Phospholipid-mediated calcium homeostasis in Toxoplasma gondii:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | x, 77 Seiten Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Kuchipudi, Arunakar |d 1987- |e Verfasser |0 (DE-588)1193941733 |4 aut | |
| 245 | 1 | 0 | |a Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |c von M.Sc. Arunakar Kuchipudi |
| 264 | 1 | |a Berlin |c 2019 | |
| 300 | |a x, 77 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Humboldt-Universität zu Berlin |d 2019 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031587742 | ||
Record in the Search Index
| _version_ | 1804180598590275584 |
|---|---|
| adam_text | III.
TABLE
OF
CONTENTS
I.
ABSTRACT
II.
ZUSAMMENFASSUNG
III.
TABLE
OF
CONTENTS
IV.
LIST
OF
FIGURES
V.
LIST
OF
TABLES
VI.
LIST
OF
ABBREVIATIONS
1.
INTRODUCTION
1.1
APICOMPLEXA
....................
1.1.1
TOXOPLASMA
GONDII
1.2
LYTIC
CYCLE
OF
T.
GONDII
1.3 CALCIUM
SIGNALING
IN
T.
GONDII
1.4
BIOGENESIS
OF
PHOSPHOLIPIDS
1.4.1
PHOSPHOLIPIDS
IN
SIGNALING
1.4.2
PTDTHR
IN
COCCIDIAN
PARASITES
1.5
OBJECTIVES
......................................................................
2.
MATERIALS
AND
METHODS
2.1
MATERIALS
2.1.1
BIOLOGICAL
RESOURCES
2.1.2
T.
GONDII
STRAINS
2.1.3
PLASMIDS/CONSTRUCTS
2.1.4
ANTIBODIES
2.1.5
ENZYMES
2.1.6
OLIGONUCLEOTIDES
2.1.7
CHEMICAL
REAGENTS
2.1.8
BUFFERS
AND
MEDIA
2.1.9
INSTRUMENTS
2.1.10
PLASTIC
WARE
AND
OTHER
DISPOSABLES
2.1.11
COMMERCIAL
KITS
2.1.12
SOFTWARES
2.2
METHODS-
HOST
CELL
AND
PARASITE
CULTIVATION
2.2.1
PROPAGATION
OF
HUMAN
FORESKIN
FIBROBLASTS
2.2.2
CULTURE
AND
PREPARATION
OF
EXTRACELLULAR
PARASITES
2.2.3
TRANSFECTION
OF
TACHYZOITES
2.2.4
CLONAL
DILUTION
2.2.5
TACHYZOITES
EXPRESSING
GENE-ENCODED
CALCIUM
INDICATORS
2.3
METHODS-
MOLECULAR
CLONING
2.3.1
NUCLEIC
ACID
PREPARATION
2.3.2
PCR
REACTIONS
2.3.3
DNA
LIGATION
2.3.4
PREPARATION
OF
CHEMICALLY-COMPETENT
E.
COLI
2.3.5
TRANSFORMATION
OF
ESCHERICHIA
COLI
2.4
METHODS-ASSAYS
...............................
2.4.1
INDIRECT
IMMUNOFLUORESCENCE
ASSAY
2.4.2
PLAQUE
ASSAY
2.4.3
MOTILITY
ASSAY
2.4.4
INVASION
AND
EGRESS
ASSAYS
2.4.5
REPLICATION
ASSAY
2.4.6
SDS-PAGE
AND
WESTERN
BLOT
2.4.7
LIPID
EXTRACTION
AND
SEPARATION
.........................................................................
22
2.4.8
PREPARATION
OF
GLASS
BEADS
FOR
LIPID
BINDING
ASSAY
............................................
23
2.4.9
PROTEIN-LIPID
OVERLAY
...........................................................................................
23
2.4.10
CALCIUM
MEASUREMENTS
....................................................................................
24
2.4.11
EXPRESSION
OF
RECOMBINANT
PROTEINS
...............................................................
24
2.4.12
STATISTICAL
ANALYSIS
.............................................................................................
25
3.
RESULTS.........................................................................................................
26
3.1
PTDTHR
REGULATES
CALCIUM
HOMEOSTASIS
IN
T.
GONDII
..........................................
