 2.1. Patients instructions and sophisticated questionaries can avoid outliers -- Points to be considered before sample collection Suggestions of choice for additional points in a study questionnaire -- 2.1.1. Control groups: Undiagnosed, asymptomatic diseases of apparently healthy subjects can bias metabolomics findings -- 2.2. Sample collectors for blood and urine in biomedical metabolomics studies -- 2.2.1. Blood collection tubes are potential sources of chemical noise disturbing metabolomics analysis -- 2.2.2. Additives in plasma blood collection tubes which can be used and which should be avoided -- 2.2.3. Additives in serum blood collection tubes may lead to chemical noise 2.2.4. Dried blood spot metabolomics: Stability of the detected mixture of metabolites from erythrocytes, leukocytes, thr ... -- 2.2.5. Urine cups and containers: Pretests are imperative to avoid analytical problems -- 2.3. Animal studies: Some pre-analytical characteristics are different from human biomedical metabolomics -- 3. Collection, handling, and storage of body fluids for metabolomics investigations -- 3.1. Blood -- 3.1.1. Handling of whole blood can greatly affect the sample quality and the metabolome of plasma and serum -- Plasma: Time and temperature until plasma separation is crucial. - Serum: The coagulation process must be tightly controlled
Beschreibung:	1 Online-Ressource (268 Seiten)
ISBN:	0128127856 9780128127858