Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Ulm
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | IX, 70, XXIII Blätter Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Braun, Elisabeth |e Verfasser |0 (DE-588)1189952882 |4 aut | |
| 245 | 1 | 0 | |a Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |c Elisabeth Braun |
| 264 | 1 | |a Ulm |c 2019 | |
| 300 | |a IX, 70, XXIII Blätter |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Ulm |d 2019 | ||
| 653 | |a Acquired immunodeficiency syndrome | ||
| 653 | |a Glycoproteins | ||
| 653 | |a Immunology | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a Universität Ulm |0 (DE-588)30607-1 |4 dgg | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031649249 | ||
Record in the Search Index
| _version_ | 1804180715153129472 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.........................................................................................................................
ILL
ABBREVIATIONS
...............................................................................................................................
V
1
INTRODUCTION
............................................................................................................................
1
1.1
THE
HUMAN
IMMUNODEFICIENCY
VIRUS
AND
ITS
REPLICATION
CYCLE
.................................
1
1.2
THE
ENVELOPE
GLYCOPROTEIN
OF
HIV
..............................................................................
3
1.3
RESTRICTION
FACTORS
.........................................................................................................
4
1.4
GUANYLATE-BINDING
PROTEINS
..........................................................................................
6
1.5
THE
PROPROTEIN
CONVERTASE
FURIN
...................................................................................
9
2
SCIENTIFIC
AIMS
....................................................................................................................
12
3
MATERIAL
AND
METHODS
......................................................
13
3.1
MATERIAL
.........................................................................................................................
13
3.1.1
CELL
CULTURE
.........................................................................................................
13
3.1.2
MEDIA
..................................................................................................................
14
3.1.3
NUCLEIC
ACIDS
......................................................................................................
15
3.1.4
VIRUS
STRAINS
......................................................................................................
20
3.1.5
KITS
AND
REAGENTS
...............................................................................................
20
3.1.6
ENZYMES
.............................................................................................................
22
3.1.7
SOLUTIONS
AND
BUFFERS
.......................................................................................
23
3.1.8
ANTIBODIES
..........................................................................................................
24
3.1.9
CONSUMABLES
.....................................................................................................
26
3.1.10
EQUIPMENT
..........................................................................................................
26
3.1.11
SOFTWARE
.............................................................................................................
27
3.2
METHODS
.........................................................................................................................
28
3.2.1
CLONING
...............................................................................................................
28
3.2.2
CELL
CULTURE
........................................................................................................
29
3.2.3
TRANSFECTION
......................................................................................................
30
3.2.4
GENERATION
AND
TITER
DETERMINATION
OF
MEASLES
AND
INFLUENZA
VIRUS
STOCKS3
1
3.2.5
STIMULATION
OF
MDMS
AND
CD4
+
T
CELLS
.......................................................
32
3.2.6
INFECTION
OF
HLACS
.........................................................................................
32
3.2.7
SIRNA
TRANSFECTION
AND
INFECTION
OF
MDMS
.................................................
33
3.2.8
INFECTION
OF
THP-1
CELLS
WITH
MEASLES,
INFLUENZA
AND
ZIKA
VIRUS
..............
33
3.2.9
VIRUS
RELEASE
ASSAYS
..........................................................................................
35
3.2.10
FLOW
CYTOMETRY
.................................................................................................
36
3.2.11
WESTERN
BLOT
......................................................................................................37
3.2.12
CO-IMMUNOPRECIPITATION
..................................................................................
39
3.2.13
MICROSCOPY
........................................................................................................
40
3.2.14
4G2
IMMUNOSTAINING
........................................................................................
40
3.2.15
FURIN
ACTIVITY
ASSAY
...........................................................................................
41
3.2.16
FIREFLY
LUCIFERASE
ASSAY
....................................................................................
41
3.2.17
STATISTICAL
ANALYSIS
.............................................................................................
42
3.2.18
ETHICAL
STATEMENT
...............................................................................................
42
4
RESULTS
.................................................................................................................................
43
4.1
GBP2
AND
GBP5
INTERFERE
WITH
HIV-1
ENV
MATURATION
AND
REDUCE
INFECTIOUS
VIRUS
YIELD
IN
AN
ISOPRENYLATION-DEPENDENT
MANNER
........................................................ 43
4.2
GBP2
AND
GBP5
ARE
INTERFERON-INDUCIBLE
PROTEINS
THAT
RESTRICT
HIV-1
IN
PRIMARY
TARGET
CELLS
..................................................................................................................
45
4.3
GBP2
AND
GBPS
DO
NOT
DIRECTLY
INTERACT
WITH
ENV
TO
RESTRICT
HIV-1
..................50
4.4
GBP2
AND
GBPS
RESTRICT
HIV-1
BY
TARGETING
THE
CELLULAR
PROTEASE
FURIN
............ 52
4.5
GBP2
AND
GBPS
INHIBIT
MATURATION
OF
DIVERSE
VIRAL
GLYCOPROTEINS
AND
RESTRICT
ZIKA,
INFLUENZA
A
AND
MEASLES
VIRUSES
.................................................................... 57
5
DISCUSSION
...........................................................................................................................
61
6
SUMMARY
.............................................................................................................................69
7
REFERENCES
............................................................................................................................
X
8
ACKNOWLEDGEMENTS
.......................................................................................................
XXII
9
STATUTORY
DECLARATION
...................................................................................................
XXIII
|
| any_adam_object | 1 |
| author | Braun, Elisabeth |
| author_GND | (DE-588)1189952882 |
| author_facet | Braun, Elisabeth |
| author_role | aut |
| author_sort | Braun, Elisabeth |
| author_variant | e b eb |
| building | Verbundindex |
| bvnumber | BV046271448 |
| ctrlnum | (OCoLC)1129399323 (DE-599)KXP1677345012 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV046271448 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:40:10Z |
| institution | BVB |
| institution_GND | (DE-588)30607-1 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031649249 |
| oclc_num | 1129399323 |
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| owner | DE-91 DE-BY-TUM DE-355 DE-BY-UBR |
| owner_facet | DE-91 DE-BY-TUM DE-355 DE-BY-UBR |
| physical | IX, 70, XXIII Blätter Illustrationen, Diagramme 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Braun, Elisabeth Verfasser (DE-588)1189952882 aut Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin Elisabeth Braun Ulm 2019 IX, 70, XXIII Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2019 Acquired immunodeficiency syndrome Glycoproteins Immunology (DE-588)4113937-9 Hochschulschrift gnd-content Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf https://d-nb.info/1196982740/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031649249&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Braun, Elisabeth Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| subject_GND | (DE-588)4113937-9 |
| title | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| title_auth | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| title_exact_search | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| title_full | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin Elisabeth Braun |
| title_fullStr | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin Elisabeth Braun |
| title_full_unstemmed | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin Elisabeth Braun |
| title_short | Guanylate-binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| title_sort | guanylate binding proteins 2 and 5 are broadly active restriction factors that inhibit viral envelope glycoprotein processing by targeting the cellular protease furin |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1196982740/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031649249&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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