Preparative chromatography:
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| Weitere Verfasser: | , , |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim, Germany
Wiley-VCH
[2020]
|
| Ausgabe: | Third edition |
| Schlagworte: | |
| Online-Zugang: | http://www.wiley-vch.de/publish/dt/books/ISBN978-3-527-34486-4/ Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | xxviii, 620 Seiten Illustrationen, Diagramme |
| ISBN: | 9783527344864 3527344861 |
Internformat
MARC
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| 020 | |a 9783527344864 |c Festeinband : circa EUR 199.00 (DE) (freier Preis) |9 978-3-527-34486-4 | ||
| 020 | |a 3527344861 |9 3-527-34486-1 | ||
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| 084 | |a CIT 060 |2 stub | ||
| 084 | |a CHE 232 |2 stub | ||
| 084 | |a CHE 230 |2 stub | ||
| 130 | 0 | |a Preparative chromatography of fine chemicals and pharmaceutical agents | |
| 245 | 1 | 0 | |a Preparative chromatography |c edited by Henner Schmidt-Traub, Michael Schulte, Andreas Seidel-Morgenstern |
| 250 | |a Third edition | ||
| 264 | 1 | |a Weinheim, Germany |b Wiley-VCH |c [2020] | |
| 300 | |a xxviii, 620 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 0 | 7 | |a Chromatographie |0 (DE-588)4010153-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Präparative Chromatographie |0 (DE-588)4435196-3 |2 gnd |9 rswk-swf |
| 653 | |a Analytical Chemistry | ||
| 653 | |a Analytische Chemie | ||
| 653 | |a Chemical Engineering | ||
| 653 | |a Chemie | ||
| 653 | |a Chemische Verfahrenstechnik | ||
| 653 | |a Chemistry | ||
| 653 | |a Chromatographie | ||
| 653 | |a Chromatographie / Trennverfahren | ||
| 653 | |a Chromatography / Separation Techniques | ||
| 653 | |a Industrial Chemistry | ||
| 653 | |a Pharmaceutical & Medicinal Chemistry | ||
| 653 | |a Pharmazeutische u. Medizinische Chemie | ||
| 653 | |a Technische u. Industrielle Chemie | ||
| 653 | |a CG00: Allg. Chemische Verfahrenstechnik | ||
| 653 | |a CH10: Analytische Chemie | ||
| 653 | |a CH11: Chromatographie / Trennverfahren | ||
| 653 | |a CH30: Technische u. Industrielle Chemie | ||
| 653 | |a CH60: Pharmazeutische u. Medizinische Chemie | ||
| 655 | 7 | |0 (DE-588)4143413-49783030157289 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Präparative Chromatographie |0 (DE-588)4435196-3 |D s |
| 689 | 0 | 1 | |a Chromatographie |0 (DE-588)4010153-8 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Schmidt-Traub, Henner |d 1940-2020 |0 (DE-588)10689496X |4 edt | |
| 700 | 1 | |a Schulte, Michael |0 (DE-588)1027783600 |4 edt | |
| 700 | 1 | |a Seidel-Morgenstern, Andreas |d 1956- |0 (DE-588)114946582 |4 edt | |
| 710 | 2 | |a Wiley-VCH |0 (DE-588)16179388-5 |4 pbl | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, PDF |z 978-3-527-81631-6 |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, EPUB |z 978-3-527-81633-0 |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-3-527-81634-7 |
| 856 | 4 | 2 | |m X:MVB |u http://www.wiley-vch.de/publish/dt/books/ISBN978-3-527-34486-4/ |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032227156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032227156 | ||
Datensatz im Suchindex
| _version_ | 1804181630238064640 |
|---|---|
| adam_text | CONTENTS
PREFACE
XV
ABOUT
THE
EDITORS
XVII
LIST
OF
ABBREVIATIONS
XIX
NOTATION
XXIII
1
INTRODUCTION
1
HENNER
SCHMIDT-TRAUB
AND
REINHARD
DITZ
1.1
CHROMATOGRAPHY,
DEVELOPMENT,
AND
FUTURE
TRENDS
1
1.2
FOCUS
OF
THE
BOOK
4
1.3
SUGGESTIONS
ON
HOW
TO
READ
THIS
BOOK
4
REFERENCES
6
2
FUNDAMENTALS
AND
GENERAL
TERMINOLOGY
9
ANDREAS
SEIDEL-MORGENSTERN
2.1
PRINCIPLES
AND
FEATURES
OF
CHROMATOGRAPHY
9
2.2
ANALYSIS
AND
DESCRIPTION
OF
CHROMATOGRAMS
13
2.2.1
VOIDAGE
AND
POROSITY
13
2.2.2
RETENTION
TIMES
AND
CAPACITY
FACTORS
16
2.2.3
EFFICIENCY
OF
CHROMATOGRAPHIC
SEPARATIONS
17
2.2.4
RESOLUTION
20
2.2.5
PRESSURE
DROP
23
2.3
MASS
TRANSFER
AND
FLUID
DYNAMICS
25
2.3.1
PRINCIPLES
OF
MASS
TRANSFER
25
2.3.2
FLUID
DISTRIBUTION
IN
THE
COLUMN
27
2.3.3
PACKING
NONIDEALITIES
28
2.3.4
EXTRA-COLUMN
EFFECTS
29
2.4
EQUILIBRIUM
THERMODYNAMICS
29
2.4.1
DEFINITION
OF
ISOTHERMS
29
2.4.2
MODELS
OF
ISOTHERMS
31
2.4.2.1
SINGLE-COMPONENT
ISOTHERMS
31
2.4.2.2
MULTICOMPONENT
ISOTHERMS
BASED
ON
THE
LANGMUIR
MODEL
33
2.4.2.3
COMPETITIVE
ISOTHERMS
BASED
ON
THE
IDEAL
ADSORBED
SOLUTION
THEORY
34
2.4.2.4
STERIC
MASS
ACTION
ISOTHERMS
37
VI
CONTENTS
2.4.3
2.5
2.5.1
2.5.2
RELATION
BETWEEN
ISOTHERMS
AND
BAND
SHAPES
38
COLUMN
OVERLOADING
AND
OPERATING
MODES
44
OVERLOADING
STRATEGIES
44
BEYOND
ISOCRATIC
BATCH
ELUTION
45
REFERENCES
46
3
STATIONARY
PHASES
49
MICHAEL
SCHULTE
3.1
3.2
3.2.1
3.2.2
3.2.3
SURVEY
OF
PACKINGS
AND
STATIONARY
PHASES
49
INORGANIC
SORBENTS
SO
ACTIVATED
CARBONS
50
SYNTHETIC
ZEOLITES
54
POROUS
OXIDES:
SILICA,
ACTIVATED
ALUMINA,
TITANIA,
ZIRCONIA,
AND
MAGNESIA
54
3.2.4
3.2.4.1
3.2.4.2
3.2.5
3.2.6
3.2.6.1
3.2.6.2
3.2.6.3
3.2.6.4
3.2.6.5
3.2.6.6
3.2.6.7
3.2.6.8
3.2.6.9
SILICA
55
SURFACE
CHEMISTRY
57
MASS
LOADABILITY
59
DIATOMACEOUS
EARTH
59
REVERSED
PHASE
SILICAS
60
SILANIZATION
OF
THE
SILICA
SURFACE
60
SILANIZATION
