Macrophage function in corneal hem- and lymphangiogensis:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Köln
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | 97 Seiten Illustrationen, Diagramme 21 cm |
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MARC
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| 245 | 1 | 0 | |a Macrophage function in corneal hem- and lymphangiogensis |c vorgelegt von Anne Kiesewetter |
| 264 | 1 | |a Köln |c 2019 | |
| 300 | |a 97 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Universität zu Köln |d 2019 | ||
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|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
1
ABSTRACT
....................................................................................................................................
1
1.1
ZUSAMMENFASSUNG
.............................................................................................................
1
1.2
ABSTRACT
.............................................................................................................................
4
2
INTRODUCTION
............................................................................................................................
6
2.1
OVERVIEW
OF
CORNEAL
ANATOMY
...........................................................................................
6
2.2
THE
CORNEAL
ANGIOGENIC
AND
IMMUNE
PRIVILEGE
................................................................
7
2.3
CORNEAL
NEOVASCULARIZATION
..............................................................................................
9
2.4
MACROPHAGES
IN
CORNEAL
ANGIOGENESIS
AND WOUND
HEALING
............................................
10
2.5
MECHANISMS
OF
MACROPHAGE
RECRUITMENT
IN
THE
CORNEA
.................................................
12
2.5.1
THE
CHEMOKINE
RECEPTOR
CCR2
AND
ITS
LIGAND
CCL2
.......................................................
14
2.5.2
THE
CHEMOKINE
RECEPTOR
CX3CR1
AND
ITS
LIGAND
CX3CL1
...............................................
15
2.6
CORNEAL
INJURY
MODELS
IN
MICE
........................................................................................
16
2.7
AIMS
OF
THE
THESIS
............................................................................................................
18
3
MATERIALS
AND
METHODS
........................................................................................................
20
3.1
MATERIALS
.........................................................................................................................
20
3.1.1
EQUIPMENT
...............................................................................................................
20
3.1.2
KITS
..........................................................................................................................
20
3.1.3
CHEMICALS
AND
REAGENTS
..........................................................................................
21
3.1.4
CONSUMABLES
...........................................................................................................
21
3.1.5
SUPPLEMENTS,
MEDIA
AND
BUFFERS
FOR
CELL
CULTURE
....................................................
22
3.1.6
MEDIA
FOR
CELL
CULTURE
..............................................................................................
22
3.1.7
CYTOKINES
FOR
CELL
CULTURE
........................................................................................
22
3.1.8
COMPOUNDS
FOR
INJECTION
(ANIMAL
EXPERIMENTS)
.....................................................
22
3.1.9
COMPOUNDS
FOR
TOPICAL
TREATMENT
(ANIMAL
EXPERIMENTS)
.......................................
22
3.1.10
SOFTWARES
.................................................................................................................
23
3.1.11
ANTIBODIES
.......................................................
23
3.1.12
PRIMERS
FOR
PCR
......................................................................................................
24
3.1.13
MOUSE
STRAINS
..........................................................................................................
25
3.2
METHODS
..........................................................................................................................
26
3.2.1
MICE
AND
ANESTHESIA
...............................................................................................
26
3.2.2
CORNEAL
INCISION
MODEL
...........................................................................................
26
3.2.3
SUTURE-INDUCED
CORNEAL
NEOVASCULARIZATION
...........................................................
27
3.2.4
SUBCONJUNCTIVAL
INJECTIONS
......................................................................................
27
3.2.5
SYSTEMIC
INJECTION
OF
CLODRONATE
.............................................................................
27
II
TABLE
OF
CONTENTS
3.2.6
PHASE-SPECIFIC
MACROPHAGE
DEPLETION
.....................................................................
27
3.2.7
APPLICATION
OF
VEGFR3
BLOCKING
ANTIBODY
...............................................................
27
3.2.8
APPLICATION
OF
DIPHTHERIA
TOXIN
IN
IDTRLYSMCRE
MICE
...........................................
28
3.2.9
ISOLATION
OF
BONE
MARROW-DERIVED
MACROPHAGES
...................................................
28
3.2.10
MACROPHAGE
CULTURE
AND
STIMULATION
............................
28
3.2.11
RNA
ISOLATION
FROM
MACROPHAGES
............................................................................
29
3.2.12
RNA
ISOLATION
FROM
CORNEAL
TISSUE
...........................................................................
29
3.2.13
CDNA
SYNTHESIS
........................................................................................................
29
3.2.14
REAL-TIME
PCR
..........................................................................................................
29
3.2.15
PCR
FOR
GENOTYPING
.................................................................................................
30
3.2.16
DNA
AGAROSE
GEL
ELECTROPHORESIS
.............................................................................
30
3.2.17
STAINING
OF
CORNEAL
WHOLE
MOUNTS
...........................................................................
31
3.2.18
REMOVAL
OF
CORNEAL
EPITHELIUM
................................................................................
31
3.2.19
CORNEAL
WHOLE
MOUNT
ANALYSIS
................................................................................
31
3.2.20
MEASUREMENT
OF
VESSEL
INVASION
DISTANCE
...............................................................
32
3.2.21
PREPARATION
AND
IMMUNOSTAINING
OF
CORNEAL
CRYOSECTIONS
.....................................
33
3.2.22
BRDU
PULSE
LABELLING
AND
ANTI-BRDU
STAINING
OF
CRYOSECTIONS
.................................
35
3.2.23
STATISTICAL
ANALYSES
..................................................................................................
35
4
RESULTS
.....................................
36
4.1
CORNEAL
MACROPHAGE
INFILTRATION AND
ANGIOGENESIS
FOLLOW
DIFFERENT
DYNAMICS
DEPENDING
ON
THE
TYPE
OF
INJURY
..................................................................................................................
