RNA interference and CRISPR technologies: technical advances and new therapeutic opportunities
This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2020]
|
| Schriftenreihe: | Methods in molecular biology
2115 |
| Schlagworte: | |
| Zusammenfassung: | This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine |
| Beschreibung: | Preface…; Table of Contents…; Contributing Authors…; 1. RNA and CRISPR Interferences: Past, Present and Future Perspectives ; Mouldy Sioud; 2. Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents; Kim A. Lennox and Mark A. Behlke; 3. Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo; Ivan V. Chernikov, Mariya I. Meschaninova, and Elena L. Chernolovskaya; 4. Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy; Seung Koo Lee, Benedict Law, and Ching-Hsuan Tung; 5. PAMAM Dendrimers as a Delivery System for Small Interfering RNA; Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, and Michael R. Hamblin; 6. Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo; Duo Zhang, Heedoo Lee, and Yang Jin; 7. Optimized siRNA Delivery into Primary Immune Cells using Electroporation ; Mouldy Sioud ; 8. - Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference; Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, and Xinjing Tang; 9. Exploring 5’-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency ; Anne Mobergslien and Mouldy Sioud; 10. In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector; Njabulo Mnyandu, Patrick Arbuthnot, and Mohube Betty Maepa; 11. Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors; Fiona T. van den Berg, Abdullah Ely, and Patrick Arbuthnot; 12. MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302; Shi-Lung Lin, Jack S. Chen, and Shao-Yao Ying; 13. Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers; Jaroslav Juracek and Ondrej Slaby; 14. - Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference ; Stein Sæbøe-Larssen and Mouldy Sioud; 15. Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference ; Mouldy Sioud; 16. Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy; Per Ole Iversen and Mouldy Sioud; 17. Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing ; Karin Zins and Dietmar Abraham; 18. Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency; Nicholas Paul Casey, Jon Amund Kyte, and Hiroshi Fujiwara; 19. CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity; Kasidet Hiranniramol, Yuhao Chen, and Xiaowei Wang; 20. CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly; Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A. Mustard, and Megan Keniry; 21. - Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task; Sergey V. Prykhozhij, Anna Cordeiro-Santanach, Lucia Caceres, and Jason N. Berman; 22. Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted? ; Sébastien Walchli and Mouldy Sioud; 23. Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy; Xingying Zhang, Chen Chen, Wen Sun, and Haoyi Wang; 24. CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells ; Kanut Laoharawee, Matthew J. Johnson, and Branden S Moriarity ; 25. Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology; Baoyan Bai, June Helen Myklebust, and Sébastien Wälchli; 26. Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation; Perihan Mir, Malte Ritter, Karl Welte, Julia Skokowa, and Maksim Klimiankou; 27. - CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation; Benjamin Dannenmann, Masoud Nasri, Karl Welte, and Julia Skokowa |
| Beschreibung: | xvi, 486 seiten Illustrationen, Diagramme 1155 grams |
| ISBN: | 9781071602898 |
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| 500 | |a - Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference; Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, and Xinjing Tang; 9. Exploring 5’-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency ; Anne Mobergslien and Mouldy Sioud; 10. In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector; Njabulo Mnyandu, Patrick Arbuthnot, and Mohube Betty Maepa; 11. Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors; Fiona T. van den Berg, Abdullah Ely, and Patrick Arbuthnot; 12. MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302; Shi-Lung Lin, Jack S. Chen, and Shao-Yao Ying; 13. Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers; Jaroslav Juracek and Ondrej Slaby; 14. | ||
| 500 | |a - Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference ; Stein Sæbøe-Larssen and Mouldy Sioud; 15. Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference ; Mouldy Sioud; 16. Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy; Per Ole Iversen and Mouldy Sioud; 17. Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing ; Karin Zins and Dietmar Abraham; 18. Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency; Nicholas Paul Casey, Jon Amund Kyte, and Hiroshi Fujiwara; 19. CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity; Kasidet Hiranniramol, Yuhao Chen, and Xiaowei Wang; 20. CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly; Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A. Mustard, and Megan Keniry; 21. | ||
| 500 | |a - Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task; Sergey V. Prykhozhij, Anna Cordeiro-Santanach, Lucia Caceres, and Jason N. Berman; 22. Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted? ; Sébastien Walchli and Mouldy Sioud; 23. Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy; Xingying Zhang, Chen Chen, Wen Sun, and Haoyi Wang; 24. CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells ; Kanut Laoharawee, Matthew J. Johnson, and Branden S Moriarity ; 25. Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology; Baoyan Bai, June Helen Myklebust, and Sébastien Wälchli; 26. Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation; Perihan Mir, Malte Ritter, Karl Welte, Julia Skokowa, and Maksim Klimiankou; 27. | ||