60
STARTING
SILANES
61
PARENT
POROUS
SILICA
61
REACTION
AND
REACTION
CONDITIONS
62
ENDCAPPING
62
CHROMATOGRAPHIC
CHARACTERIZATION
OF
REVERSED
PHASE
SILICAS
63
CHROMATOGRAPHIC
PERFORMANCE
63
HYDROPHOBIC
PROPERTIES
RETENTION
FACTOR
(AMOUNT
OF
ORGANIC
SOLVENT
FOR
ELUTION),
SELECTIVITY
65
3.2.6.10
3.2.6.11
3.2.6.12
3.2.6.13
3.2.7
3.2.8
3.2.9
3.2.9.1
3.2.9.2
3.2.10
3.3
3.3.1
3.3.2
3.3.3
3.3.4
3.3.4.1
3.3.5
3.3.6
SHAPE
SELECTIVITY
65
SILANOL
ACTIVITY
67
PURITY
68
IMPROVED
PH
STABILITY
SILICA
68
ALUMINUM
OXIDE
69
TITANIUM
DIOXIDE
70
OTHER
OXIDES
71
MAGNESIUM
OXIDE
71
ZIRCONIUM
DIOXIDE
71
POROUS
GLASSES
72
CROSS-LINKED
ORGANIC
POLYMERS
73
GENERAL
ASPECTS
74
HYDROPHOBIC
POLYMER
STATIONARY
PHASES
77
HYDROPHILIC
POLYMER
STATIONARY
PHASES
78
ION
EXCHANGE
(IEX)
79
OPTIMIZATION
OF
ION-EXCHANGE
RESINS
81
MIXED
MODE
88
HYDROXYAPATITE
88
CONTENTS
VII
3.3.7
DESIGNED
ADSORBENTS
91
3.3.7.1
PROTEIN
A
AFFINITY
SORBENTS
91
3.3.7.2
OTHER
IGG
RECEPTOR
PROTEINS:
PROTEIN
G
AND
PROTEIN
L
96
3.3.7.3
SORBENTS
FOR
DERIVATIZED/TAGGED
COMPOUNDS:
IMMOBILIZED
METAL
AFFINITY
CHROMATOGRAPHY
(IMAC)
96
3.3.7.4
OTHER
TAG-BASED
AFFINITY
SORBENTS
101
3.3.8
CUSTOMIZED
ADSORBENTS
102
3.3.8.1
LOW
MOLECULAR
WEIGHT
LIGANDS
105
33.8.2
NATURAL
POLYMERS
(PROTEINS,
POLYNUCLEOTIDES)
108
3.3.83
ARTIFICIAL
POLYMERS
111
3.4
ADVECTIVE
CHROMATOGRAPHIC
MATERIALS
111
3.4.1
ADSORPTIVE
MEMBRANES
AND
GRAFTED-POLYMER
MEMBRANES
114
3.4.2
ADSORPTIVE
NONWOVENS
115
3.4.3
FIBER/PARTICLE
COMPOSITES
117
3.4.4
AREA-ENHANCED
FIBERS
117
3.4.5
MONOLITH
118
3.4.6
CHROMATOGRAPHIC
MATERIALS
FOR
LARGER
MOLECULES
121
3.5
CHIRAL
STATIONARY
PHASES
121
3.5.1
CELLULOSE
AND
AMYLOSE-BASED
CSP
122
3.5.2
ANTIBIOTIC
CSP
128
3.5.3
CYCLOFRUCTAN-BASED
CSP
128
3.5.4
SYNTHETIC
POLYMERS
128
3.5.5
TARGETED
SELECTOR
DESIGN
130
3.5.6
FURTHER
DEVELOPMENTS
132
3.6
PROPERTIES
OF
PACKINGS
AND
THEIR
RELEVANCE
TO
CHROMATOGRAPHIC
PERFORMANCE
132
3.6.1
CHEMICAL
AND
PHYSICAL
BULK
PROPERTIES
132
3.6.2
MORPHOLOGY
133
3.6.3
PARTICULATE
ADSORBENTS:
PARTICLE
SIZE
AND
SIZE
DISTRIBUTION
133
3.6.4
PORE
TEXTURE
134
3.6.5
PORE
STRUCTURAL
PARAMETERS
137
3.6.6
COMPARATIVE
RATING
OF
COLUMNS
137
3.7
SORBENT
MAINTENANCE
AND
REGENERATION
138
3.7.1
CLEANING
IN
PLACE
(CIP)
138
3.7.2
CIP
FOR
IEX
140
3.7.3
CIP
OF
PROTEIN
A
SORBENTS
140
3.7.4
CONDITIONING
OF
SILICA
SURFACES
143
3.7.5
SANITIZATION
IN
PLACE
(SIP)
145
3.7.6
COLUMN
AND
ADSORBENT
STORAGE
145
REFERENCES
146
4
SELECTION
OF
CHROMATOGRAPHIC
SYSTEMS
159
MICHAEL
SCHULTE
4.1
DEFINITION
OF
THE
TASK
164
4.2
MOBILE
PHASES
FOR
LIQUID
CHROMATOGRAPHY
167
4.2.1
STABILITY
168
4.2.2
SAFETY
CONCERNS
172
VIII
CONTENTS
4.2.3
OPERATING
CONDITIONS
172
4.2.4
AQUEOUS
BUFFER
SYSTEMS
176
4.3
ADSORBENT
AND
PHASE
SYSTEMS
178
4.3.1
CHOICE
OF
PHASE
SYSTEM
DEPENDENT
ON
SOLUBILITY
178
4.3.2
IMPROVING
LOADABILITY
FOR
POOR
SOLUBILITIES
180
4.3.3
DEPENDENCY
OF
SOLUBILITY
ON
SAMPLE
PURITY
183
4.3.4
GENERIC
GRADIENTS
FOR
FAST
SEPARATIONS
184
4.4
CRITERIA
FOR
CHOOSING
NORMAL
PHASE
SYSTEMS
184
4.4.1
RETENTION
IN
NP
SYSTEMS
186
4.4.2
SOLVENT
STRENGTH
IN
LIQUID-SOLID
CHROMATOGRAPHY
188
4.4.3
PILOT
TECHNIQUE
THIN-LAYER
CHROMATOGRAPHY
USING
THE
PRISMA
MODEL
190
4.4.3.1
STEP
(1):
SOLVENT
STRENGTH
ADJUSTMENT
199
4.4.3.2
STEP
(2):
OPTIMIZATION
OF
SELECTIVITY
199
4.4.3.3
STEP
(3):
FINAL
OPTIMIZATION
OF
THE
SOLVENT
STRENGTH
200
4.4.3.4
STEP
(4):
DETERMINATION
OF
THE
OPTIMUM
MOBILE
PHASE
COMPOSITION
200
4.4.4
STRATEGY
FOR
AN
INDUSTRIAL
PREPARATIVE
CHROMATOGRAPHY
LABORATORY
202
4.4.4.1
STANDARD
GRADIENT
ELUTION
METHOD
ON
SILICA
203
4.4.4.2
SIMPLIFIED
PROCEDURE
204
4.5
CRITERIA
FOR
CHOOSING
REVERSED
PHASE
SYSTEMS
206
4.5.1
RETENTION
AND
SELECTIVITY
IN
RP
SYSTEMS
208
4.5.2
GRADIENT
ELUTION
FOR
SMALL
AMOUNTS
OF
PRODUCT
ON
RP
COLUMNS
212
4.5.3
RIGOROUS
OPTIMIZATION
FOR
ISOCRATIC
RUNS
213
4.5.4
RIGOROUS
OPTIMIZATION
FOR
GRADIENT
RUNS
217
4.5.5
PRACTICAL
RECOMMENDATIONS
222
4.6
CRITERIA
FOR
CHOOSING
CSP
SYSTEMS
223
4.6.1
SUITABILITY
OF
PREPARATIVE
CSP
223
4.6.2
DEVELOPMENT
OF
ENANTIOSELECTIVITY
224
4.6.3
OPTIMIZATION
OF
SEPARATION
CONDITIONS
226
4.6.3.1
DETERMINATION
OF
RACEMATE
SOLUBILITY
226
4.6.3.2
SELECTION
OF
ELUTION
ORDER
226
4.6.3.3
OPTIMIZATION
OF
MOBILE/STATIONARY
PHASE
COMPOSITION,
INCLUDING
TEMPERATURE
226
4.6.3.4
DETERMINATION
OF
OPTIMUM
SEPARATION
STEP
227
4.6.4
PRACTICAL
RECOMMENDATIONS
227
4.7
DOWNSTREAM
PROCESSING
OF
MABS
USING
PROTEIN
A
AND
IEX
231
4.8
SIZE-EXCLUSION
CHROMATOGRAPHY
(SEC)
236
4.9
OVERALL
CHROMATOGRAPHIC
SYSTEM
OPTIMIZATION
237
4.9.1
CONFLICTS
DURING
OPTIMIZATION
OF
CHROMATOGRAPHIC
SYSTEMS
237
4.9.2
STATIONARY
PHASE
GRADIENTS
241
REFERENCES
246
5
PROCESS
CONCEPTS
251
MALTE
KASPEREIT
AND
HENNER
SCHMIDT-TRAUB
5.1
DISCONTINUOUS
PROCESSES
252
CONTENTS
IX
5.1.1
ISOCRATIC
OPERATION
252
5.1.2
GRADIENT
CHROMATOGRAPHY
253
5.1.3
CLOSED-LOOP
RECYCLING
CHROMATOGRAPHY
256
5.1.4
STEADY-STATE
RECYCLING
CHROMATOGRAPHY
(SSRC)
258
5.1.5
FLIP-FLOP
CHROMATOGRAPHY
259
5.1.6
CHROMATOGRAPHIC
BATCH
REACTORS
260
5.2
CONTINUOUS
PROCESSES
261
5.2.1
COLUMN
SWITCHING
CHROMATOGRAPHY
262
5.2.2
ANNULAR
CHROMATOGRAPHY
262
5.2.3
MULTIPORT
SWITCHING
VALVE
CHROMATOGRAPHY
(ISEP/CSEP)
263
