Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Bonn
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 74 Seiten Illustrationen 21 cm |
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| 245 | 1 | 0 | |a Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |c Qian Li |
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Record in the Search Index
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| adam_text | 3
CONTENTS
LIST
OF
ABBREVIATIONS
...............................................................................................................
5
1.
INTRODUCTION
........................................................................................................................
6
1.1
CORONARY
ARTERY
DISEASE
(CAD)
AND
CURRENT
BIOMARKERS
.........................................
6
1.2
LONG
NONCODING
RNA
AND
CAD
.................................................................................
7
1.3
EXTRACELLULAR
VESICLES
AND
CAD
..................................................................................
8
1.4
AIMS
............................................................................................................................
10
2.
MATERIAL
AND
METHODS
......................................................................................................
11
2.1
CLINICAL
STUDY
DESIGN
.................................................................................................
11
2.2
STUDY
SUBJECTS
............................................................................................................
12
2.3
PREPARATION
OF
BLOOD
SAMPLES
...................................................................................
12
2.4
SEV
ISOLATION
FROM
PLASMA
.........................................................................................
13
2.5
CELL
CULTURE,
ENDOTHELIAL
SEV
GENERATION
..................................................................
15
2.6
NANOPARTICLE
TRACKING
ANALYSIS
..................................................................................
15
2.7
EXOCET
EXOSOME
QUANTITATION
ASSAY
.....................................................................
16
2.8
RNA
ISOLATION
AND
REAL-TIME
PCR
.............................................................................
16
2.9
PUNISHER
EXPRESSION
CALCULATION
IN
TARGET
HCAEC
USING
COPY
NUMBER
.............
16
2.10
WESTERN
BLOT
............................................................................................................
17
2.11
RNA
DEGRADATION
ASSAY
..........................................................................................
17
2.12
STABILITY
OF
PUNISHER
IN
SEV
FROM
PLASMA
UNDER
DIFFERENT
STORAGE
CONDITIONS..
18
2.13
EXPRESSION
OF
PUNISHER
UNDER
ATHEROSCLEROTIC
CONDITIONS
TREATED
EC
AND
SEV
18
2.14
ENDOTHELIAL
SEV
WERE
ABSORBED
INTO
RECIPIENT
EC
.................................................
18
2.15
KNOCKDOWN
EXPERIMENTS
ON
EC
.............................................................................
19
2.16
MODIFIED
SEV
GENERATION
.........................................................................................
19
2.17
SCRATCH
ASSAY
..........................................................................................................
19
2.18
PROLIFERATION
ASSAY
BY
FLUORESCENCE
MICROSCOPY
...................................................
19
2.19
MATRIGEL-BASED
TUBE
FORMATION
ASSAY
.....................................................................
20
2.20
BOYDEN
CHAMBER
ASSAY
..........................................................................................
20
2.21
STATISTICAL
ANALYSIS
...................................................................................................
20
4
3.
RESULTS
..............................................................................................................................22
3.1
PLASMA
SEV-ENRICHED
PUNISHER
ACTS
AS
POTENTIAL
DIAGNOSTIC
BIOMARKER
FOR
CAD
22
3.1.1
IDENTIFICATION
OF
CANDIDATE
INCRNAS
................................................................... 22
3.1.2
THE
VALIDATION
PHASE
AND
BIOMARKER
EVALUATION
............................................... 27
3.1.3
SEV
ISOLATED
FROM
PLASMA
ARE
MAINLY
EXOSOME-LIKE
VESICLES
........................... 35
3.2
ATHEROSCLEROTIC
STIMULI
INCREASE
SEM-PUNISHER
EXPRESSION
IN
VITRO
.....................
41
3.3
PUNISHER
IN
ENDOTHELIAL-SEV
REGULATED
TARGET
EC
FUNCTION
.................................
43
4.
DISCUSSION
........................................................................................................................53
5.
CONCLUSION
........................................................................................................................57
6.
SUMMARY
..........................................................................................................................58
7.
LIST
OF
FIGURES
...................................................................................................................
59
8.
LIST
OF
TABLES
....................................................................................................................60
9.
REFERENCES
.......................................................................................................................
61
10.
ACKNOWLEDGMENTS
.........................................................................................................
74
|
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| author_facet | Li, Qian |
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| building | Verbundindex |
| bvnumber | BV046355216 |
| ctrlnum | (OCoLC)1137826806 (DE-599)DNB1197167269 |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T08:42:32Z |
| institution | BVB |
| language | English |
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| physical | 74 Seiten Illustrationen 21 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
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| spelling | Li, Qian Verfasser (DE-588)1196380945 aut Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease Qian Li Bonn 2019 74 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinische Friedrich-Wilhelms-Universität Bonn 2019 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031731618&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Li, Qian Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |
| subject_GND | (DE-588)4113937-9 |
| title | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |
| title_auth | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |
| title_exact_search | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |
| title_full | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease Qian Li |
| title_fullStr | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease Qian Li |
| title_full_unstemmed | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease Qian Li |
| title_short | Small extracellular vesicle-associated long noncoding RNA punisher as a novel plasma-based biomarker in coronary artery disease |
| title_sort | small extracellular vesicle associated long noncoding rna punisher as a novel plasma based biomarker in coronary artery disease |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031731618&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT liqian smallextracellularvesicleassociatedlongnoncodingrnapunisherasanovelplasmabasedbiomarkerincoronaryarterydisease |