| 500 | |a - CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation; Benjamin Dannenmann, Masoud Nasri, Karl Welte, and Julia Skokowa | ||
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| spelling | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities edited by Mouldy Sioud New York, NY Humana Press [2020] xvi, 486 seiten Illustrationen, Diagramme 1155 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2115 Springer Protocols Preface…; Table of Contents…; Contributing Authors…; 1. RNA and CRISPR Interferences: Past, Present and Future Perspectives ; Mouldy Sioud; 2. Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents; Kim A. Lennox and Mark A. Behlke; 3. Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo; Ivan V. Chernikov, Mariya I. Meschaninova, and Elena L. Chernolovskaya; 4. Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy; Seung Koo Lee, Benedict Law, and Ching-Hsuan Tung; 5. PAMAM Dendrimers as a Delivery System for Small Interfering RNA; Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, and Michael R. Hamblin; 6. Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo; Duo Zhang, Heedoo Lee, and Yang Jin; 7. Optimized siRNA Delivery into Primary Immune Cells using Electroporation ; Mouldy Sioud ; 8. - Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference; Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, and Xinjing Tang; 9. Exploring 5’-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency ; Anne Mobergslien and Mouldy Sioud; 10. In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector; Njabulo Mnyandu, Patrick Arbuthnot, and Mohube Betty Maepa; 11. Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors; Fiona T. van den Berg, Abdullah Ely, and Patrick Arbuthnot; 12. MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302; Shi-Lung Lin, Jack S. Chen, and Shao-Yao Ying; 13. Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers; Jaroslav Juracek and Ondrej Slaby; 14. - Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference ; Stein Sæbøe-Larssen and Mouldy Sioud; 15. Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference ; Mouldy Sioud; 16. Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy; Per Ole Iversen and Mouldy Sioud; 17. Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing ; Karin Zins and Dietmar Abraham; 18. Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency; Nicholas Paul Casey, Jon Amund Kyte, and Hiroshi Fujiwara; 19. CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity; Kasidet Hiranniramol, Yuhao Chen, and Xiaowei Wang; 20. CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly; Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A. Mustard, and Megan Keniry; 21. - Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task; Sergey V. Prykhozhij, Anna Cordeiro-Santanach, Lucia Caceres, and Jason N. Berman; 22. Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted? ; Sébastien Walchli and Mouldy Sioud; 23. Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy; Xingying Zhang, Chen Chen, Wen Sun, and Haoyi Wang; 24. CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells ; Kanut Laoharawee, Matthew J. Johnson, and Branden S Moriarity ; 25. Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology; Baoyan Bai, June Helen Myklebust, and Sébastien Wälchli; 26. Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation; Perihan Mir, Malte Ritter, Karl Welte, Julia Skokowa, and Maksim Klimiankou; 27. - CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation; Benjamin Dannenmann, Masoud Nasri, Karl Welte, and Julia Skokowa This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine Gene expression Genetics Medicine CRISPR/Cas-Methode (DE-588)1116100770 gnd rswk-swf RNS-Interferenz (DE-588)4802706-6 gnd rswk-swf Klinische Fächer (DE-588)4143413-4 Aufsatzsammlung gnd-content CRISPR/Cas-Methode (DE-588)1116100770 s RNS-Interferenz (DE-588)4802706-6 s DE-604 Sioud, Mouldy (DE-588)1210178265 edt Erscheint auch als Online-Ausgabe 978-1-0716-0290-4 Methods in molecular biology 2115 (DE-604)BV035362695 2115 |
| spellingShingle | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities Methods in molecular biology Gene expression Genetics Medicine CRISPR/Cas-Methode (DE-588)1116100770 gnd RNS-Interferenz (DE-588)4802706-6 gnd |
| subject_GND | (DE-588)1116100770 (DE-588)4802706-6 (DE-588)4143413-4 |
| title | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities |
| title_auth | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities |
| title_exact_search | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities |
| title_exact_search_txtP | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities |
| title_full | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities edited by Mouldy Sioud |
| title_fullStr | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities edited by Mouldy Sioud |
| title_full_unstemmed | RNA interference and CRISPR technologies technical advances and new therapeutic opportunities edited by Mouldy Sioud |
| title_short | RNA interference and CRISPR technologies |
| title_sort | rna interference and crispr technologies technical advances and new therapeutic opportunities |
| title_sub | technical advances and new therapeutic opportunities |
| topic | Gene expression Genetics Medicine CRISPR/Cas-Methode (DE-588)1116100770 gnd RNS-Interferenz (DE-588)4802706-6 gnd |
| topic_facet | Gene expression Genetics Medicine CRISPR/Cas-Methode RNS-Interferenz Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT sioudmouldy rnainterferenceandcrisprtechnologiestechnicaladvancesandnewtherapeuticopportunities |