5.2.4
ISOCRATIC
SIMULATED
MOVING
BED
(SMB)
CHROMATOGRAPHY
264
5.2.5
SMB
CHROMATOGRAPHY
WITH
VARIABLE
PROCESS
CONDITIONS
268
5.2.5.1
VARICOL
269
5.2.5.2
POWERFEED
270
5.2.5.3
PARTIAL-FEED,
PARTIAL-DISCARD,
AND
FRACTIONATION-FEEDBACK
CONCEPTS
271
5.2.5.4
IMPROVED/INTERMITTENT
SMB
(ISMB)
271
5.2.5.5
MODICON
273
5.2.5.6
FF-SMB
273
5.2.6
GRADIENT
SMB
CHROMATOGRAPHY
274
5.2.7
SUPERCRITICAL
FLUID
CHROMATOGRAPHY
(SFC)
275
5.2.7.1
SUPERCRITICAL
BATCH
CHROMATOGRAPHY
276
5.2.7.2
SUPERCRITICAL
SMB
PROCESSES
277
5.2.8
MULTICOMPONENT
SEPARATIONS
277
5.2.9
MULTICOLUMN
SYSTEMS
FOR
BIOSEPARATIONS
278
5.2.9.1
MULTICOLUMN
CAPTURE
CHROMATOGRAPHY
(MCC)
279
5.2.9.2
MULTICOLUMN
COUNTERCURRENT
SOLVENT
GRADIENT
PURIFICATION
(MCSGP)
286
5.2.10
COUNTERCURRENT
CHROMATOGRAPHIC
REACTORS
288
5.2.10.1
SMB
REACTOR
288
5.2.10.2
SMB
REACTORS
WITH
DISTRIBUTED
FUNCTIONALITIES
290
5.3
CHOICE
OF
PROCESS
CONCEPTS
292
5.3.1
SCALE
292
5.3.2
RANGE
OF
K
292
5.3.3
NUMBER
OF
FRACTIONS
293
5.3.4
EXAMPLE
1:
LAB
SCALE;
TWO
FRACTIONS
293
5.3.5
EXAMPLE
2:
LAB
SCALE;
THREE
OR
MORE
FRACTIONS
294
5.3.6
EXAMPLE
3:
PRODUCTION
SCALE;
WIDE
RANGE
OF
K
296
5.3.7
EXAMPLE
4:
PRODUCTION
SCALE;
TWO
MAIN
FRACTIONS
297
5.3.8
EXAMPLE
5:
PRODUCTION
SCALE;
THREE
FRACTIONS
298
5.3.9
EXAMPLE
6:
PRODUCTION
SCALE;
MULTISTAGE
PROCESS
300
REFERENCES
302
6
MODELING
OF
CHROMATOGRAPHIC
PROCESSES
311
ANDREAS
SEIDEL-MORGENSTERN
6.1
INTRODUCTION
311
6.2
MODELS
FOR
SINGLE
CHROMATOGRAPHIC
COLUMNS
311
X
CONTENTS
6.2.1
EQUILIBRIUM
STAGE
MODELS
312
6.2.1.1
DISCONTINUOUS
MODEL
ACCORDING
TO
CRAIG
313
6.2.1.2
CONTINUOUS
MODEL
ACCORDING
TO
MARTIN
AND
SYNGE
315
6.2.2
DERIVATION
OF
CONTINUOUS
MASS
BALANCE
EQUATIONS
316
6.2.2.1
MASS
BALANCE
EQUATIONS
318
6.2.2.2
CONVECTIVE
TRANSPORT
320
6.2.2.3
AXIAL
DISPERSION
320
6.2.2.4
INTRAPARTICLE
DIFFUSION
321
6.2.2.5
MASS
TRANSFER
BETWEEN
PHASES
321
6.2.2.6
FINITE
RATES
OF
ADSORPTION
AND
DESORPTION
322
6.2.2.7
ADSORPTION
EQUILIBRIA
323
6.2.3
EQUILIBRIUM
MODEL
OF
CHROMATOGRAPHY
323
6.2.4
MODELS
WITH
ONE
BAND
BROADENING
EFFECT
329
6.2.4.1
EQUILIBRIUM
DISPERSION
MODEL
329
6.2.4.2
FINITE
ADSORPTION
RATE
MODEL
331
6.2.5
CONTINUOUS
LUMPED
RATE
MODELS
331
6.2.5.1
TRANSPORT
DISPERSION
MODELS
332
6.2.5.2
LUMPED
FINITE
ADSORPTION
RATE
MODEL
333
6.2.6
GENERAL
RATE
MODELS
333
6.2.7
INITIAL
AND
BOUNDARY
CONDITIONS
OF
THE
COLUMN
335
6.2.8
DIMENSIONLESS
MODEL
EQUATIONS
336
6.2.9
COMPARISON
OF
DIFFERENT
MODEL
APPROACHES
338
6.3
INCLUDING
EFFECTS
OUTSIDE
THE
COLUMNS
343
6.3.1
EXPERIMENTAL
SETUP
AND
SIMULATION
FLOW
SHEET
343
6.3.2
MODELING
EXTRA-COLUMN
EQUIPMENT
345
6.3.2.1
INJECTION
SYSTEM
345
6.3.2.2
PIPING
345
63.2.3
DETECTOR
345
6.4
CALCULATION
METHODS
AND
SOFTWARE
346
6.4.1
ANALYTICAL
SOLUTIONS
346
6.4.2
NUMERICAL
SOLUTION
METHODS
346
6.4.2.1
DISCRETIZATION
346
6A.2.2
GENERAL
SOLUTION
PROCEDURE
AND
SOFTWARE
349
REFERENCES
350
7
DETERMINATION
OF
MODEL
PARAMETERS
355
ANDREAS
SEIDEL-MORGENSTERN,
ANDREAS
JUPKE,
AND
HENNER
SCHMIDT-TRAUB
7.1
PARAMETER
CLASSES
FOR
CHROMATOGRAPHIC
SEPARATIONS
355
7.1.1
DESIGN
PARAMETERS
355
7.1.2
OPERATING
PARAMETERS
356
7.1.3
MODEL
PARAMETERS
356
7.2
CONCEPT
TO
DETERMINE
MODEL
PARAMETERS
357
7.3
DETECTORS
AND
PARAMETER
ESTIMATION
359
7.3.1
CALIBRATION
OF
DETECTORS
359
7.3.2
PARAMETER
ESTIMATION
360
7.3.3
EVALUATION
OF
CHROMATOGRAMS
362
7.4
DETERMINATION
OF
PACKING
PARAMETERS
363
CONTENTS
XI
7.4.1
VOID
FRACTION
AND
POROSITY
OF
THE
PACKING
363
7.4.2
AXIAL
DISPERSION
363
7.4.3
PRESSURE
DROP
364
7.5
ADSORPTION
ISOTHERMS
365
7.5.1
DETERMINATION
OF
ADSORPTION
ISOTHERMS
365
7.5.2
ESTIMATION
OF
HENRY
COEFFICIENTS 365
7.5.3
STATIC
ISOTHERM
DETERMINATION
METHODS
370
7.5.3.1
BATCH
METHOD
370
7
.5.3.2
ADSORPTION-DESORPTION
METHOD
370
7.5.3.3
CIRCULATION
METHOD
371
7.5.4
DYNAMIC
METHODS
371
7.5.5
FRONTAL
ANALYSIS
371
7.5.6
ANALYSIS
OF
DISPERSED
FRONTS
378
7.5.7
PEAK
MAXIMUM
METHOD
380
7.5.8
MINOR
DISTURBANCE/PERTURBATION METHOD
380
7.5.9
CURVE
FITTING
OF
THE
CHROMATOGRAM
383
7.5.10
DATA
ANALYSIS
AND
ACCURACY
384
7.6
MASS
TRANSFER
KINETICS
386
7.6.1
CORRELATIONS
386
7.6.2
APPLICATION
OF
METHOD
OF
MOMENTS
388
7.7
PLANT
PARAMETERS
389
7.8
EXPERIMENTAL
VALIDATION
OF
COLUMN
MODELS
AND
MODEL
PARAMETERS
391
7.8.1
BATCH
CHROMATOGRAPHY
391
7.8.2
SIMULATED
MOVING
BED
CHROMATOGRAPHY
394
7.8.2.1
MODEL
FORMULATION
AND
PARAMETERS
394
7.8.2.2
EXPERIMENTAL
VALIDATION
400
REFERENCES
404
8
PROCESS
DESIGN
AND
OPTIMIZATION
409
ANDREAS
JUPKE,
ANDREAS
BISELLI,
MALTE
KASPEREIT,
MARTIN
LEIPNITZ,
AND
HENNER
SCHMIDT-TRAUB
8.1
BASIC
PRINCIPLES
AND
DEFINITIONS
409
8.1.1
PERFORMANCE,
COSTS,
AND
OBJECTIVE
FUNCTIONS
409
8.1.1.1
PERFORMANCE
CRITERIA
410
8.1.1.2
ECONOMIC
CRITERIA
411
8.1.1.3
OBJECTIVE
FUNCTIONS
412
8.1.2
DEGREES
OF
FREEDOM
413
8.1.2.1
CATEGORIES
OF
PARAMETERS
413
8.1.2.2
DIMENSIONLESS
OPERATING
AND
DESIGN
PARAMETERS
414
8.1.3
SCALING
BY
DIMENSIONLESS
PARAMETERS
418
8.1.3.1
INFLUENCE
OF
DIFFERENT
HETP
COEFFICIENTS
FOR
EVERY
COMPONENT
419
8.1.3.2
INFLUENCE
OF
FEED
CONCENTRATION
420
8.1.3.3
EXAMPLES
FOR
A
SINGLE-COLUMN
BATCH
CHROMATOGRAPHY
421
8.1.3.4
EXAMPLES
FOR
SMB
PROCESSES
424
8.2
BATCH
CHROMATOGRAPHY
426
8.2.1
FRACTIONATION
MODE
(CUT
