Replication independent nucleosome dynamics in Schizosaccharomyces pombe:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Heidelberg
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | 143 Blätter Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Schermann, Géza |d 1984- |e Verfasser |0 (DE-588)1144909538 |4 aut | |
| 245 | 1 | 0 | |a Replication independent nucleosome dynamics in Schizosaccharomyces pombe |c presented by Dr. med. Géza Schermann |
| 264 | 1 | |a Heidelberg |c 2019 | |
| 300 | |a 143 Blätter |b Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Ruperto Carola University Heidelberg |d 2019 | ||
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|---|---|
| adam_text | CONTENTS
1
INTRODUCTION
1
1.1
CHROMATIN
......................................................................................................
1
1.2
CANONICAL
HISTONE
PROTEINS
.........................................................................
3
1.3
HISTONES
AND
NUCLEOSOMES
.........................................................................
4
1.4
POST-TRANSLATIONAL
HISTONE
MODIFICATIONS
...................................................
4
1.5
HISTONE
ACETYLATION
.......................................................................................
6
1.6
HISTONE
METHYLATION
...................................................................................
7
1.7
HISTONE
VARIANTS
.............................................................................................
7
1.8
HISTONE
CHAPERONES
.......................................................................................
8
1.9
CHROMATIN
REMODELLERS
...............................................................................
9
1.10
NUCLEOSOME
DYNAMICS
..................................................................................
11
1.11
PERVASIVE
TRANSCRIPTION
...............................................................................
15
1.12
PERVASIVE
TRANSCRIPTION
AND
CHROMATIN
...................................................
16
1.13
CURRENT
METHODS
.........................................................................................
16
1.14
AIMS
...............................................................................................................
17
2
RESULTS 19
2.1
CHLP-EXO
OPTIMISATION
FOR
S.
POMBE
.........................................................
19
2.2
HISTONE
H3
POSITIONING
AND
OCCUPANCY
...................................................
19
2.3
HISTONE
H2B
POSITIONING
AND
OCCUPANCY
.......................................................
21
2.4
INCREASED
PERVASIVE
TRANSCRIPTION
IN
MUTANT
CELLS
.......................................
22
2.5
H3
TURNOVER
............................................................
23
I
2.6
H2B
TURNOVER
....................................................................................................
28
2.7
HISTONE
ACETYLATION
H3K9/K14
....................................................................
28
3
DISCUSSION
32
3.1
CHLP-EXO
IS
A
VERSATILE
TOOL
IN
S.
POMBE
.......................................................
32
3.2
NUCLEOSOME
POSITIONING
AND
OCCUPANCY
.......................................................
33
3.3
HISTONE
3
TURNOVER
IS
HIGH
AT
THE
PROMOTERS
AND
TERMINATORS,
BUT
LOW
IN
GENE
BODIES
...................................................................................................
35
3.4
HISTONE
3
TURNOVER
CHANGES
IN
CHROMATIN
MUTANTS
....................................
35
3.5
TRANSCRIPTIONALLY
ACTIVE
GENES
SHOW
HIGHER
TURNOVER
.................................
36
3.6
H3K9/K14
ACETYLATION
MARK
IS
ASSOCIATED
WITH
PROMOTERS
.......................
36
3.7
INCREASED
ACETYLATION
CORRELATES
WITH
INCREASED
TURNOVER
..........................
36
3.8
THE
SET
2
MEDIATED
H3K36
METHYLATION
HAS
ONLY
A
MINOR
ROLE
IN
DE
TERMINING
NUCLEOSOME
TURNOVER
..............................................................
37
3.9
CHROMATIN
REMODELLER
MUTANT
STRAINS
SHOWS
VERY
HIGH
TURNOVER
AT
3
END
OF
GENES
......................................................................................................
37
3.10
GENE
CONFIGURATION
DOES
NOT
ALTER
HISTONE
TURNOVER
....................................
38
3.11
CRYPTIC
PROMOTERS
MIGHT
ARISE
FROM
NUCLEOSOME-DEPLETED
REGIONS
...
