Quantitative real-time PCR: methods and protocols
This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethic...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2020]
|
| Ausgabe: | Second edition |
| Schriftenreihe: | Methods in molecular biology
2065 |
| Schlagworte: | |
| Zusammenfassung: | This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study |
| Beschreibung: | This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study 1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers; Alessandro Raso and Roberto Biassoni; ; 2. Parameters for Successful PCR Primer Design; Stephen A. Bustin, Reinhold Mueller, and Tania Nolan; ; 3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing; Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl; ; 4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation; Valentin Vautrot and Isabelle Behm-Ansmant; ; 5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR; Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati; ; 6. - Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR); Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, and Alice Gomes Fernandes Monteiro; ; 7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR); Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, and Belén Rodelas; ; 8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine; Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani; ; 9. A General Protocol for Accurate Gene Expression Analysis in Plants; Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek; ; 10. Gene Expression Analysis in Bacteria by RT-qPCR; Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco; ; 11. - Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR; Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani; ; 12. Molecular Monitoring of Chronic Myeloid Leukemia; Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad; ; 13. Normalization in Human Gliomas Tissue; Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Márcia Rosangela Wink; ; 14. qPCR Applications for the Determination of the Biological Age; Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi; ; 15. QuantStudio™ 12K Flex OpenArray System as a Tool for High-Throughput Genotyping and Gene Expression Analysis; Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato; ; 16. - Digital PCR and the QuantStudio™ 3D Digital PCR System; Marion Laig, Christie Fekete, and Nivedita Majumdar |
| Beschreibung: | xiii, 235 Seiten Illustrationen, Diagramme 485 grams |
| ISBN: | 9781493998357 |
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| 500 | |a This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study | ||
| 500 | |a 1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers; Alessandro Raso and Roberto Biassoni; ; 2. Parameters for Successful PCR Primer Design; Stephen A. Bustin, Reinhold Mueller, and Tania Nolan; ; 3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing; Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl; ; 4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation; Valentin Vautrot and Isabelle Behm-Ansmant; ; 5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR; Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati; ; 6. | ||
| 500 | |a - Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR); Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, and Alice Gomes Fernandes Monteiro; ; 7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR); Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, and Belén Rodelas; ; 8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine; Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani; ; 9. A General Protocol for Accurate Gene Expression Analysis in Plants; Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek; ; 10. Gene Expression Analysis in Bacteria by RT-qPCR; Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco; ; 11. | ||
| 500 | |a - Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR; Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani; ; 12. Molecular Monitoring of Chronic Myeloid Leukemia; Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad; ; 13. Normalization in Human Gliomas Tissue; Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Márcia Rosangela Wink; ; 14. qPCR Applications for the Determination of the Biological Age; Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi; ; 15. QuantStudio™ 12K Flex OpenArray System as a Tool for High-Throughput Genotyping and Gene Expression Analysis; Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato; ; 16. | ||
| 500 | |a - Digital PCR and the QuantStudio™ 3D Digital PCR System; Marion Laig, Christie Fekete, and Nivedita Majumdar | ||
| 520 | |a This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study | ||
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| spelling | Quantitative real-time PCR methods and protocols edited by Roberto Biassoni, Alessandro Raso Second edition New York, NY Humana Press [2020] xiii, 235 Seiten Illustrationen, Diagramme 485 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2065 Springer protocols This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study 1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers; Alessandro Raso and Roberto Biassoni; ; 2. Parameters for Successful PCR Primer Design; Stephen A. Bustin, Reinhold Mueller, and Tania Nolan; ; 3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing; Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl; ; 4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation; Valentin Vautrot and Isabelle Behm-Ansmant; ; 5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR; Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati; ; 6. - Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR); Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, and Alice Gomes Fernandes Monteiro; ; 7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR); Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, and Belén Rodelas; ; 8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine; Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani; ; 9. A General Protocol for Accurate Gene Expression Analysis in Plants; Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek; ; 10. Gene Expression Analysis in Bacteria by RT-qPCR; Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco; ; 11. - Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR; Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani; ; 12. Molecular Monitoring of Chronic Myeloid Leukemia; Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad; ; 13. Normalization in Human Gliomas Tissue; Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Márcia Rosangela Wink; ; 14. qPCR Applications for the Determination of the Biological Age; Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi; ; 15. QuantStudio™ 12K Flex OpenArray System as a Tool for High-Throughput Genotyping and Gene Expression Analysis; Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato; ; 16. - Digital PCR and the QuantStudio™ 3D Digital PCR System; Marion Laig, Christie Fekete, and Nivedita Majumdar bicssc bisacsh Genetics Human genetics Life sciences Real time quantitative PCR (DE-588)7544651-0 gnd rswk-swf Hardcover, Softcover / Biologie/Genetik, Gentechnik (DE-588)4143413-4 Aufsatzsammlung gnd-content Real time quantitative PCR (DE-588)7544651-0 s DE-604 Biassoni, Roberto (DE-588)1198136812 edt Raso, Alessandro (DE-588)1198137150 edt Erscheint auch als Online-Ausgabe 978-1-4939-9833-3 Methods in molecular biology 2065 (DE-604)BV035362695 2065 |
| spellingShingle | Quantitative real-time PCR methods and protocols Methods in molecular biology bicssc bisacsh Genetics Human genetics Life sciences Real time quantitative PCR (DE-588)7544651-0 gnd |
| subject_GND | (DE-588)7544651-0 (DE-588)4143413-4 |
| title | Quantitative real-time PCR methods and protocols |
| title_auth | Quantitative real-time PCR methods and protocols |
| title_exact_search | Quantitative real-time PCR methods and protocols |
| title_exact_search_txtP | Quantitative real-time PCR methods and protocols |
| title_full | Quantitative real-time PCR methods and protocols edited by Roberto Biassoni, Alessandro Raso |
| title_fullStr | Quantitative real-time PCR methods and protocols edited by Roberto Biassoni, Alessandro Raso |
| title_full_unstemmed | Quantitative real-time PCR methods and protocols edited by Roberto Biassoni, Alessandro Raso |
| title_short | Quantitative real-time PCR |
| title_sort | quantitative real time pcr methods and protocols |
| title_sub | methods and protocols |
| topic | bicssc bisacsh Genetics Human genetics Life sciences Real time quantitative PCR (DE-588)7544651-0 gnd |
| topic_facet | bicssc bisacsh Genetics Human genetics Life sciences Real time quantitative PCR Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT biassoniroberto quantitativerealtimepcrmethodsandprotocols AT rasoalessandro quantitativerealtimepcrmethodsandprotocols |