STRATEGY)
426
XII
CONTENTS
8.2.2
DESIGN
AND
OPTIMIZATION
OF
BATCH
CHROMATOGRAPHIC
COLUMNS
427
8.2.2.1
PROCESS
PERFORMANCE
DEPENDING
ON
NUMBER
OF
STAGES
AND
LOADING
FACTOR
427
8.2.2.2
DESIGN
AND
OPTIMIZATION
STRATEGY
432
8.2.2.3
OTHER
STRATEGIES
436
8.3
RECYCLING
CHROMATOGRAPHY
437
8.3.1
DESIGN
OF
STEADY-STATE
RECYCLING
CHROMATOGRAPHY
437
8.3.2
SCALE-UP
OF
CLOSED-LOOP
RECYCLING
CHROMATOGRAPHY
440
8.4
CONVENTIONAL
ISOCRATIC
SMB
CHROMATOGRAPHY
445
8.4.1
CONSIDERATIONS
TO
OPTIMAL
CONCENTRATION
PROFILES
IN
SMB
PROCESS
445
8.4.2
PROCESS
DESIGN
BASED
ON
TMB
MODELS
(SHORTCUT
METHODS)
446
8.4.2.1
TRIANGLE
THEORY
FOR
AN
IDEAL
MODEL
WITH
LINEAR
ISOTHERMS
447
8.4.2.2
TRIANGLE
THEORY
FOR
AN
IDEAL
MODEL
WITH
NONLINEAR
ISOTHERMS
449
8.4.2.3
SHORTCUT
TO
APPLY
THE
TRIANGLE
THEORY
ON
A
SYSTEM
WITH
UNKNOWN
ISOTHERMS
ASSUMING
LANGMUIR
CHARACTER
452
8.4.3
PROCESS
DESIGN
AND
OPTIMIZATION
BASED
ON
RIGOROUS
SMB
MODELS
455
8.4.3.1
ESTIMATION
OF
OPERATING
PARAMETER
456
8.4.3.2
OPTIMIZATION
OF
OPERATING
PARAMETERS
FOR
LINEAR
ISOTHERMS
BASED
ON
PROCESS
UNDERSTANDING
457
8.4.3.3
OPTIMIZATION
OF
OPERATING
PARAMETERS
FOR
NONLINEAR
ISOTHERMS
BASED
ON
PROCESS
UNDERSTANDING
458
8.4.3.4
OPTIMIZATION
OF
DESIGN
PARAMETERS
460
8.5
ISOCRATIC
SMB
CHROMATOGRAPHY
UNDER
VARIABLE
OPERATING
CONDITIONS
465
8.5.1
PERFORMANCE
COMPARISON
OF
VARICOL
AND
CONVENTIONAL
SMB
466
8.5.2
PERFORMANCE
COMPARISON
OF
VARICOL,
POWERFEED,
AND
MODICON
WITH
CONVENTIONAL
SMB
470
8.5.3
PERFORMANCE
TRENDS
APPLYING
SMB
CONCEPTS
UNDER
VARIABLE
OPERATING
CONDITIONS
475
8.6
GRADIENT
SMB
CHROMATOGRAPHY
476
8.6.1
STEP
GRADIENT
476
8.6.2
MULTICOLUMN
SOLVENT
GRADIENT
PURIFICATION
PROCESS
482
8.7
MULTICOLUMN
SYSTEMS
FOR
BIOSEPARATIONS
487
8.7.1
DESIGN
OF
TWIN-COLUMN
CAPTURESMB
488
8.7.2
MODELING
OF
MULTICOLUMN
CAPTURE
PROCESSES
490
REFERENCES
493
9
PROCESS
CONTROL
503
SEBASTIAN
ENGELL
AND
ACHIM
KIENLE
9.1
STANDARD
PROCESS
CONTROL
504
9.2
ADVANCED
PROCESS
CONTROL
504
9.2.1
ONLINE
OPTIMIZATION
OF
BATCH
CHROMATOGRAPHY
505
9.2.2
ADVANCED
CONTROL
OF
SMB
CHROMATOGRAPHY
507
9.2.2.1
PURITY
CONTROL
FOR
SMB
PROCESSES
508
9.2.2.2
DIRECT
OPTIMIZING
CONTROL
OF
SMB
PROCESSES
510
CONTENTS
XIII
9.2.3
ADVANCED
PARAMETER
AND
STATE
ESTIMATION
FOR
SMB
PROCESSES
515
9.2.4
ADAPTIVE
CYCLE-TO-CYCLE
CONTROL
517
9.2.5
CONTROL
OF
COUPLED
SIMULATED
MOVING
BED
PROCESSES
FOR
THE
PRODUCTION
OF
PURE
ENANTIOMERS
519
REFERENCES
521
10
CHROMATOGRAPHY
EQUIPMENT:
ENGINEERING
AND
OPERATION
525
HENNER
SCHMIDT-TRAUB
AND
ARTHUR
SUSANTO
10.1
CHALLENGES
FOR
CONCEPTUAL
PROCESS
DESIGN
525
10.1.1
MAIN
COST
FACTORS
FOR
A
CHROMATOGRAPHIC
SYSTEM
527
10.1.2
CONCEPTUAL
PROCESS
DESIGN
528
10.1.2.1
A
CASE
STUDY:
LARGE-SCALE
BIOTECHNOLOGY
PROJECT
529
10.2
ENGINEERING
CHALLENGES
533
10.2.1
CHALLENGES
REGARDING
SANITARY
DESIGN
535
10.2.2
CHALLENGES
DURING
ACCEPTANCE
TESTS
AND
QUALIFICATIONS
539
10.3
COMMERCIAL
CHROMATOGRAPHY
COLUMNS
540
10.3.1
GENERAL
DESIGN
541
10.3.1.1
MANUALLY
MOVED
PISTON
542
10.3.1.2
ELECTRICALLY
OR
HYDRAULICALLY
MOVED
PISTON
542
10.3.2
HIGH
AND
LOW-PRESSURE
COLUMNS
543
10.3.2.1
CHEMICAL
COMPATIBILITY
544
10.3.2.2
FRIT
DESIGN
546
10.3.2.3
SPECIAL
ASPECTS
OF
BIOSEPARATION
549
10.4
COMMERCIAL
CHROMATOGRAPHIC
SYSTEMS
551
10.4.1
GENERAL
DESIGN
ASPECTS:
HIGH-PRESSURE
AND
LOW-PRESSURE
SYSTEMS
551
10.4.2
MATERIAL
553
10.4.3
BATCH
LOW-PRESSURE
LIQUID
CHROMATOGRAPHIC
(LPLC)
SYSTEMS
553
10.4.3.1
INLETS
553
10.4.3.2
VALVES
TO
CONTROL
FLOW
DIRECTION
555
10.4.3.3
PUMPS
556
10.4.3.4
PUMP
AND
VALVE-BASED
AND
GRADIENT
FORMATION
556
10.4.4
BATCH
HIGH-PRESSURE
LIQUID
CHROMATOGRAPHY
558
10.4.4.1
GENERAL
LAYOUT
558
10.4.4.2
INLETS
AND
OUTLETS
559
10.4.4.3
PUMPS
559
10.4.4.4
VALVES
AND
PIPES
562
10.4.5
CONTINUOUS
SYSTEMS:
SIMULATED
MOVING
BED
563
10.4.5.1
GENERAL
LAYOUT
563
10.4.5.2
A
KEY
CHOICE:
THE
RECYCLING
STRATEGY
565
10.4.5.3
PUMPS,
INLETS,
AND
OUTLETS
566
10.4.5.4
VALVES
AND
PIPING
566
10.4.6
AUXILIARY
SYSTEMS
567
10.4.6.1
SLURRY
PREPARATION
TANK
567
10.4.6.2
SLURRY
PUMPS
AND
PACKING
STATIONS
568
10.4.6.3
CRANES
AND
TRANSPORT
UNITS
568
XIV
CONTENTS
10.4.6.4
FILTER
INTEGRITY
TEST
568
10.4.7
DETECTORS
569
10.5
PACKING
METHODS
571
10.5.1
COLUMN
AND
PACKING
METHODOLOGY
SELECTION
571
10.5.2
SLURRY
PREPARATION
572
10.5.3
COLUMN
PREPARATION
574
10.5.4
FLOW
PACKING
575
10.5.5
DYNAMIC
AXIAL
COMPRESSION
(DAC)
PACKING
577
10.5.6
STALL
PACKING
577
10.5.7
COMBINED
METHOD
(STALL
+
DAC)
578
10.5.8
VACUUM
PACKING
580
10.5.9
VIBRATION
PACKING
581
10.5.10
COLUMN
EQUILIBRATION
582
10.5.11
COLUMN
TESTING
AND
STORAGE
583
10.5.11.1
TEST
SYSTEMS
583
10.5.11.2
HYDRODYNAMIC
PROPERTIES
AND
COLUMN
EFFICIENCY
584
10.5.11.3
COLUMN
AND
ADSORBENT
STORAGE
585
10.6
PROCESS
TROUBLESHOOTING
585
10.6.1
TECHNICAL
FAILURES
586
10.6.2
LOSS
OF
PERFORMANCE
587
10.6.2.1
PRESSURE
INCREASE
587
10.6.2.2
LOSS
OF
COLUMN
EFFICIENCY
590
10.6.2.3
VARIATION
OF
ELUTION
PROFILE
591
10.6.2.4
LOSS
OF
PURITY/YIELD
592
10.6.3
COLUMN
STABILITY
592
10.7
DISPOSABLE
TECHNOLOGY
FOR
BIOSEPARATIONS
593
10.7.1
PREPACKED
COLUMNS
596
10.7.2
MEMBRANE
CHROMATOGRAPHY
597
REFERENCES
599
APPENDIX
A
DATA
OF
TEST
SYSTEMS
601
A.L
EMD53986
601
A.2
TROGER
S
BASE
602
A.
