Die Rolle von ATF4 bei Hirntumoren: Analyse der Angiogenese, Chemoresistenz und Ferroptose
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | German English |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2019]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | Enthält Sonderabdrucke |
| Beschreibung: | 75 Seiten Illustrationen, Diagramme 21 cm |
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| 245 | 1 | 0 | |a Die Rolle von ATF4 bei Hirntumoren |b Analyse der Angiogenese, Chemoresistenz und Ferroptose |c vorgelegt von Daishi Chen |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2019] | |
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| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
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Datensatz im Suchindex
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| adam_text | TABLE
OF
CONTENTS
SUMMARY...........................................................................................................................................
1
ZUSAMMENFASSUNG
...........................................................................................................................
3
INTRODUCTION
......................................................................................................................................
5
EINFUEHRUNG
......................................................................................................................................
15
PUBLICATION
ARTICLE
..........................................................................................................................
27
ABSTRACT
.......................................................................................................................................
27
INTRODUCTION
................................................................................................................................
27
RESULTS
.........................................................................................................................................
28
TMZ
UP-REGULATES
ACTIVATING
TRANSCRIPTION
FACTOR
4
(ATF4)
AND
XCT
IN
GLIOMA
CELLS
.............
28
ATF4
EXPRESSION
LEVELS
CORRELATE
WITH
TMZ
RESISTANCE
.........................................................
28
IMPACT
OF
ATF4
ON
TMZ-INDUCED
CELL
DEATH
..........................................................................
28
EFFECTS
OF
ELEVATED
ATF4
ON
TMZ-INDUCED
62/M
ARREST
IN
GLIOMA
CELLS
...............................
30
ATF4
PROMOTES
GLIOMA
CELL
MIGRATION
....................................................................................
30
ATF4
MODULATES
THE
AMINO
ACIDS
SECRETION
OF
GLIOMAS
........................................................
30
DISCUSSION...................................................................................................................................
30
MATERIAL
AND
METHODS
...............................................................................................................
36
CELL
CULTURE
............................................................................................................................
36
CHEMICALS
...............................................................................................................................
36
CONSTRUCTION
OF
PLASMIDS
CARRYING
GENES
OF
THE
TARGET
PROTEINS
.........................................
36
CONSTRUCTION
OF
SHRNA
PLASMIDS
...........................................................................................
36
CELL
TRANSFECTION
.....................................................................................................................
36
CELL
VIABILITY
ASSAY
..................................................................................................................
36
REAL
TIME
PCR
ANALYSIS...........................................................................................................
36
WESTERN
BLOTTING
....................................................................................................................
37
IMMUNOFLUORESCENCE
STAINING
...............................................................................................
37
THREE
DIMENSIONAL
SPHEROID
MIGRATION
ASSAY
.......................................................................
37
CELL
DEATH
ANALYSIS
BY
7-AAD
AND
ANNEXIN
V
ASSAY
...............................................................
37
CELL
CYCLE
ANALYSIS.................................................................................................................
37
METABOLIE
ASSAYS
....................................................................................................................
37
STATISTICAL
ANALYSIS
..................................................................................................................
37
ACKNOWLEDGMENTS
......................................................................................................................
38
REFERENCES..................................................................................................................................
38
PUBLICATION
ARTICLE
..........................................................................................................................41
ABSTRACT
.......................................................................................................................................
42
INTRODUCTION
................................................................................................................................
42
RESULTS
.........................................................................................................................................
43
ATF4
FOSTERS
TUMOR
CELL
GROWTH
.............................................................................................
43
ATF4
REGULATES
XCT
EXPRESSION
AND
GLUTAMATE
SECRETION
......................................................
43
ATF4
EXPRESSION
INDUCES
ACQUIRED
CELL
DEATH
RESISTANCE
......................................................
43
ATF4
KNOCKDOWN
RENDERS
CELLS
SUSCEPTIBLE
FOR
FERROPTOSIS
...................................................
45
ATF4
OVEREXPRESSION
INCREASES
NEURONAL
DAMAGE
THROUGH
ELEVATED
XCT
EXPRESSION
..........
45
ATF4
PROMOTES
TUMOR
ANGIOGENESIS
......................................................................................
51
DISCUSSION...................................................................................................................................
51
MATERIAL
AND
METHODS
..............................................................................................................
53
CELL
CULTURE
..............................................................................................................................
53
CHEMICALS
AND
REAGENTS
.........................................................................................................
55
DATABASE
ANALYSIS
..................................................................................................................
55
CONSTRUCTION
OF
EXPRESSION
PLASMIDS
AND
CELL
CULTURING
.......................................................
55
CONSTRUCTION
OF
SHRNA
PLASMIDS
AND
CELL
TRANSFECTION
.........................................................
