Tissue Culture Techniques: An Introduction
ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . ....
Gespeichert in:
1. Verfasser: | |
---|---|
Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Boston, MA
Birkhäuser Boston
1994
|
Schlagworte: | |
Online-Zugang: | UBR01 Volltext |
Zusammenfassung: | ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations - Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat - Radiation - Toxic gas - Filtration - Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions - water as a reagent - Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells - Common enzyme solutions - Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer - Particle counter - Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi - Fungi - Mycoplasma - Viruses - Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth - Recurrent contamination - When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards - Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum - Treatments of serum - Plasma-derived serum - Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
Beschreibung: | 1 Online-Ressource (260 p) |
ISBN: | 9781461202479 |
DOI: | 10.1007/978-1-4612-0247-9 |
Internformat
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520 | |a ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations - Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat - Radiation - Toxic gas - Filtration - Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . | ||
520 | |a . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions - water as a reagent - Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells - Common enzyme solutions - Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer - Particle counter - Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi - Fungi - Mycoplasma - Viruses - Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | ||
520 | |a 73 Inadequate cell growth - Recurrent contamination - When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards - Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum - Treatments of serum - Plasma-derived serum - Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | ||
650 | 4 | |a General Practice / Family Medicine | |
650 | 4 | |a Family medicine | |
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Datensatz im Suchindex
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---|---|
any_adam_object | |
author | Martin, Bernice M. |
author_facet | Martin, Bernice M. |
author_role | aut |
author_sort | Martin, Bernice M. |
author_variant | b m m bm bmm |
building | Verbundindex |
bvnumber | BV046146314 |
classification_rvk | WX 6600 |
collection | ZDB-2-SME |
ctrlnum | (ZDB-2-SME)978-1-4612-0247-9 (OCoLC)1119148269 (DE-599)BVBBV046146314 |
dewey-full | 610 |
dewey-hundreds | 600 - Technology (Applied sciences) |
dewey-ones | 610 - Medicine and health |
dewey-raw | 610 |
dewey-search | 610 |
dewey-sort | 3610 |
dewey-tens | 610 - Medicine and health |
discipline | Biologie Medizin |
doi_str_mv | 10.1007/978-1-4612-0247-9 |
format | Electronic eBook |
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id | DE-604.BV046146314 |
illustrated | Not Illustrated |
indexdate | 2024-07-10T08:36:29Z |
institution | BVB |
isbn | 9781461202479 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031526499 |
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physical | 1 Online-Ressource (260 p) |
psigel | ZDB-2-SME ZDB-2-SME_1990/2004 ZDB-2-SME ZDB-2-SME_1990/2004 |
publishDate | 1994 |
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publisher | Birkhäuser Boston |
record_format | marc |
spelling | Martin, Bernice M. Verfasser aut Tissue Culture Techniques An Introduction by Bernice M. Martin Boston, MA Birkhäuser Boston 1994 1 Online-Ressource (260 p) txt rdacontent c rdamedia cr rdacarrier ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations - Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat - Radiation - Toxic gas - Filtration - Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions - water as a reagent - Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells - Common enzyme solutions - Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer - Particle counter - Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi - Fungi - Mycoplasma - Viruses - Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth - Recurrent contamination - When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards - Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum - Treatments of serum - Plasma-derived serum - Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . General Practice / Family Medicine Family medicine Zellkultur (DE-588)4067547-6 gnd rswk-swf Gewebekultur (DE-588)4157245-2 gnd rswk-swf Zellkultur (DE-588)4067547-6 s DE-604 Gewebekultur (DE-588)4157245-2 s Erscheint auch als Druck-Ausgabe 9780817637187 Erscheint auch als Druck-Ausgabe 9780817636432 Erscheint auch als Druck-Ausgabe 9781461202486 https://doi.org/10.1007/978-1-4612-0247-9 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Martin, Bernice M. Tissue Culture Techniques An Introduction General Practice / Family Medicine Family medicine Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd |
subject_GND | (DE-588)4067547-6 (DE-588)4157245-2 |
title | Tissue Culture Techniques An Introduction |
title_auth | Tissue Culture Techniques An Introduction |
title_exact_search | Tissue Culture Techniques An Introduction |
title_full | Tissue Culture Techniques An Introduction by Bernice M. Martin |
title_fullStr | Tissue Culture Techniques An Introduction by Bernice M. Martin |
title_full_unstemmed | Tissue Culture Techniques An Introduction by Bernice M. Martin |
title_short | Tissue Culture Techniques |
title_sort | tissue culture techniques an introduction |
title_sub | An Introduction |
topic | General Practice / Family Medicine Family medicine Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd |
topic_facet | General Practice / Family Medicine Family medicine Zellkultur Gewebekultur |
url | https://doi.org/10.1007/978-1-4612-0247-9 |
work_keys_str_mv | AT martinbernicem tissueculturetechniquesanintroduction |