3
GLUCOSE
AND
FRUCTOSE
604
A.4
P PHENETHYL
ACETATE
606
REFERENCES
607
INDEX
609
|
| adam_txt |
CONTENTS
PREFACE
XV
ABOUT
THE
EDITORS
XVII
LIST
OF
ABBREVIATIONS
XIX
NOTATION
XXIII
1
INTRODUCTION
1
HENNER
SCHMIDT-TRAUB
AND
REINHARD
DITZ
1.1
CHROMATOGRAPHY,
DEVELOPMENT,
AND
FUTURE
TRENDS
1
1.2
FOCUS
OF
THE
BOOK
4
1.3
SUGGESTIONS
ON
HOW
TO
READ
THIS
BOOK
4
REFERENCES
6
2
FUNDAMENTALS
AND
GENERAL
TERMINOLOGY
9
ANDREAS
SEIDEL-MORGENSTERN
2.1
PRINCIPLES
AND
FEATURES
OF
CHROMATOGRAPHY
9
2.2
ANALYSIS
AND
DESCRIPTION
OF
CHROMATOGRAMS
13
2.2.1
VOIDAGE
AND
POROSITY
13
2.2.2
RETENTION
TIMES
AND
CAPACITY
FACTORS
16
2.2.3
EFFICIENCY
OF
CHROMATOGRAPHIC
SEPARATIONS
17
2.2.4
RESOLUTION
20
2.2.5
PRESSURE
DROP
23
2.3
MASS
TRANSFER
AND
FLUID
DYNAMICS
25
2.3.1
PRINCIPLES
OF
MASS
TRANSFER
25
2.3.2
FLUID
DISTRIBUTION
IN
THE
COLUMN
27
2.3.3
PACKING
NONIDEALITIES
28
2.3.4
EXTRA-COLUMN
EFFECTS
29
2.4
EQUILIBRIUM
THERMODYNAMICS
29
2.4.1
DEFINITION
OF
ISOTHERMS
29
2.4.2
MODELS
OF
ISOTHERMS
31
2.4.2.1
SINGLE-COMPONENT
ISOTHERMS
31
2.4.2.2
MULTICOMPONENT
ISOTHERMS
BASED
ON
THE
LANGMUIR
MODEL
33
2.4.2.3
COMPETITIVE
ISOTHERMS
BASED
ON
THE
IDEAL
ADSORBED
SOLUTION
THEORY
34
2.4.2.4
STERIC
MASS
ACTION
ISOTHERMS
37
VI
CONTENTS
2.4.3
2.5
2.5.1
2.5.2
RELATION
BETWEEN
ISOTHERMS
AND
BAND
SHAPES
38
COLUMN
OVERLOADING
AND
OPERATING
MODES
44
OVERLOADING
STRATEGIES
44
BEYOND
ISOCRATIC
BATCH
ELUTION
45
REFERENCES
46
3
STATIONARY
PHASES
49
MICHAEL
SCHULTE
3.1
3.2
3.2.1
3.2.2
3.2.3
SURVEY
OF
PACKINGS
AND
STATIONARY
PHASES
49
INORGANIC
SORBENTS
SO
ACTIVATED
CARBONS
50
SYNTHETIC
ZEOLITES
54
POROUS
OXIDES:
SILICA,
ACTIVATED
ALUMINA,
TITANIA,
ZIRCONIA,
AND
MAGNESIA
54
3.2.4
3.2.4.1
3.2.4.2
3.2.5
3.2.6
3.2.6.1
3.2.6.2
3.2.6.3
3.2.6.4
3.2.6.5
3.2.6.6
3.2.6.7
3.2.6.8
3.2.6.9
SILICA
55
SURFACE
CHEMISTRY
57
MASS
LOADABILITY
59
DIATOMACEOUS
EARTH
59
REVERSED
PHASE
SILICAS
60
SILANIZATION
OF
THE
SILICA
SURFACE
60
SILANIZATION
60
STARTING
SILANES
61
PARENT
POROUS
SILICA
61
REACTION
AND
REACTION
CONDITIONS
62
ENDCAPPING
62
CHROMATOGRAPHIC
CHARACTERIZATION
OF
REVERSED
PHASE
SILICAS
63
CHROMATOGRAPHIC
PERFORMANCE
63
HYDROPHOBIC
PROPERTIES
RETENTION
FACTOR
(AMOUNT
OF
ORGANIC
SOLVENT
FOR
ELUTION),
SELECTIVITY
65
3.2.6.10
3.2.6.11
3.2.6.12
3.2.6.13
3.2.7
3.2.8
3.2.9
3.2.9.1
3.2.9.2
3.2.10
3.3
3.3.1
3.3.2
3.3.3
3.3.4
3.3.4.1
3.3.5
3.3.6
SHAPE
SELECTIVITY
65
SILANOL
ACTIVITY
67
PURITY
68
IMPROVED
PH
STABILITY
SILICA
68
ALUMINUM
OXIDE
69
TITANIUM
DIOXIDE
70
OTHER
OXIDES
71
MAGNESIUM
OXIDE
71
ZIRCONIUM
DIOXIDE
71
POROUS
GLASSES
72
CROSS-LINKED
ORGANIC
POLYMERS
73
GENERAL
ASPECTS
74
HYDROPHOBIC
POLYMER
STATIONARY
PHASES
77
HYDROPHILIC
POLYMER
STATIONARY
PHASES
78
ION
EXCHANGE
(IEX)
79
OPTIMIZATION
OF
ION-EXCHANGE
RESINS
81
MIXED
MODE
88
HYDROXYAPATITE
88
CONTENTS
VII
3.3.7
DESIGNED
ADSORBENTS
91
3.3.7.1
PROTEIN
A
AFFINITY
SORBENTS
91
3.3.7.2
OTHER
IGG
RECEPTOR
PROTEINS:
PROTEIN
G
AND
PROTEIN
L
96
3.3.7.3
SORBENTS
FOR
DERIVATIZED/TAGGED
COMPOUNDS:
IMMOBILIZED
METAL
AFFINITY
CHROMATOGRAPHY
(IMAC)
96
3.3.7.4
OTHER
TAG-BASED
AFFINITY
SORBENTS
101
3.3.8
CUSTOMIZED
ADSORBENTS
102
3.3.8.1
LOW
MOLECULAR
WEIGHT
LIGANDS
105
33.8.2
NATURAL
POLYMERS
(PROTEINS,
POLYNUCLEOTIDES)
108
3.3.83
ARTIFICIAL
POLYMERS
111
3.4
ADVECTIVE
CHROMATOGRAPHIC
MATERIALS
111
3.4.1
ADSORPTIVE
MEMBRANES
AND
GRAFTED-POLYMER
MEMBRANES
114
3.4.2
ADSORPTIVE
NONWOVENS
115
3.4.3
FIBER/PARTICLE
COMPOSITES
117
3.4.4
AREA-ENHANCED
FIBERS
117
3.4.5
MONOLITH
118
3.4.6
CHROMATOGRAPHIC
MATERIALS
FOR
LARGER
MOLECULES
121
3.5
CHIRAL
STATIONARY
PHASES
121
3.5.1
CELLULOSE
AND
AMYLOSE-BASED
CSP
122
3.5.2
ANTIBIOTIC
CSP
128
3.5.3
CYCLOFRUCTAN-BASED
CSP
128
3.5.4
SYNTHETIC
POLYMERS
128
3.5.5
TARGETED
SELECTOR
DESIGN
130
3.5.6
FURTHER
DEVELOPMENTS
132
3.6
PROPERTIES
OF
PACKINGS
AND
THEIR
RELEVANCE
TO
CHROMATOGRAPHIC
PERFORMANCE
132
3.6.1
CHEMICAL
AND
PHYSICAL
BULK
PROPERTIES
132
3.6.2
MORPHOLOGY
133
3.6.3
PARTICULATE
ADSORBENTS:
PARTICLE
SIZE
AND
SIZE
DISTRIBUTION
133
3.6.4
PORE
TEXTURE
134
3.6.5
PORE
STRUCTURAL
PARAMETERS
137
3.6.6
COMPARATIVE
RATING
OF
COLUMNS
137
3.7
SORBENT
MAINTENANCE
AND
REGENERATION
138
3.7.1
CLEANING
IN
PLACE
(CIP)
138
3.7.2
CIP
FOR
IEX
140
3.7.3
CIP
OF
PROTEIN
A
SORBENTS
140
3.7.4
CONDITIONING
OF
SILICA
SURFACES
143
3.7.5
SANITIZATION
IN
PLACE
(SIP)
145
3.7.6
COLUMN
AND
ADSORBENT
STORAGE
145
REFERENCES
146
4
SELECTION
OF
CHROMATOGRAPHIC
SYSTEMS
159
MICHAEL
SCHULTE
4.1
DEFINITION
OF
THE
TASK
164
4.2
MOBILE
PHASES
FOR
LIQUID
CHROMATOGRAPHY
167
4.2.1
STABILITY
168
4.2.2
SAFETY
CONCERNS
172
VIII
CONTENTS
4.2.3
OPERATING
CONDITIONS
172
4.2.4
AQUEOUS
BUFFER
SYSTEMS
176
4.3
ADSORBENT
AND
PHASE
SYSTEMS
178
4.3.1
CHOICE
OF
PHASE
SYSTEM
DEPENDENT
ON
SOLUBILITY
178
4.3.2
IMPROVING
LOADABILITY
FOR
POOR
SOLUBILITIES
180
4.3.3
DEPENDENCY
OF
SOLUBILITY
ON
SAMPLE
PURITY
183
4.3.4
GENERIC
GRADIENTS
FOR
FAST
SEPARATIONS
184
4.4
CRITERIA
FOR
CHOOSING
NORMAL
PHASE
SYSTEMS
184
4.4.1
RETENTION
IN
NP
SYSTEMS
186
4.4.2
SOLVENT
STRENGTH
IN
LIQUID-SOLID
CHROMATOGRAPHY
188
4.4.3
PILOT
TECHNIQUE
THIN-LAYER
CHROMATOGRAPHY
USING
THE
PRISMA
MODEL
190
4.4.3.1
STEP
(1):
SOLVENT
STRENGTH
ADJUSTMENT
199
4.4.3.2
STEP
(2):
OPTIMIZATION
OF
SELECTIVITY
199
4.4.3.3
STEP
(3):
FINAL
OPTIMIZATION
OF
THE
SOLVENT
STRENGTH
200
4.4.3.4
STEP
(4):
DETERMINATION
OF
THE
OPTIMUM
MOBILE
PHASE
COMPOSITION
200
4.4.4
STRATEGY
FOR
AN
INDUSTRIAL
PREPARATIVE
CHROMATOGRAPHY
LABORATORY
202
4.4.4.1
STANDARD
GRADIENT
ELUTION
METHOD
ON
SILICA
203
4.4.4.2
SIMPLIFIED
PROCEDURE
204
4.5
CRITERIA
FOR
CHOOSING
REVERSED
PHASE
SYSTEMS
206
4.5.1
RETENTION
AND
SELECTIVITY
IN
RP
SYSTEMS
208