26
3.1.1
EXPRESSION
OF
INTENSIOMETRIC
GECI
FOR
CALCIUM
MEASUREMENTS
......................
26
3.1.2
GCAMPSS
IS
NOT
DETRIMENTAL
TO
THE
PARASITE
GROWTH
AND
REPLICATION
..............
27
3.1.3
GCAMPSS
RESPONDS
TO
CHANGES
IN
THE
CYTOSOLIC
CALCIUM
...................................
29
3.1.4
THE
A
TGPTS
MUTANT
SHOWS
A
COMPROMISED
CALCIUM
FLUX
DURING
EGRESS
..........
30
3.1.5
EXPRESSION
OF
RATIOMETRIC
GECI
FOR
CALCIUM
IMAGING..................
33
3.1.6
ECTOPIC
EXPRESSION
OF
ER-TYPE
SERCA
RESCUES
THE
A
TGPTS
MUTANT
..................
35
3.2
POTENTIAL
MECHANISM
OF
LIPID-REGULATED
CALCIUM
HOMEOSTASIS
IN
T.
GONDII
..
37
3.2.1
C2
DOMAIN-CONTAINING
PROTEINS
..........................................................................
37
3.2.2
LIPID-BINDING
PROTEINS
IN
T.
GONDII
.....................................................................
38
3.3
LACT-C2,
A
KNOWN
PTDSER
BIOSENSOR,
CAN
ALSO
BIND
TO
PTDTHR
..........................
40
4.
DISCUSSION
...................................................................................................
44
4.1
CALCIUM
DETECTION
IN
T.
GONDII
...............................................................................
45
4.2
EVALUATION
OF
GCAMP6S
IN
T.
GONDII
......................................................................
46
4.3
CALCIUM
MEASUREMENTS
..........................................................................................
47
4.4
LIPID-BINDING
PROTEINS
AND
CALCIUM
HOMEOSTASIS
.......... ..........
48
4.5
DISTRIBUTION
OF
PTDTHR
IN
TACHYZOITES
USING
LIPID
BIOSENSORS
.........................
50
5.
CONCLUSION
AND
OUTLOOK
..............................................................................
53
VII.
REFERENCES
................................................................................................
54
VIII.
APPENDICES
.................
62
IX.
LIST
OF
PUBLICATIONS
..................................................................................
75
X.
ACKNOWLEDGEMENTS
....................................................................................
76
XI.
ERKLARUNG
..................................................................................................
77
|
| any_adam_object | 1 |
| author | Kuchipudi, Arunakar 1987- |
| author_GND | (DE-588)1193941733 |
| author_facet | Kuchipudi, Arunakar 1987- |
| author_role | aut |
| author_sort | Kuchipudi, Arunakar 1987- |
| author_variant | a k ak |
| building | Verbundindex |
| bvnumber | BV046208804 |
| classification_rvk | XD 9304 XD 9312 XD 9321 YD 9404 YD 9412 |
| ctrlnum | (OCoLC)1125186599 (DE-599)DNB1192996801 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV046208804 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:38:19Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031587742 |
| oclc_num | 1125186599 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-11 |
| owner_facet | DE-91 DE-BY-TUM DE-11 |
| physical | x, 77 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Kuchipudi, Arunakar 1987- Verfasser (DE-588)1193941733 aut Phospholipid-mediated calcium homeostasis in Toxoplasma gondii von M.Sc. Arunakar Kuchipudi Berlin 2019 x, 77 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Humboldt-Universität zu Berlin 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1192996801/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031587742&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kuchipudi, Arunakar 1987- Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |
| subject_GND | (DE-588)4113937-9 |
| title | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |
| title_auth | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |
| title_exact_search | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |
| title_full | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii von M.Sc. Arunakar Kuchipudi |
| title_fullStr | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii von M.Sc. Arunakar Kuchipudi |
| title_full_unstemmed | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii von M.Sc. Arunakar Kuchipudi |
| title_short | Phospholipid-mediated calcium homeostasis in Toxoplasma gondii |
| title_sort | phospholipid mediated calcium homeostasis in toxoplasma gondii |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1192996801/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031587742&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kuchipudiarunakar phospholipidmediatedcalciumhomeostasisintoxoplasmagondii |
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