Microfluidic biochip for studying electrotaxis of cells under direct current electric fields:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Dresden
TUDpress
2019
|
| Series: | Dresdner Beiträge zur Sensorik
73 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XII, 126 Seiten Illustrationen |
| ISBN: | 9783959081672 3959081677 |
Staff View
MARC
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| 100 | 1 | |a de Sousa Gonçalves Rio, Marisa |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Microfluidic biochip for studying electrotaxis of cells under direct current electric fields |c Marisa de Sousa Gonçalves Rio |
| 263 | |a 201906 | ||
| 264 | 1 | |a Dresden |b TUDpress |c 2019 | |
| 300 | |a XII, 126 Seiten |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Dresdner Beiträge zur Sensorik |v 73 | |
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| 650 | 0 | 7 | |a Biochip |0 (DE-588)4270225-2 |2 gnd |9 rswk-swf |
| 653 | |a Paperback / softback | ||
| 653 | |a biochip | ||
| 653 | |a electric fields | ||
| 653 | |a microfluidic | ||
| 653 | |a 1684: Hardcover, Softcover / Technik/Elektronik, Elektrotechnik, Nachrichtentechnik | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031697213 | ||
Record in the Search Index
| _version_ | 1804180801866170368 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ABSTRACT
IV
ZUSAMMENFASSUNG
V
LIST
OF
ABBREVIATIONS
X
LIST
OF
SYMBOLS
XII
1
INTRODUCTION
1
1.1
MOTIVATION
....................................................................................................
1
1.2
STATE
OF
THE
ART
...........................................................................................
2
1.3
SCOPE
AND
REQUIREMENTS
............................................................................
9
1.4
OVERVIEW
OF
THE
DISSERTATION
......................................................................
10
2
PROCESSES
AND
FUNDAMENTALS
13
2.1
BIOLOGICAL
BACKGROUND
...............................................................................
14
2.1.1
BIOELECTRICITY
..................................................................................
14
2.1.2
DIRECT
CURRENT
VOLTAGE
SOURCES
IN
BIOLOGICAL
ORGANISMS
.................
16
2.1.3
EFFECTS
OF
APPLIED
DC
EFS
AT
CELLULAR
LEVEL
...................................
22
2.2
ELECTROCHEMISTRY
........................................................................................
25
2.2.1
TRANSITION
FROM
ELECTRONIC
TO
IONIC
CONDUCTION
...........................
25
2.2.2
DIFFUSION,
MIGRATION
AND
CONVECTION
IN
MICROFLUIDIC
SYSTEMS
...
27
2.2.3
ELECTROLYSIS
IN
AQUEOUS
NACL
SOLUTIONS
........................................
29
2.3
APPLICATION
OF
STATIONARY
ELECTRIC
FIELDS
.....................................................
31
2.4
JOULE
HEATING
INDUCED
BY
ELECTRIC
FIELD
APPLICATION
...................................
32
3
MATERIALS
AND
METHODS
33
3.1
PHOTORESIST
SU-8
........................................................................................
34
3.2
POLY
(
DIMETHY
LSYLOXANE)
...............................................................................
35
3.3
IMAGING
TECHNIQUES
.....................................................................................
36
3.3.1
OPTICAL
MICROSCOPY
.........................................................................
36
3.3.2
FLUORESCENCE
MICROSCOPY
................................................................
37
3.3.3
PHASE
CONTRAST
MICROSCOPY
..........................................................
39
3.4
CELL
CULTURE
AND
PREPARATION
......................................................................
39
3.5
CELL
STAINING
..................................................................................................
40
3.5.1
LIFE-DEAD
ASSAY
...............................................................................
40
3.5.2
IMMUNOFLUORESCENCE
STAINING
..........................................................
41
3.6
CELL
TRACKING
AND
EVALUATION
OF
CELL
MIGRATION
...........................................
42
3.7
WOUND
HEALING
ASSAY
..................................................................................