4.5.2
GRADIENT
ELUTION
FOR
SMALL
AMOUNTS
OF
PRODUCT
ON
RP
COLUMNS
212
4.5.3
RIGOROUS
OPTIMIZATION
FOR
ISOCRATIC
RUNS
213
4.5.4
RIGOROUS
OPTIMIZATION
FOR
GRADIENT
RUNS
217
4.5.5
PRACTICAL
RECOMMENDATIONS
222
4.6
CRITERIA
FOR
CHOOSING
CSP
SYSTEMS
223
4.6.1
SUITABILITY
OF
PREPARATIVE
CSP
223
4.6.2
DEVELOPMENT
OF
ENANTIOSELECTIVITY
224
4.6.3
OPTIMIZATION
OF
SEPARATION
CONDITIONS
226
4.6.3.1
DETERMINATION
OF
RACEMATE
SOLUBILITY
226
4.6.3.2
SELECTION
OF
ELUTION
ORDER
226
4.6.3.3
OPTIMIZATION
OF
MOBILE/STATIONARY
PHASE
COMPOSITION,
INCLUDING
TEMPERATURE
226
4.6.3.4
DETERMINATION
OF
OPTIMUM
SEPARATION
STEP
227
4.6.4
PRACTICAL
RECOMMENDATIONS
227
4.7
DOWNSTREAM
PROCESSING
OF
MABS
USING
PROTEIN
A
AND
IEX
231
4.8
SIZE-EXCLUSION
CHROMATOGRAPHY
(SEC)
236
4.9
OVERALL
CHROMATOGRAPHIC
SYSTEM
OPTIMIZATION
237
4.9.1
CONFLICTS
DURING
OPTIMIZATION
OF
CHROMATOGRAPHIC
SYSTEMS
237
4.9.2
STATIONARY
PHASE
GRADIENTS
241
REFERENCES
246
5
PROCESS
CONCEPTS
251
MALTE
KASPEREIT
AND
HENNER
SCHMIDT-TRAUB
5.1
DISCONTINUOUS
PROCESSES
252
CONTENTS
IX
5.1.1
ISOCRATIC
OPERATION
252
5.1.2
GRADIENT
CHROMATOGRAPHY
253
5.1.3
CLOSED-LOOP
RECYCLING
CHROMATOGRAPHY
256
5.1.4
STEADY-STATE
RECYCLING
CHROMATOGRAPHY
(SSRC)
258
5.1.5
FLIP-FLOP
CHROMATOGRAPHY
259
5.1.6
CHROMATOGRAPHIC
BATCH
REACTORS
260
5.2
CONTINUOUS
PROCESSES
261
5.2.1
COLUMN
SWITCHING
CHROMATOGRAPHY
262
5.2.2
ANNULAR
CHROMATOGRAPHY
262
5.2.3
MULTIPORT
SWITCHING
VALVE
CHROMATOGRAPHY
(ISEP/CSEP)
263
5.2.4
ISOCRATIC
SIMULATED
MOVING
BED
(SMB)
CHROMATOGRAPHY
264
5.2.5
SMB
CHROMATOGRAPHY
WITH
VARIABLE
PROCESS
CONDITIONS
268
5.2.5.1
VARICOL
269
5.2.5.2
POWERFEED
270
5.2.5.3
PARTIAL-FEED,
PARTIAL-DISCARD,
AND
FRACTIONATION-FEEDBACK
CONCEPTS
271
5.2.5.4
IMPROVED/INTERMITTENT
SMB
(ISMB)
271
5.2.5.5
MODICON
273
5.2.5.6
FF-SMB
273
5.2.6
GRADIENT
SMB
CHROMATOGRAPHY
274
5.2.7
SUPERCRITICAL
FLUID
CHROMATOGRAPHY
(SFC)
275
5.2.7.1
SUPERCRITICAL
BATCH
CHROMATOGRAPHY
276
5.2.7.2
SUPERCRITICAL
SMB
PROCESSES
277
5.2.8
MULTICOMPONENT
SEPARATIONS
277
5.2.9
MULTICOLUMN
SYSTEMS
FOR
BIOSEPARATIONS
278
5.2.9.1
MULTICOLUMN
CAPTURE
CHROMATOGRAPHY
(MCC)
279
5.2.9.2
MULTICOLUMN
COUNTERCURRENT
SOLVENT
GRADIENT
PURIFICATION
(MCSGP)
286
5.2.10
COUNTERCURRENT
CHROMATOGRAPHIC
REACTORS
288
5.2.10.1
SMB
REACTOR
288
5.2.10.2
SMB
REACTORS
WITH
DISTRIBUTED
FUNCTIONALITIES
290
5.3
CHOICE
OF
PROCESS
CONCEPTS
292
5.3.1
SCALE
292
5.3.2
RANGE
OF
K'
292
5.3.3
NUMBER
OF
FRACTIONS
293
5.3.4
EXAMPLE
1:
LAB
SCALE;
TWO
FRACTIONS
293
5.3.5
EXAMPLE
2:
LAB
SCALE;
THREE
OR
MORE
FRACTIONS
294
5.3.6
EXAMPLE
3:
PRODUCTION
SCALE;
WIDE
RANGE
OF
K'
296
5.3.7
EXAMPLE
4:
PRODUCTION
SCALE;
TWO
MAIN
FRACTIONS
297
5.3.8
EXAMPLE
5:
PRODUCTION
SCALE;
THREE
FRACTIONS
298
5.3.9
EXAMPLE
6:
PRODUCTION
SCALE;
MULTISTAGE
PROCESS
300
REFERENCES
302
6
MODELING
OF
CHROMATOGRAPHIC
PROCESSES
311
ANDREAS
SEIDEL-MORGENSTERN
6.1
INTRODUCTION
311
6.2
MODELS
FOR
SINGLE
CHROMATOGRAPHIC
COLUMNS
311
X
CONTENTS
6.2.1
EQUILIBRIUM
STAGE
MODELS
312
6.2.1.1
DISCONTINUOUS
MODEL
ACCORDING
TO
CRAIG
313
6.2.1.2
CONTINUOUS
MODEL
ACCORDING
TO
MARTIN
AND
SYNGE
315
6.2.2
DERIVATION
OF
CONTINUOUS
MASS
BALANCE
EQUATIONS
316
6.2.2.1
MASS
BALANCE
EQUATIONS
318
6.2.2.2
CONVECTIVE
TRANSPORT
320
6.2.2.3
AXIAL
DISPERSION
320
6.2.2.4
INTRAPARTICLE
DIFFUSION
321
6.2.2.5
MASS
TRANSFER
BETWEEN
PHASES
321
6.2.2.6
FINITE
RATES
OF
ADSORPTION
AND
DESORPTION
322
6.2.2.7
ADSORPTION
EQUILIBRIA
323
6.2.3
EQUILIBRIUM
MODEL
OF
CHROMATOGRAPHY
323
6.2.4
MODELS
WITH
ONE
BAND
BROADENING
EFFECT
329
6.2.4.1
EQUILIBRIUM
DISPERSION
MODEL
329
6.2.4.2
FINITE
ADSORPTION
RATE
MODEL
331
6.2.5
CONTINUOUS
LUMPED
RATE
MODELS
331
6.2.5.1
TRANSPORT
DISPERSION
MODELS
332
6.2.5.2
LUMPED
FINITE
ADSORPTION
RATE
MODEL
333
6.2.6
GENERAL
RATE
MODELS
333
6.2.7
INITIAL
AND
BOUNDARY
CONDITIONS
OF
THE
COLUMN
335
6.2.8
DIMENSIONLESS
MODEL
EQUATIONS
336
6.2.9
COMPARISON
OF
DIFFERENT
MODEL
APPROACHES
338
6.3
INCLUDING
EFFECTS
OUTSIDE
THE
COLUMNS
343
6.3.1
EXPERIMENTAL
SETUP
AND
SIMULATION
FLOW
SHEET
343
6.3.2
MODELING
EXTRA-COLUMN
EQUIPMENT
345
6.3.2.1
INJECTION
SYSTEM
345
6.3.2.2
PIPING
345
63.2.3
DETECTOR
345
6.4
CALCULATION
METHODS
AND
SOFTWARE
346
6.4.1
ANALYTICAL
SOLUTIONS
346
6.4.2
NUMERICAL
SOLUTION
METHODS
346
6.4.2.1
DISCRETIZATION
346
6A.2.2
GENERAL
SOLUTION
PROCEDURE
AND
SOFTWARE
349
REFERENCES
350
7
DETERMINATION
OF
MODEL
PARAMETERS
355
ANDREAS
SEIDEL-MORGENSTERN,
ANDREAS
JUPKE,
AND
HENNER
SCHMIDT-TRAUB
7.1
PARAMETER
CLASSES
FOR
CHROMATOGRAPHIC
SEPARATIONS
355
7.1.1
DESIGN
PARAMETERS
355
7.1.2
OPERATING
PARAMETERS
356
7.1.3
MODEL
PARAMETERS
356
7.2
CONCEPT
TO
DETERMINE
MODEL
PARAMETERS
357
7.3
DETECTORS
AND
PARAMETER
ESTIMATION
359
7.3.1
CALIBRATION
OF
DETECTORS
359
7.3.2
PARAMETER
ESTIMATION
360
7.3.3
EVALUATION
OF
CHROMATOGRAMS
362
7.4
DETERMINATION
OF
PACKING
PARAMETERS
363
CONTENTS
XI
7.4.1
VOID
FRACTION
AND
POROSITY
OF
THE
PACKING
363
7.4.2
AXIAL
DISPERSION
363
7.4.3
PRESSURE
DROP
364
7.5
ADSORPTION
ISOTHERMS
365
7.5.1
DETERMINATION
OF
ADSORPTION
ISOTHERMS
365
7.5.2
ESTIMATION
OF
HENRY
COEFFICIENTS 365
7.5.3
STATIC
ISOTHERM
DETERMINATION
METHODS
370
7.5.3.1
BATCH
METHOD
370
7
.5.3.2
ADSORPTION-DESORPTION
METHOD
370
7.5.3.3
CIRCULATION
METHOD
371
7.5.4
DYNAMIC
METHODS
371
7.5.5
FRONTAL
ANALYSIS
371
7.5.6
ANALYSIS
OF
DISPERSED
FRONTS
378
7.5.7
PEAK
MAXIMUM
METHOD
380
7.5.8
MINOR
DISTURBANCE/PERTURBATION METHOD
380
7.5.9
CURVE
FITTING
OF
THE
CHROMATOGRAM
383
7.5.10
DATA
ANALYSIS
AND
ACCURACY
384
7.6
MASS
TRANSFER
KINETICS
386
7.6.1
CORRELATIONS
386
7.6.2
APPLICATION
OF
METHOD
OF
MOMENTS
388
7.7
PLANT
PARAMETERS
389
7.8
EXPERIMENTAL
VALIDATION