38
3.12
ANY
NUCLEOSOME-DEPLETED
REGION
CAN
BE
PROMOTERS
....................................
39
3.13
H3K9/K14
ACETYLATION
...................................................................................
40
3.14
HISTONE
2B
TURNOVER
HAPPENS
FAST
AND
AT
AN
EVEN
RATE
OVER
TRANSCRIP
TION
UNITS
................................................................................................
40
3.15
HISTONE
2B
TURNOVER
IS
NOT
AFFECTED
BY
THE
CHROMATIN
MUTANTS
....
41
3.16
MODEL
OF
NUCLEOSOME
TURNOVER
AFFECTING
TRANSCRIPTION
INITIATION
....
41
4
MATERIALS
AND
METHODS
43
4.1
HISTONE
TURNOVER
MEASUREMENTS
...................................................................
43
4.2
CHLP-EXO
.........................................................................................................
46
4.3
STRAINS
USED
IN
THIS
STUDY
.............................................................................
62
4.4
ADAPTERS
USED
IN
CHLP-EXO
.............................................................................
68
II
4.5
PLASMIDS
USED
IN
THIS
STUDY
............................................................................
72
4.6
GROWTH
MEDIA
...............................................................................................
72
4.7
DATABASES
USED
................................................................................................
73
4.8
BIOINFORMATICS
...............................................................................................
74
5
SUPPLEMENTARY
DATA
76
6
WORKFLOWS
AND
SCRIPTS
118
7
LIST
OF
PUBLICATIONS
129
BIBLIOGRAPHY
131
III
|
| adam_txt |
CONTENTS
1
INTRODUCTION
1
1.1
CHROMATIN
.
1
1.2
CANONICAL
HISTONE
PROTEINS
.
3
1.3
HISTONES
AND
NUCLEOSOMES
.
4
1.4
POST-TRANSLATIONAL
HISTONE
MODIFICATIONS
.
4
1.5
HISTONE
ACETYLATION
.
6
1.6
HISTONE
METHYLATION
.
7
1.7
HISTONE
VARIANTS
.
7
1.8
HISTONE
CHAPERONES
.
8
1.9
CHROMATIN
REMODELLERS
.
9
1.10
NUCLEOSOME
DYNAMICS
.
11
1.11
PERVASIVE
TRANSCRIPTION
.
15
1.12
PERVASIVE
TRANSCRIPTION
AND
CHROMATIN
.
16
1.13
CURRENT
METHODS
.
16
1.14
AIMS
.
17
2
RESULTS 19
2.1
CHLP-EXO
OPTIMISATION
FOR
S.
POMBE
.
19
2.2
HISTONE
H3
POSITIONING
AND
OCCUPANCY
.
19
2.3
HISTONE
H2B
POSITIONING
AND
OCCUPANCY
.
21
2.4
INCREASED
PERVASIVE
TRANSCRIPTION
IN
MUTANT
CELLS
.
22
2.5
H3
TURNOVER
.
23
I
2.6
H2B
TURNOVER
.
28
2.7
HISTONE
ACETYLATION
H3K9/K14
.
28
3
DISCUSSION
32
3.1
CHLP-EXO
IS
A
VERSATILE
TOOL
IN
S.
POMBE
.
32
3.2
NUCLEOSOME
POSITIONING
AND
OCCUPANCY
.
33
3.3
HISTONE
3
TURNOVER
IS
HIGH
AT
THE
PROMOTERS
AND
TERMINATORS,
BUT
LOW
IN
GENE
BODIES
.
35
3.4
HISTONE
3
TURNOVER
CHANGES
IN
CHROMATIN
MUTANTS
.
35
3.5
TRANSCRIPTIONALLY
ACTIVE
GENES
SHOW
HIGHER
TURNOVER
.
36
3.6
H3K9/K14
ACETYLATION
MARK
IS
ASSOCIATED
WITH
PROMOTERS
.