42
3.8
MEASUREMENT
OF
PH
WITH
FLUORESCENT
DYES
.................................................
43
4
FABRICATION
PRINCIPLES
AND
DEVICE
ASSEMBLY
45
4.1
MICROFLUIDIC
SYSTEM
.....................................................................................
46
4.1.1
SILICON
MASTER
..................................................................................
46
4.1.2
SOFT
LITHOGRAPHY
...............................................................................
47
4.1.3
MICCELL
MOLDING
STATION
................................................................
48
4.2
SU-8
FREE-STANDING
MEMBRANE
...................................................................
50
4.3
SURFACE
MODIFICATION
..................................................................................
51
4.3.1
SILANIZATION
BY
APTES-TREATMENT
.................................................
52
4.3.2
SILANIZATION
BY
VAPOR-PHASE
DEPOSITION
........................................
54
4.3.3
ALTERNATIVE
METHODS
......................................................................
55
4.4
FABRICATION
OF
THE
AG/AGCL
ELECTRODES
....................................................
55
4.5
DEVICE
ASSEMBLY
...........................................................................................
59
4.5.1
SET-UP
AND
ASSEMBLY
CONCEPT
..........................................................
59
4.5.2
ALIGNMENT
OF
BIOCHIP
COMPONENTS
.................................................
60
4.5.3
ELECTROLYTE
INTO
THE
MICROFLUIDIC
CHANNELS
.....................................
61
4.6
CONCLUSIONS
.................................................................................................
62
5
CHARACTERIZATION
OF
THE
FUNCTIONALIZED
SURFACES
65
5.1
CONTACT
ANGLE
MEASUREMENTS
......................................................................
66
5.1.1
BASICS
..............................................................................................
66
5.1.2
EXPERIMENTAL
PROCEDURE
................................................................
68
5.1.3
RESULTS
AND
DISCUSSION
...................................................................
69
5.2
X-RAY
PHOTOELECTRON
SPECTROSCOPY
.............................................................
70
5.2.1
EXPERIMENTAL
PROCEDURE
................................................................
71
5.2.2
RESULTS
AND
DISCUSSION
...................................................................
71
VII
5.3
ATOMIC
FORCE
MICROSCOPY
............................................................................
72
5.3.1
BASICS
..............................................................................................
72
5.3.2
EXPERIMENTAL
PROCEDURE
................................................................
73
5.3.3
RESULTS
AND
DISCUSSION
...................................................................
74
5.4
CELL
BIOCOMPATIBILITY
..................................................................................
76
5.4.1
BASICS
..............................................................................................
76
5.4.2
EXPERIMENTAL
PROCEDURE
................................................................
76
5.4.3
RESULTS
AND
DISCUSSION
...................................................................
77
5.5
CONCLUSIONS
.................................................................................................
78
6
DEVICE
CHARACTERIZATION
81
6.1
JOULE
HEATING
DUE
TO
APPLIED
ELECTRIC
FIELD
................................................
82
6.2
NUMERICAL
SIMULATION
OF
ELECTRIC
FIELD
STRENGTH
DISTRIBUTIONS
..................
84
6.2.1
MODELING
THE
MICROFLUIDIC
SYSTEM
................................................
84
6.2.2
ELECTRIC
FIELD
SIMULATION
................................................................
84
6.3
PH
ESTIMATION
FOLLOWING
A
LONG
TERM
EXPERIMENT
....................................
87
6.4
SUITABILITY
FOR
MULTIPLE
USE
.........................................................................
89
6.5
CONCLUSIONS
.................................................................................................
91
7
CELL
EXPERIMENTS
93
7.1
ELECTROTAXIS
EXPERIMENTS
............................................................................
94
7.2
CELL
VIABILITY
.................................................................................................
95
7.3
IMMUNOFLUORESCENCE
.....................................................................................
