Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Erlangen ; Nürnberg
[2019]
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | VI, 91 Seiten Illustrationen, Diagramme 21 cm |
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| 245 | 1 | 0 | |a Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis |c vorgelegt von Benjamin Martin Häberle |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2019] | |
| 300 | |a VI, 91 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
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Record in the Search Index
| _version_ | 1804180631083548672 |
|---|---|
| adam_text | C
ONTENTS
LIST
OF
FIGURES
..............................................................................................................................
IV
ABBREVIATIONS
................................................................................................................................
V
INTRODUCTION
..................................................................................................................................
1
HIPPOCAMPAL
DENTATE
GYRUS
AND
MEMORY
..................................................................
1
ADULT
NEUROGENESIS
AND
DENTATE
GYRUS
FUNCTION
...............................................
2
GENERATION
OF
NEW
NEURONS
FROM
STEM
CELLS
........................................................ 4
REGULATION
OF
CELLULAR
DEVELOPMENT
FROM
STEM
CELLS
TO
MATURE
GRANULE
CELLS
DURING
AHN
..................................................................................................
7
SOXC
-
MULTIFUNCTIONAL
REGULATORS
OF
NEURODEVELOPMENT
..........................
9
GOAL
AND
AIM
OF
THE
THESIS
...................................................................................................
12
RESULTS
.............................................................................................................................................
13
SOX11
IS
EXPRESSED
IN
DOX
EXPRESSING
NEUROBLASTS
AND
IMMATURE
NEURONS
.......................................................................................................................................
13
OCX-DRIVEN
CREER
12
ACTIVITY
ABLATES
SOX11
EXPRESSION
IN
NEUROBLASTS
AND
IMMATURE
NEURONS
.......................................................................................................
13
SOX4/11
CKO
RESULTS
IN
A
DECREASE
OF
DCX/NEUROD1
EXPRESSING
NEUROBLASTS/IMMATURE
NEURONS
..................................................................................
17
SOX4/11
ABLATION
FROM
NEUROBLASTS
AND
IMMATURE
NEURONS
RESULTS
IN
MASSIVE,
RAPID
CELL
LOSS
UP
UNTIL
THE
THIRD
WEEK
AFTER
BIRTH
......................
18
RETROVIRUS-MEDIATED
DELETION
OF
SOX4/11
IN
THE
SECOND
WEEK
AFTER
BIRTH
RESULTS
IN
CELL
LOSS
................................................................................................
21
SOX4/1
1
ABLATION
DOES
NOT
INCREASE
THE
NUMBER
OF
APOPTOTIC
CELLS
IN
THE
DG
...........................................................................................................................................
23
KNOCKOUT
OF
SOX4/11
IN
DIFFERENTIATED
NSPS
IN
VITRO
INCREASES
APOPTOTIC
CELL
DEATH
...........................................................................................................
23
SOX4/1
1
ABLATION
DOES
NOT
ALTER
THE
EXPRESSION
OF
NB/IMN
TRANSCRIPTIONAL
PROGRAM,
BUT
INTERFERES
WITH
THE
LONG-TERM
FATE
......
25
SOX4/11
ABLATION
IN
NB/IMNS
RESULTS
IN
AN
INCREASE
OF
RGLCS
.......................
28
SOX4/11
ABLATION
IN
NESTIN-EXPRESSING
RGLCS
DOES
NOT
INDUCE
RGLC
AMPLIFICATION
AFTER
ONE
WEEK
.........................................................................................
31
SOX4/11
ABLATION
IN
RGL-NSPCS
INHIBITS
LINEAGE
PROGRESSION
TO
EARLY
NEUROBLASTS
............................................................................................................................
34
DISCUSSION
.....................................................................................................................................
37
80X0
FACTORS
AS
A
CENTRAL
PART
OF
A
PUTATIVE
TRANSCRIPTIONAL
NETWORK
CONTROLLING
SURVIVAL
OF
NBS/IMNS
...............................................................................
38
SOXC
ABLATION
DEFINES
A
DISTINCT
DEVELOPMENTAL
STAGE
IN
ADULT
BORN
NEURON
MATURATION
..............................................................................................................
41
SOXC
FACTORS
INDIRECTLY
REGULATE
THE
SIZE
OF
RGLC
POOL
.............................
42
SOXCS
CONTROL
EARLY
NEUROBLAST
GENERATION
.....................................................
44
REGULATION
OF
SOXC
EXPRESSION
AND
ACTIVITY
.........................................................
45
SOXC
FACTOR
IMPLICATION
IN
NEUROPSYCHIATRIC
DISEASE
....................................
46
CONCLUSION
................................................................................................................................
47
OUTLOOK
AND
OPEN
QUESTIONS
.........................................................................................
50
MATERIAL
AND
METHODS
...........................................................................................................
52
MATERIAL
......................................................................................................................................
52
CHEMICALS
AND
REAGENTS
...........................................................................................................
52
BUFFERS
,
SOLUTIONS
AND
MEDIA
...................................................................................................
55
ENZYMES
AND
ENZYME
BUFFERS
..................................................................................................
58
ANTIBODIES
..................................................................................................................................
59
PRIMERS
........................................................................................................................................
59
VIRUSES
AND
PLASMIDS
...............................................................................................................
60
ORGANISMS
..................................................................................................................................
61
EUCARYOTIC
CELLS
.........................................................................................................................
62
KITS
..............................................................................................................................................
62
CONSUMABLE
MATERIAL
................................................................................................................
62
METHODS
..........................................................................................................................................