OF
COLUMN
MODELS
AND
MODEL
PARAMETERS
391
7.8.1
BATCH
CHROMATOGRAPHY
391
7.8.2
SIMULATED
MOVING
BED
CHROMATOGRAPHY
394
7.8.2.1
MODEL
FORMULATION
AND
PARAMETERS
394
7.8.2.2
EXPERIMENTAL
VALIDATION
400
REFERENCES
404
8
PROCESS
DESIGN
AND
OPTIMIZATION
409
ANDREAS
JUPKE,
ANDREAS
BISELLI,
MALTE
KASPEREIT,
MARTIN
LEIPNITZ,
AND
HENNER
SCHMIDT-TRAUB
8.1
BASIC
PRINCIPLES
AND
DEFINITIONS
409
8.1.1
PERFORMANCE,
COSTS,
AND
OBJECTIVE
FUNCTIONS
409
8.1.1.1
PERFORMANCE
CRITERIA
410
8.1.1.2
ECONOMIC
CRITERIA
411
8.1.1.3
OBJECTIVE
FUNCTIONS
412
8.1.2
DEGREES
OF
FREEDOM
413
8.1.2.1
CATEGORIES
OF
PARAMETERS
413
8.1.2.2
DIMENSIONLESS
OPERATING
AND
DESIGN
PARAMETERS
414
8.1.3
SCALING
BY
DIMENSIONLESS
PARAMETERS
418
8.1.3.1
INFLUENCE
OF
DIFFERENT
HETP
COEFFICIENTS
FOR
EVERY
COMPONENT
419
8.1.3.2
INFLUENCE
OF
FEED
CONCENTRATION
420
8.1.3.3
EXAMPLES
FOR
A
SINGLE-COLUMN
BATCH
CHROMATOGRAPHY
421
8.1.3.4
EXAMPLES
FOR
SMB
PROCESSES
424
8.2
BATCH
CHROMATOGRAPHY
426
8.2.1
FRACTIONATION
MODE
(CUT
STRATEGY)
426
XII
CONTENTS
8.2.2
DESIGN
AND
OPTIMIZATION
OF
BATCH
CHROMATOGRAPHIC
COLUMNS
427
8.2.2.1
PROCESS
PERFORMANCE
DEPENDING
ON
NUMBER
OF
STAGES
AND
LOADING
FACTOR
427
8.2.2.2
DESIGN
AND
OPTIMIZATION
STRATEGY
432
8.2.2.3
OTHER
STRATEGIES
436
8.3
RECYCLING
CHROMATOGRAPHY
437
8.3.1
DESIGN
OF
STEADY-STATE
RECYCLING
CHROMATOGRAPHY
437
8.3.2
SCALE-UP
OF
CLOSED-LOOP
RECYCLING
CHROMATOGRAPHY
440
8.4
CONVENTIONAL
ISOCRATIC
SMB
CHROMATOGRAPHY
445
8.4.1
CONSIDERATIONS
TO
OPTIMAL
CONCENTRATION
PROFILES
IN
SMB
PROCESS
445
8.4.2
PROCESS
DESIGN
BASED
ON
TMB
MODELS
(SHORTCUT
METHODS)
446
8.4.2.1
TRIANGLE
THEORY
FOR
AN
IDEAL
MODEL
WITH
LINEAR
ISOTHERMS
447
8.4.2.2
TRIANGLE
THEORY
FOR
AN
IDEAL
MODEL
WITH
NONLINEAR
ISOTHERMS
449
8.4.2.3
SHORTCUT
TO
APPLY
THE
TRIANGLE
THEORY
ON
A
SYSTEM
WITH
UNKNOWN
ISOTHERMS
ASSUMING
LANGMUIR
CHARACTER
452
8.4.3
PROCESS
DESIGN
AND
OPTIMIZATION
BASED
ON
RIGOROUS
SMB
MODELS
455
8.4.3.1
ESTIMATION
OF
OPERATING
PARAMETER
456
8.4.3.2
OPTIMIZATION
OF
OPERATING
PARAMETERS
FOR
LINEAR
ISOTHERMS
BASED
ON
PROCESS
UNDERSTANDING
457
8.4.3.3
OPTIMIZATION
OF
OPERATING
PARAMETERS
FOR
NONLINEAR
ISOTHERMS
BASED
ON
PROCESS
UNDERSTANDING
458
8.4.3.4
OPTIMIZATION
OF
DESIGN
PARAMETERS
460
8.5
ISOCRATIC
SMB
CHROMATOGRAPHY
UNDER
VARIABLE
OPERATING
CONDITIONS
465
8.5.1
PERFORMANCE
COMPARISON
OF
VARICOL
AND
CONVENTIONAL
SMB
466
8.5.2
PERFORMANCE
COMPARISON
OF
VARICOL,
POWERFEED,
AND
MODICON
WITH
CONVENTIONAL
SMB
470
8.5.3
PERFORMANCE
TRENDS
APPLYING
SMB
CONCEPTS
UNDER
VARIABLE
OPERATING
CONDITIONS
475
8.6
GRADIENT
SMB
CHROMATOGRAPHY
476
8.6.1
STEP
GRADIENT
476
8.6.2
MULTICOLUMN
SOLVENT
GRADIENT
PURIFICATION
PROCESS
482
8.7
MULTICOLUMN
SYSTEMS
FOR
BIOSEPARATIONS
487
8.7.1
DESIGN
OF
TWIN-COLUMN
CAPTURESMB
488
8.7.2
MODELING
OF
MULTICOLUMN
CAPTURE
PROCESSES
490
REFERENCES
493
9
PROCESS
CONTROL
503
SEBASTIAN
ENGELL
AND
ACHIM
KIENLE
9.1
STANDARD
PROCESS
CONTROL
504
9.2
ADVANCED
PROCESS
CONTROL
504
9.2.1
ONLINE
OPTIMIZATION
OF
BATCH
CHROMATOGRAPHY
505
9.2.2
ADVANCED
CONTROL
OF
SMB
CHROMATOGRAPHY
507
9.2.2.1
PURITY
CONTROL
FOR
SMB
PROCESSES
508
9.2.2.2
DIRECT
OPTIMIZING
CONTROL
OF
SMB
PROCESSES
510
CONTENTS
XIII
9.2.3
ADVANCED
PARAMETER
AND
STATE
ESTIMATION
FOR
SMB
PROCESSES
515
9.2.4
ADAPTIVE
CYCLE-TO-CYCLE
CONTROL
517
9.2.5
CONTROL
OF
COUPLED
SIMULATED
MOVING
BED
PROCESSES
FOR
THE
PRODUCTION
OF
PURE
ENANTIOMERS
519
REFERENCES
521
10
CHROMATOGRAPHY
EQUIPMENT:
ENGINEERING
AND
OPERATION
525
HENNER
SCHMIDT-TRAUB
AND
ARTHUR
SUSANTO
10.1
CHALLENGES
FOR
CONCEPTUAL
PROCESS
DESIGN
525
10.1.1
MAIN
COST
FACTORS
FOR
A
CHROMATOGRAPHIC
SYSTEM
527
10.1.2
CONCEPTUAL
PROCESS
DESIGN
528
10.1.2.1
A
CASE
STUDY:
LARGE-SCALE
BIOTECHNOLOGY
PROJECT
529
10.2
ENGINEERING
CHALLENGES
533
10.2.1
CHALLENGES
REGARDING
SANITARY
DESIGN
535
10.2.2
CHALLENGES
DURING
ACCEPTANCE
TESTS
AND
QUALIFICATIONS
539
10.3
COMMERCIAL
CHROMATOGRAPHY
COLUMNS
540
10.3.1
GENERAL
DESIGN
541
10.3.1.1
MANUALLY
MOVED
PISTON
542
10.3.1.2
ELECTRICALLY
OR
HYDRAULICALLY
MOVED
PISTON
542
10.3.2
HIGH
AND
LOW-PRESSURE
COLUMNS
543
10.3.2.1
CHEMICAL
COMPATIBILITY
544
10.3.2.2
FRIT
DESIGN
546
10.3.2.3
SPECIAL
ASPECTS
OF
BIOSEPARATION
549
10.4
COMMERCIAL
CHROMATOGRAPHIC
SYSTEMS
551
10.4.1
GENERAL
DESIGN
ASPECTS:
HIGH-PRESSURE
AND
LOW-PRESSURE
SYSTEMS
551
10.4.2
MATERIAL
553
10.4.3
BATCH
LOW-PRESSURE
LIQUID
CHROMATOGRAPHIC
(LPLC)
SYSTEMS
553
10.4.3.1
INLETS
553
10.4.3.2
VALVES
TO
CONTROL
FLOW
DIRECTION
555
10.4.3.3
PUMPS
556
10.4.3.4
PUMP
AND
VALVE-BASED
AND
GRADIENT
FORMATION
556
10.4.4
BATCH
HIGH-PRESSURE
LIQUID
CHROMATOGRAPHY
558
10.4.4.1
GENERAL
LAYOUT
558
10.4.4.2
INLETS
AND
OUTLETS
559
10.4.4.3
PUMPS
559
10.4.4.4
VALVES
AND
PIPES
562
10.4.5
CONTINUOUS
SYSTEMS:
SIMULATED
MOVING
BED
563
10.4.5.1
GENERAL
LAYOUT
563
10.4.5.2
A
KEY
CHOICE:
THE
RECYCLING
STRATEGY
565
10.4.5.3
PUMPS,
INLETS,
AND
OUTLETS
566
10.4.5.4
VALVES
AND
PIPING
566
10.4.6
AUXILIARY
SYSTEMS
567
10.4.6.1
SLURRY
PREPARATION
TANK
567
10.4.6.2
SLURRY
PUMPS
AND
PACKING
STATIONS
568
10.4.6.3
CRANES
AND
TRANSPORT
UNITS
568
XIV
CONTENTS
10.4.6.4
FILTER
INTEGRITY
TEST
568
10.4.7
DETECTORS
569
10.5
PACKING
METHODS
571
10.5.1
COLUMN
AND
PACKING
METHODOLOGY
SELECTION
571
10.5.2
SLURRY
PREPARATION
572
10.5.3
COLUMN
PREPARATION
574
10.5.4
FLOW
PACKING
575
10.5.5
DYNAMIC
AXIAL
COMPRESSION
(DAC)
PACKING
577
10.5.6
STALL
PACKING
577
10.5.7
COMBINED
METHOD
(STALL
+
DAC)
578
10.5.8
VACUUM
PACKING
580
10.5.9
VIBRATION
PACKING
581
10.5.10
COLUMN
EQUILIBRATION
582
10.5.11
COLUMN
TESTING
AND
STORAGE
583
10.5.11.1
TEST
SYSTEMS
583
10.5.11.2
HYDRODYNAMIC
PROPERTIES
AND
COLUMN
EFFICIENCY