36
3.7
INCREASED
ACETYLATION
CORRELATES
WITH
INCREASED
TURNOVER
.
36
3.8
THE
SET
2
MEDIATED
H3K36
METHYLATION
HAS
ONLY
A
MINOR
ROLE
IN
DE
TERMINING
NUCLEOSOME
TURNOVER
.
37
3.9
CHROMATIN
REMODELLER
MUTANT
STRAINS
SHOWS
VERY
HIGH
TURNOVER
AT
3'
END
OF
GENES
.
37
3.10
GENE
CONFIGURATION
DOES
NOT
ALTER
HISTONE
TURNOVER
.
38
3.11
CRYPTIC
PROMOTERS
MIGHT
ARISE
FROM
NUCLEOSOME-DEPLETED
REGIONS
.
38
3.12
ANY
NUCLEOSOME-DEPLETED
REGION
CAN
BE
PROMOTERS
.
39
3.13
H3K9/K14
ACETYLATION
.
40
3.14
HISTONE
2B
TURNOVER
HAPPENS
FAST
AND
AT
AN
EVEN
RATE
OVER
TRANSCRIP
TION
UNITS
.
40
3.15
HISTONE
2B
TURNOVER
IS
NOT
AFFECTED
BY
THE
CHROMATIN
MUTANTS
.
41
3.16
MODEL
OF
NUCLEOSOME
TURNOVER
AFFECTING
TRANSCRIPTION
INITIATION
.
41
4
MATERIALS
AND
METHODS
43
4.1
HISTONE
TURNOVER
MEASUREMENTS
.
43
4.2
CHLP-EXO
.
46
4.3
STRAINS
USED
IN
THIS
STUDY
.
62
4.4
ADAPTERS
USED
IN
CHLP-EXO
.
68
II
4.5
PLASMIDS
USED
IN
THIS
STUDY
.
72
4.6
GROWTH
MEDIA
.
72
4.7
DATABASES
USED
.
73
4.8
BIOINFORMATICS
.
74
5
SUPPLEMENTARY
DATA
76
6
WORKFLOWS
AND
SCRIPTS
118
7
LIST
OF
PUBLICATIONS
129
BIBLIOGRAPHY
131
III |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Schermann, Géza 1984- |
| author_GND | (DE-588)1144909538 |
| author_facet | Schermann, Géza 1984- |
| author_role | aut |
| author_sort | Schermann, Géza 1984- |
| author_variant | g s gs |
| building | Verbundindex |
| bvnumber | BV046625886 |
| ctrlnum | (OCoLC)1145265376 (DE-599)DNB1204379572 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Thesis Book |
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| id | DE-604.BV046625886 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T14:09:20Z |
| indexdate | 2024-07-10T08:49:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032037527 |
| oclc_num | 1145265376 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 143 Blätter Diagramme 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Schermann, Géza 1984- Verfasser (DE-588)1144909538 aut Replication independent nucleosome dynamics in Schizosaccharomyces pombe presented by Dr. med. Géza Schermann Heidelberg 2019 143 Blätter Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruperto Carola University Heidelberg 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1204379572/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032037527&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schermann, Géza 1984- Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| subject_GND | (DE-588)4113937-9 |
| title | Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| title_auth | Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| title_exact_search | Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| title_exact_search_txtP | Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| title_full | Replication independent nucleosome dynamics in Schizosaccharomyces pombe presented by Dr. med. Géza Schermann |
| title_fullStr | Replication independent nucleosome dynamics in Schizosaccharomyces pombe presented by Dr. med. Géza Schermann |
| title_full_unstemmed | Replication independent nucleosome dynamics in Schizosaccharomyces pombe presented by Dr. med. Géza Schermann |
| title_short | Replication independent nucleosome dynamics in Schizosaccharomyces pombe |
| title_sort | replication independent nucleosome dynamics in schizosaccharomyces pombe |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1204379572/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032037527&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT schermanngeza replicationindependentnucleosomedynamicsinschizosaccharomycespombe |
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