65
IN
VIVO
ANALYSIS
........................................................................................................................
65
ANIMALS
HOUSING
........................................................................................................................
65
GENERATION
OF
TRANSGENIC
MOUSE
LINES
....................................................................................
65
TAMOXIFEN
TREATMENT.
.................................................................................................................
70
BRDU
TREATMENT
...........................................................................................................................
70
STEREOTACTIC
VIRUS
INJECTIONS
.....................................................................................................
70
TISSUE
PREPARATION
AND
DISSECTION
...........................................................................................
70
IMMUNOHISTOFLUORESCENCE
........................................................................................................
71
IMAGING
........................................................................................................................................
72
CONFOCAL
IMAGE
ANALYSIS
...........................................................................................................
72
IMAGE
PROCESSING
.......................................................................................................................
72
RETRO-/ADENO-VIRUS
METHODOLOGY
................................................................................73
1F8
CELL
CULTURING
........................................................................................................................
73
VIRUS
PRODUCTION
.........................................................................................................................
74
RETRO-
(CAG)
AND
ADENO-
(AD)
VIRUSES
USED
IN
THIS
STUDY.
...................................................
74
VIRUS
PARADIGMS
..........................................................................................................................
75
ADENOVIRUS
..................................................................................................................................
75
MOLECULAR
BIOLOGY
...............................................................................................................
75
GEL
ELECTROPHORESIS
....................................................................................................................
75
TRANSFORMATION
OF
E.COLI
BY
HEAT-SHOCK
TRANSFORMATION
........................................................
75
MINI/MAXI
PREPARATION
................................................................................................................
76
IN
VITRO
ANALYSIS
......................................................................................................................
76
STANDARD
CELL
CULTIVATION
CONDITIONS
........................................................................................
76
CELL
NUMBER
DETERMINATION
.......................................................................................................
76
SPLITTING
OF
ADHERENT
HEK293T
CELLS
........................................................................................
77
TRANSIENT
TRANSFECTION
OF
HEK293T
CELLS
................................................................................77
PRIMARY
MURINE
ADULT
NEURAL
STEM
CELLS
................................................................ 77
ISOLATION
OF
PRIMARY
MURINE
ADULT
NEURAL
STEM
CELLS
..............................................................
77
CULTURING
OF
PRIMARY
MURINE
ADULT
NEURAL
STEM
CELLS
.............................................................
77
SPLITTING
OF
PRIMARY
MURINE
ADULT
NEURAL
STEM
CELLS
..............................................................
77
VIRAL
TRANSDUCTION
........................................................................................................................
78
HTN-CRE
TRANSDUCTION
...............................................................................................................
78
IMMUNOCYTOCHEMISTRY
...............................................................................................................
79
IMMUNOCYTOCHEMICAL
ANALYSIS
OF
APOPTOTIC
CELL
DEATH
................................79
IMAGING
.........................................................................................................................................
79
AUTOMATIC
DETERMINATION
OF
CCASP3
EXPRESSING
CELL
NUMBER
...........................................
79
STATISTICS
....................................................................................................................................
80
LITERATURE
......................................................................................................................................
81
|
| any_adam_object | 1 |
| author | Häberle, Benjamin |
| author_GND | (DE-588)119816915X |
| author_facet | Häberle, Benjamin |
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| id | DE-604.BV046225643 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:38:49Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031604232 |
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| physical | VI, 91 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2019 |
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| record_format | marc |
| spelling | Häberle, Benjamin Verfasser (DE-588)119816915X aut Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis vorgelegt von Benjamin Martin Häberle Erlangen ; Nürnberg [2019] VI, 91 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2019 Gyrus dentatus (DE-588)4390857-3 gnd rswk-swf Entwicklung (DE-588)4113450-3 gnd rswk-swf Neuroblast (DE-588)4832701-3 gnd rswk-swf Hippocampus (DE-588)4642127-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Neuroblast (DE-588)4832701-3 s Entwicklung (DE-588)4113450-3 s Hippocampus (DE-588)4642127-0 s Gyrus dentatus (DE-588)4390857-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031604232&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Häberle, Benjamin Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis Gyrus dentatus (DE-588)4390857-3 gnd Entwicklung (DE-588)4113450-3 gnd Neuroblast (DE-588)4832701-3 gnd Hippocampus (DE-588)4642127-0 gnd |
| subject_GND | (DE-588)4390857-3 (DE-588)4113450-3 (DE-588)4832701-3 (DE-588)4642127-0 (DE-588)4113937-9 |
| title | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis |
| title_auth | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis |
| title_exact_search | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis |
| title_full | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis vorgelegt von Benjamin Martin Häberle |
| title_fullStr | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis vorgelegt von Benjamin Martin Häberle |
| title_full_unstemmed | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis vorgelegt von Benjamin Martin Häberle |
| title_short | Role of SoxC factors in neuroblast, immature neuronal development during adult hippocampal neurogenesis |
| title_sort | role of soxc factors in neuroblast immature neuronal development during adult hippocampal neurogenesis |
| topic | Gyrus dentatus (DE-588)4390857-3 gnd Entwicklung (DE-588)4113450-3 gnd Neuroblast (DE-588)4832701-3 gnd Hippocampus (DE-588)4642127-0 gnd |
| topic_facet | Gyrus dentatus Entwicklung Neuroblast Hippocampus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031604232&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT haberlebenjamin roleofsoxcfactorsinneuroblastimmatureneuronaldevelopmentduringadulthippocampalneurogenesis |