Quantum dots: applications in biology

This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots’ (QD) properties, to evaluate their quantum yields, important features about preparative processes and char...

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Weitere Verfasser: Fontes, Adriana (HerausgeberIn)
Format: Buch
Sprache:English
Veröffentlicht: New York, NY Humana Press [2020]
Ausgabe:Third edition
Schriftenreihe:Methods in molecular bology 2135
Schlagworte:
Zusammenfassung:This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots’ (QD) properties, to evaluate their quantum yields, important features about preparative processes and characterizations of QDs, methods related to QDs for live cell applications, and the versatility of QDs in the bioanalytical and biosensing field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Beschreibung:Part I: Tutorials; 1. Multivariate Optimization: A Powerful Tool for the Systematic Control of Quantum Dots Properties; Isabelle Moraes Amorim Viegas, Giovannia Araujo de Lima Pereira, and Claudete Fernandes Pereira; ; 2. Absolute and Relative Methods for Fluorescence Quantum Yield Evaluation of Quantum Dots; Renato E. de Araujo and Christian T. Dominguez; ; Part II: Preparative, Processes, and Characterizations; 3. Preparation and Analysis of Quantum Dots – Applications of Capillary Electrophoresis; Karel Klepárník and Pavlína Modlitbová; ; 4. Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopy; Diogo B. Almeida and André A. de Thomaz; ; 5. Encapsulating Quantum Dots in Lipid-PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjugation ; Alexander M. Saeboe, Joshua C. Kays, and Allison M. Dennis; ; 6. Quantitative Analysis of Single Quantum Dot Trajectories; Oleg Kovtun, Lucas B. Thal, Travis C.
- Josephs, and Sandra J. Rosenthal; ; 7. Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy to Prepare, Low-Cost Zwitterionic Polymer; Margaret Chern, Chloe Grazon, and Allison M. Dennis; ; Part III: Cell Applications; 8. Dextran-Functionalized Quantum Dot-Immunoconjugates for Cellular Imaging; Kelly Rees, Melissa Massey, Michael V. Tran, and W. Russ Algar; ; 9. Labeling Neuronal Proteins with Quantum Dots for Single Molecule Imaging; Lucas B. Thal, Oleg Kovtun, and Sandra J. Rosenthal; ; 10. Quantitative Chemical Delivery of Quantum Dots into the Cytosol of Cells; Joseph L. Watson, Alessandra Stangherlin, and Emmanuel Derivery; ; 11. Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and Magnetic Nanoparticles; Galina Nifontova, Fernanda Ramos-Gomes, Frauke Alves, Igor Nabiev, and Alyona Sukhanova; ; 12. Resazurin-based Assay to Evaluate Cell Viability after Quantum Dot Interaction; Maria Isabela A.
- Pereira, Camila Aparecida P. Monteiro, Weslley Felix de Oliveira,; Beate S. Santos, Adriana Fontes, and Paulo E. Cabral Filho; ; Part IV: Bioanalysis; 13. Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot–Based Lab-on-Bead System; Tatiana Tsoy, Alexander Karaulov, Igor Nabiev, and Alyona Sukhanova; ; 14. CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing; Yi-Fan Ruan, Xiao-Mei Shi, Hai-Yan Wang, Wei-Wei Zhao, Jing-Juan Xu, and Hong-Yuan Chen; ; 15. Quantum Dots-based Lateral Flow Test Strip for Glutathione Detection; Zhaohui Li, Juanzu Liu, Lin Zhang, and Jianjun Li ; ; 16. Comparative Advantages and Limitations of Quantum Dots in the Protein Array Applications; Nizar Ayadi, Florian Lafont, Cathy Charlier, Houda Benhelli-Mokrani, Pavel Sokolov, Alyona Sukhanova, Fabrice Fleury, and Igor Nabiev; ; 17.
- Development of Optical Sensors Based on Quantum Dots Using Molecularly Imprinted Polymers for Determination of Prilocaine; Nafiseh Kazemifard, Ali A. Ensafi, Zeinab Saberi; 18. Detection of Tetanus Antibody Applying a Cu-Zn-In-S/ZnS Quantum Dots-based Lateral Flow Immunoassay ; Hong-Min Meng, Juan Chen, Lingbo Qu, and Zhaohui Li; ; 19. Quantum Dots for Assessment of Reactive Oxygen Species Accumulation during ; Chemotherapy and Radiotherapy; Kimal Honour Djam, Bong Han Lee, Sindhuja Suresh, and Andrew Edet Ekpenyong;
Beschreibung:xi, 308 Seiten Illustrationen, Diagramme 801 grams
ISBN:9781071604625