Methods in DNA Amplification:
The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'th...
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| Other Authors: | , , |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Boston, MA
Springer US
1994
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| Subjects: | |
| Online Access: | UBR01 Volltext |
| Summary: | The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure |
| Physical Description: | 1 Online-Ressource (IX, 251 p) |
| ISBN: | 9781461525301 |
| DOI: | 10.1007/978-1-4615-2530-1 |
Staff View
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| 520 | |a The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure | ||
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| dewey-full | 616.9 |
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| discipline | Medizin |
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| format | Electronic eBook |
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| id | DE-604.BV046144394 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:36:25Z |
| institution | BVB |
| isbn | 9781461525301 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031524579 |
| oclc_num | 1119076670 |
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| physical | 1 Online-Ressource (IX, 251 p) |
| psigel | ZDB-2-SME ZDB-2-SME_1990/2004 ZDB-2-SME ZDB-2-SME_1990/2004 |
| publishDate | 1994 |
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| publisher | Springer US |
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| spelling | Methods in DNA Amplification edited by Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh Proceedings of the Second International PCR Symposium on Usage of PCR and Alternative Amplification Methods in Infectious and Genetic Diseases held in Berlin, Germany, February 26-27, 1993 Boston, MA Springer US 1994 1 Online-Ressource (IX, 251 p) txt rdacontent c rdamedia cr rdacarrier The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure Infectious Diseases Cell Biology Animal Anatomy / Morphology / Histology Plant Sciences Emerging infectious diseases Cytology Morphology (Animals) Botany Rolfs, Arndt edt Weber-Rolfs, Ines edt Finckh, Ulrich edt Erscheint auch als Druck-Ausgabe 9780306449086 Erscheint auch als Druck-Ausgabe 9781461360780 Erscheint auch als Druck-Ausgabe 9781461525318 https://doi.org/10.1007/978-1-4615-2530-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Methods in DNA Amplification Infectious Diseases Cell Biology Animal Anatomy / Morphology / Histology Plant Sciences Emerging infectious diseases Cytology Morphology (Animals) Botany |
| title | Methods in DNA Amplification |
| title_alt | Proceedings of the Second International PCR Symposium on Usage of PCR and Alternative Amplification Methods in Infectious and Genetic Diseases held in Berlin, Germany, February 26-27, 1993 |
| title_auth | Methods in DNA Amplification |
| title_exact_search | Methods in DNA Amplification |
| title_full | Methods in DNA Amplification edited by Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh |
| title_fullStr | Methods in DNA Amplification edited by Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh |
| title_full_unstemmed | Methods in DNA Amplification edited by Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh |
| title_short | Methods in DNA Amplification |
| title_sort | methods in dna amplification |
| topic | Infectious Diseases Cell Biology Animal Anatomy / Morphology / Histology Plant Sciences Emerging infectious diseases Cytology Morphology (Animals) Botany |
| topic_facet | Infectious Diseases Cell Biology Animal Anatomy / Morphology / Histology Plant Sciences Emerging infectious diseases Cytology Morphology (Animals) Botany |
| url | https://doi.org/10.1007/978-1-4615-2530-1 |
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