96
7.4
WOUND
HEALING
ASSAY
..................................................................................
98
7.5
CONCLUSIONS
....................................................................................................
100
8
CONCLUSIONS
AND
OUTLOOK
103
8.1
CONCLUSIONS
....................................................................................................
103
8.2
OUTLOOK
..........................................................................................................
105
APPENDIX
A
CURRICULUM
VITAE
107
APPENDIX
B
LIST
OF
RELEVANT
PUBLICATIONS
109
B.L
JOURNAL
ARTICLES
.............................................................................................
109
B.2
CONFERENCE
CONTRIBUTIONS
..............................................................................
109
BIBLIOGRAPHY
111
VIII
|
| any_adam_object | 1 |
| author | de Sousa Gonçalves Rio, Marisa |
| author_facet | de Sousa Gonçalves Rio, Marisa |
| author_role | aut |
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| author_variant | s g r m d sgrm sgrmd |
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| discipline | Elektrotechnik / Elektronik / Nachrichtentechnik Mess-/Steuerungs-/Regelungs-/Automatisierungstechnik / Mechatronik |
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| id | DE-604.BV046320180 |
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| indexdate | 2024-07-10T08:41:32Z |
| institution | BVB |
| isbn | 9783959081672 3959081677 |
| language | English |
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| physical | XII, 126 Seiten Illustrationen |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| publisher | TUDpress |
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| series | Dresdner Beiträge zur Sensorik |
| series2 | Dresdner Beiträge zur Sensorik |
| spelling | de Sousa Gonçalves Rio, Marisa Verfasser aut Microfluidic biochip for studying electrotaxis of cells under direct current electric fields Marisa de Sousa Gonçalves Rio 201906 Dresden TUDpress 2019 XII, 126 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Dresdner Beiträge zur Sensorik 73 Galvanotaxis (DE-588)4155914-9 gnd rswk-swf Mikrofluidik (DE-588)4803438-1 gnd rswk-swf Biochip (DE-588)4270225-2 gnd rswk-swf Paperback / softback biochip electric fields microfluidic 1684: Hardcover, Softcover / Technik/Elektronik, Elektrotechnik, Nachrichtentechnik (DE-588)4113937-9 Hochschulschrift gnd-content Galvanotaxis (DE-588)4155914-9 s Biochip (DE-588)4270225-2 s Mikrofluidik (DE-588)4803438-1 s DE-604 Dresdner Beiträge zur Sensorik 73 (DE-604)BV011109099 73 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031697213&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | de Sousa Gonçalves Rio, Marisa Microfluidic biochip for studying electrotaxis of cells under direct current electric fields Dresdner Beiträge zur Sensorik Galvanotaxis (DE-588)4155914-9 gnd Mikrofluidik (DE-588)4803438-1 gnd Biochip (DE-588)4270225-2 gnd |
| subject_GND | (DE-588)4155914-9 (DE-588)4803438-1 (DE-588)4270225-2 (DE-588)4113937-9 |
| title | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields |
| title_auth | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields |
| title_exact_search | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields |
| title_full | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields Marisa de Sousa Gonçalves Rio |
| title_fullStr | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields Marisa de Sousa Gonçalves Rio |
| title_full_unstemmed | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields Marisa de Sousa Gonçalves Rio |
| title_short | Microfluidic biochip for studying electrotaxis of cells under direct current electric fields |
| title_sort | microfluidic biochip for studying electrotaxis of cells under direct current electric fields |
| topic | Galvanotaxis (DE-588)4155914-9 gnd Mikrofluidik (DE-588)4803438-1 gnd Biochip (DE-588)4270225-2 gnd |
| topic_facet | Galvanotaxis Mikrofluidik Biochip Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031697213&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011109099 |
| work_keys_str_mv | AT desousagoncalvesriomarisa microfluidicbiochipforstudyingelectrotaxisofcellsunderdirectcurrentelectricfields |