Rapid Cycle Real-Time PCR — Methods and Applications: Quantification
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum m...
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| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2004
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| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybrdidization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier! |
| Beschreibung: | 1 Online-Ressource (VII, 223 p. 526 illus., 511 illus. in color) |
| ISBN: | 9783642188404 |
| DOI: | 10.1007/978-3-642-18840-4 |
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Datensatz im Suchindex
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| discipline | Biologie |
| doi_str_mv | 10.1007/978-3-642-18840-4 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:36:34Z |
| institution | BVB |
| isbn | 9783642188404 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031528751 |
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| physical | 1 Online-Ressource (VII, 223 p. 526 illus., 511 illus. in color) |
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| publisher | Springer Berlin Heidelberg |
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| spelling | Rapid Cycle Real-Time PCR — Methods and Applications Quantification edited by Carl Wittwer, Meinhard Hahn, Karen Kaul Berlin, Heidelberg Springer Berlin Heidelberg 2004 1 Online-Ressource (VII, 223 p. 526 illus., 511 illus. in color) txt rdacontent c rdamedia cr rdacarrier Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybrdidization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier! Biochemistry, general Biochemistry Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Wittwer, Carl edt Hahn, Meinhard edt Kaul, Karen edt Erscheint auch als Druck-Ausgabe 9783540206293 Erscheint auch als Druck-Ausgabe 9783642623172 Erscheint auch als Druck-Ausgabe 9783642188411 https://doi.org/10.1007/978-3-642-18840-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Rapid Cycle Real-Time PCR — Methods and Applications Quantification Biochemistry, general Biochemistry Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4143413-4 |
| title | Rapid Cycle Real-Time PCR — Methods and Applications Quantification |
| title_auth | Rapid Cycle Real-Time PCR — Methods and Applications Quantification |
| title_exact_search | Rapid Cycle Real-Time PCR — Methods and Applications Quantification |
| title_full | Rapid Cycle Real-Time PCR — Methods and Applications Quantification edited by Carl Wittwer, Meinhard Hahn, Karen Kaul |
| title_fullStr | Rapid Cycle Real-Time PCR — Methods and Applications Quantification edited by Carl Wittwer, Meinhard Hahn, Karen Kaul |
| title_full_unstemmed | Rapid Cycle Real-Time PCR — Methods and Applications Quantification edited by Carl Wittwer, Meinhard Hahn, Karen Kaul |
| title_short | Rapid Cycle Real-Time PCR — Methods and Applications |
| title_sort | rapid cycle real time pcr methods and applications quantification |
| title_sub | Quantification |
| topic | Biochemistry, general Biochemistry Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Biochemistry, general Biochemistry Polymerase-Kettenreaktion Aufsatzsammlung |
| url | https://doi.org/10.1007/978-3-642-18840-4 |
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