55
CELL
VIABILITY
ASSAYS
AND
INHIBITOR
STUDIES
.............................................................................
55
COLONY
FORMING
ASSAY
.............................................................................................................
55
CELL
MORPHOLOGY
ANALYSIS.......................................................................................................
55
REAL
TIME
RT-PCR
ANALYSIS......................................................................................................
55
IMMUNOBLOTTING
......................................................................................................................
55
THREE
DIMENSIONAL
SPHEROID
MIGRATION
ASSAY
.......................................................................
55
CELL
CYCLE
ANALYSIS..................................................................................................................
56
BRDU
INCORPORATION
ASSAY
.....................................................................................................
56
56
CELL
DEATH
ANALYSIS
BY
7-AAD
AND
ANNEXIN
V
ASSAY
STAINING
FOR
LIPID
PEROXIDATION
IN
CELLS
BY
FLOW
CYTOMETRY
56
METABOLIC
PROFILING
OF
TUMOR
CELLS
56
VOGIM
ASSAY
AND
ANGIOGENESIS
ANALYSIS
56
STATISTICAL
ANALYSIS 56
PATIENT
SAMPLES
56
ETHICAL
STATEMENTS
56
ACKNOWLEDGMENTS
56
REFERENCES
57
PUBLICATION
ARTICLE.
ABSTRACT.
59
INTRODUCTION.
59
MATERIALS.
BUFFERS
AND
SOLUTIONS.
60
FLUORESCENCE-ACTIVATED
CELL-SORTING
DEVICE
62
CELLS
AND
CELL
CULTURE
CONDITIONS
62
METHODS
62
EXPERIMENTAL
PROCEDURE
62
STAINING
PROCEDURE
FOR
FITC
ANNEXIN
V
AND
7-AAD
62
FERROPTOSIS
AND
CELL
DEATH
INDUCTION
PROCEDURE
63
REFERENCES
65
REFERENCES
67
ACKNOWLEDGMENTS
73
LIST
OF
PUBLICATION
74
|
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| author | Chen, Daishi |
| author_facet | Chen, Daishi |
| author_role | aut |
| author_sort | Chen, Daishi |
| author_variant | d c dc |
| building | Verbundindex |
| bvnumber | BV046116213 |
| ctrlnum | (OCoLC)1118986608 (DE-599)DNB1190333570 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T08:35:42Z |
| institution | BVB |
| language | German English |
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| spelling | Chen, Daishi Verfasser aut Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose vorgelegt von Daishi Chen Erlangen ; Nürnberg [2019] 75 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Enthält Sonderabdrucke Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2019 1\p Arzneimittelresistenz (DE-588)4143180-7 gnd 2\p Zelle (DE-588)4067537-3 gnd 3\p Hirntumor (DE-588)4072521-2 gnd 4\p Angiogenese (DE-588)4142449-9 gnd (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1190333570/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031496711&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p maschinell gebildet 0,02853 20190717 DE-101 2\p maschinell gebildet 0,02721 20190717 DE-101 3\p maschinell gebildet 0,02555 20190717 DE-101 4\p maschinell gebildet 0,01703 20190717 DE-101 |
| spellingShingle | Chen, Daishi Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose 1\p Arzneimittelresistenz (DE-588)4143180-7 gnd 2\p Zelle (DE-588)4067537-3 gnd 3\p Hirntumor (DE-588)4072521-2 gnd 4\p Angiogenese (DE-588)4142449-9 gnd |
| subject_GND | (DE-588)4143180-7 (DE-588)4067537-3 (DE-588)4072521-2 (DE-588)4142449-9 (DE-588)4113937-9 |
| title | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose |
| title_auth | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose |
| title_exact_search | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose |
| title_full | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose vorgelegt von Daishi Chen |
| title_fullStr | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose vorgelegt von Daishi Chen |
| title_full_unstemmed | Die Rolle von ATF4 bei Hirntumoren Analyse der Angiogenese, Chemoresistenz und Ferroptose vorgelegt von Daishi Chen |
| title_short | Die Rolle von ATF4 bei Hirntumoren |
| title_sort | die rolle von atf4 bei hirntumoren analyse der angiogenese chemoresistenz und ferroptose |
| title_sub | Analyse der Angiogenese, Chemoresistenz und Ferroptose |
| topic | 1\p Arzneimittelresistenz (DE-588)4143180-7 gnd 2\p Zelle (DE-588)4067537-3 gnd 3\p Hirntumor (DE-588)4072521-2 gnd 4\p Angiogenese (DE-588)4142449-9 gnd |
| topic_facet | Arzneimittelresistenz Zelle Hirntumor Angiogenese Hochschulschrift |
| url | http://d-nb.info/1190333570/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031496711&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT chendaishi dierollevonatf4beihirntumorenanalysederangiogenesechemoresistenzundferroptose |
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