36
4.1.1
MACROPHAGE
INFILTRATION
AND
LYMPHANGIOGENESIS
ARE
TRANSIENT
IN
THE
INCISION
MODEL
OF
ACUTE
CORNEAL
INJURY
...................................................................................................................
36
4.1.2
MACROPHAGE
INFILTRATION
AND
NEOVESSELS
ARE
PERSISTENT
IN
THE
SUTURE
PLACEMENT
MODEL
OF
CHRONIC
CORNEAL
INJURY
.................................................................................................................
38
4.2
ESTABLISHMENT
OF
CORNEAL
MACROPHAGE
DEPLETION
...........................................................
40
4.3
MAINTENANCE
OF
THE
RESTING
LIMBAL
BLOOD
AND
LYMPHATIC
VASCULATURE
IS
INDEPENDENT
OF
MACROPHAGES
...............................................................................................................................
43
4.4
MACROPHAGES
MEDIATE
DISTINCT
VASCULAR
RESPONSES
DEPENDING
ON
THE
TYPE
OF
CORNEAL
INJURY
44
4.4.1
MACROPHAGES
MEDIATE
LV
SPROUTING
AFTER
INCISION
INJURY
...............................................
46
4.4.2
MACROPHAGES
INITIATE
BV
AND
LV
SPROUTING
AND ADDITIONALLY
MEDIATE
LV
MAINTENANCE
AFTER
SUTURE
PLACEMENT
................................................................................................................
47
4.5
MACROPHAGE-MEDIATED
LV
MAINTENANCE
IN
THE
SUTURE
MODEL
IS
INDEPENDENT
OF
VEGFR-3
SIGNALING
.....................................................................................................................................48
4.6
THE
NATURE
OF
THE
CORNEAL
INJURY
RESPONSE
DETERMINES
MACROPHAGE
ACTIVATION
AND
EXPRESSION
OF
PROANGIOGENIC
FACTORS
.........................................................................................
53
4.7
ROLE
OF
CCR2
AND
CX3CR1
IN
MACROPHAGE
RECRUITMENT
AND
CORNEAL
ANGIOGENESIS
.......
56
TABLE
OF
CONTENTS
III
4.7.1.
CCR2
AND
CX3CR1
SHOW
DIFFERENT
EXPRESSION
PROFILES
IN
THE
INJURED
CORNEA
........
57
4.7.2.
CCR2
AND
CX3CR1
APPEAR
TO
BE
DISPENSABLE
FOR
MACROPHAGE
RECRUITMENT
AND
CORNEAL
ANGIOGENESIS
..............................................................................................................
60
4.8
MACROPHAGES
PROLIFERATE
IN
CHRONIC,
BUT
NOT
ACUTE
CORNEAL
INJURY
......................................
68
5
DISCUSSION
.............................................................................................................................
72
5.1
THE
NATURE
OF
CORNEAL
INJURY
DETERMINES
THE
TYPE
OF
THE
VASCULAR
RESPONSE
VIA
MACROPHAGE
ACTIVATION
...............................................................................................................
72
5.2
MACROPHAGES
DISPLAY
DISTINCT
PHASE-SPECIFIC
FUNCTIONS
DURING
CORNEAL
ANGIOGENESIS....
73
5.3
CCR2
AND
CX3CR1
APPEAR
TO BE
DISPENSABLE
FOR
MACROPHAGE
RECRUITMENT
AND
ANGIOGENESIS
...............................................................................................................................
76
5.4
CONCLUSIONS
AND
OUTLOOK
................................................................................................
80
6
REFERENCES
.................
83
7
SUPPLEMENT
...........................................................................................................................
95
7.1
ABBREVIATIONS
.................................................................................................................
95
7.2
DANKSAGUNG
.....................................................................................................................
96
7.3
ERKLARUNG
........................................................................................................................97
|
| any_adam_object | 1 |
| author | Kiesewetter, Anne |
| author_GND | (DE-588)1191273105 |
| author_facet | Kiesewetter, Anne |
| author_role | aut |
| author_sort | Kiesewetter, Anne |
| author_variant | a k ak |
| building | Verbundindex |
| bvnumber | BV046260830 |
| ctrlnum | (OCoLC)1128852562 (DE-599)DNB1192917685 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T08:39:51Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031638835 |
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| open_access_boolean | |
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| owner_facet | DE-355 DE-BY-UBR |
| physical | 97 Seiten Illustrationen, Diagramme 21 cm |
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| publishDateSearch | 2019 |
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| spelling | Kiesewetter, Anne Verfasser (DE-588)1191273105 aut Macrophage function in corneal hem- and lymphangiogensis vorgelegt von Anne Kiesewetter Köln 2019 97 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1192917685/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031638835&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kiesewetter, Anne Macrophage function in corneal hem- and lymphangiogensis |
| subject_GND | (DE-588)4113937-9 |
| title | Macrophage function in corneal hem- and lymphangiogensis |
| title_auth | Macrophage function in corneal hem- and lymphangiogensis |
| title_exact_search | Macrophage function in corneal hem- and lymphangiogensis |
| title_full | Macrophage function in corneal hem- and lymphangiogensis vorgelegt von Anne Kiesewetter |
| title_fullStr | Macrophage function in corneal hem- and lymphangiogensis vorgelegt von Anne Kiesewetter |
| title_full_unstemmed | Macrophage function in corneal hem- and lymphangiogensis vorgelegt von Anne Kiesewetter |
| title_short | Macrophage function in corneal hem- and lymphangiogensis |
| title_sort | macrophage function in corneal hem and lymphangiogensis |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1192917685/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031638835&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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