584
10.5.11.3
COLUMN
AND
ADSORBENT
STORAGE
585
10.6
PROCESS
TROUBLESHOOTING
585
10.6.1
TECHNICAL
FAILURES
586
10.6.2
LOSS
OF
PERFORMANCE
587
10.6.2.1
PRESSURE
INCREASE
587
10.6.2.2
LOSS
OF
COLUMN
EFFICIENCY
590
10.6.2.3
VARIATION
OF
ELUTION
PROFILE
591
10.6.2.4
LOSS
OF
PURITY/YIELD
592
10.6.3
COLUMN
STABILITY
592
10.7
DISPOSABLE
TECHNOLOGY
FOR
BIOSEPARATIONS
593
10.7.1
PREPACKED
COLUMNS
596
10.7.2
MEMBRANE
CHROMATOGRAPHY
597
REFERENCES
599
APPENDIX
A
DATA
OF
TEST
SYSTEMS
601
A.L
EMD53986
601
A.2
TROGER
S
BASE
602
A.
3
GLUCOSE
AND
FRUCTOSE
604
A.4
P'PHENETHYL
ACETATE
606
REFERENCES
607
INDEX
609 |
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| any_adam_object_boolean | 1 |
| author2 | Schmidt-Traub, Henner 1940-2020 Schulte, Michael Seidel-Morgenstern, Andreas 1956- |
| author2_role | edt edt edt |
| author2_variant | h s t hst m s ms a s m asm |
| author_GND | (DE-588)10689496X (DE-588)1027783600 (DE-588)114946582 |
| author_facet | Schmidt-Traub, Henner 1940-2020 Schulte, Michael Seidel-Morgenstern, Andreas 1956- |
| building | Verbundindex |
| bvnumber | BV046818739 |
| classification_rvk | VG 7200 |
| classification_tum | CIT 220 CIT 060 CHE 232 CHE 230 |
| ctrlnum | (OCoLC)1155426974 (DE-599)DNB1198427922 |
| dewey-full | 543.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.8 |
| dewey-search | 543.8 |
| dewey-sort | 3543.8 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Chemie Chemie-Ingenieurwesen |
| discipline_str_mv | Chemie / Pharmazie Chemie Chemie-Ingenieurwesen |
| edition | Third edition |
| format | Book |
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| genre | (DE-588)4143413-49783030157289 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV046818739 |
| illustrated | Illustrated |
| index_date | 2024-07-03T15:01:14Z |
| indexdate | 2024-07-10T08:54:42Z |
| institution | BVB |
| institution_GND | (DE-588)16179388-5 |
| isbn | 9783527344864 3527344861 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032227156 |
| oclc_num | 1155426974 |
| open_access_boolean | |
| owner | DE-20 DE-91G DE-BY-TUM |
| owner_facet | DE-20 DE-91G DE-BY-TUM |
| physical | xxviii, 620 Seiten Illustrationen, Diagramme |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Preparative chromatography of fine chemicals and pharmaceutical agents Preparative chromatography edited by Henner Schmidt-Traub, Michael Schulte, Andreas Seidel-Morgenstern Third edition Weinheim, Germany Wiley-VCH [2020] xxviii, 620 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Chromatographie (DE-588)4010153-8 gnd rswk-swf Präparative Chromatographie (DE-588)4435196-3 gnd rswk-swf Analytical Chemistry Analytische Chemie Chemical Engineering Chemie Chemische Verfahrenstechnik Chemistry Chromatographie Chromatographie / Trennverfahren Chromatography / Separation Techniques Industrial Chemistry Pharmaceutical & Medicinal Chemistry Pharmazeutische u. Medizinische Chemie Technische u. Industrielle Chemie CG00: Allg. Chemische Verfahrenstechnik CH10: Analytische Chemie CH11: Chromatographie / Trennverfahren CH30: Technische u. Industrielle Chemie CH60: Pharmazeutische u. Medizinische Chemie (DE-588)4143413-49783030157289 Aufsatzsammlung gnd-content Präparative Chromatographie (DE-588)4435196-3 s Chromatographie (DE-588)4010153-8 s DE-604 Schmidt-Traub, Henner 1940-2020 (DE-588)10689496X edt Schulte, Michael (DE-588)1027783600 edt Seidel-Morgenstern, Andreas 1956- (DE-588)114946582 edt Wiley-VCH (DE-588)16179388-5 pbl Erscheint auch als Online-Ausgabe, PDF 978-3-527-81631-6 Erscheint auch als Online-Ausgabe, EPUB 978-3-527-81633-0 Erscheint auch als Online-Ausgabe 978-3-527-81634-7 X:MVB http://www.wiley-vch.de/publish/dt/books/ISBN978-3-527-34486-4/ B:DE-101 application/pdf https://d-nb.info/1198427922/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032227156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Preparative chromatography Chromatographie (DE-588)4010153-8 gnd Präparative Chromatographie (DE-588)4435196-3 gnd |
| subject_GND | (DE-588)4010153-8 (DE-588)4435196-3 (DE-588)4143413-49783030157289 |
| title | Preparative chromatography |
| title_alt | Preparative chromatography of fine chemicals and pharmaceutical agents |
| title_auth | Preparative chromatography |
| title_exact_search | Preparative chromatography |
| title_exact_search_txtP | Preparative chromatography |
| title_full | Preparative chromatography edited by Henner Schmidt-Traub, Michael Schulte, Andreas Seidel-Morgenstern |
| title_fullStr | Preparative chromatography edited by Henner Schmidt-Traub, Michael Schulte, Andreas Seidel-Morgenstern |
| title_full_unstemmed | Preparative chromatography edited by Henner Schmidt-Traub, Michael Schulte, Andreas Seidel-Morgenstern |
| title_short | Preparative chromatography |
| title_sort | preparative chromatography |
| topic | Chromatographie (DE-588)4010153-8 gnd Präparative Chromatographie (DE-588)4435196-3 gnd |
| topic_facet | Chromatographie Präparative Chromatographie Aufsatzsammlung |
| url | http://www.wiley-vch.de/publish/dt/books/ISBN978-3-527-34486-4/ https://d-nb.info/1198427922/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032227156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | UT preparativechromatographyoffinechemicalsandpharmaceuticalagents AT schmidttraubhenner preparativechromatography AT schultemichael preparativechromatography AT seidelmorgensternandreas preparativechromatography AT wileyvch preparativechromatography |
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