Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | German |
| Published: |
Ulm
Juli 2018
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 154 Seiten Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Hahn, Tobias Michael |d 1988- |e Verfasser |0 (DE-588)1177832585 |4 aut | |
| 245 | 1 | 0 | |a Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum |c vorgelegt von Tobias Michael Hahn aus Riedlingen |
| 264 | 1 | |a Ulm |c Juli 2018 | |
| 300 | |a 154 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 502 | |b Dissertation |c Universität Ulm |d 2018 | ||
| 546 | |a Zusammenfassung in englischer und deutscher Sprache | ||
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| 650 | 0 | 7 | |a Ribosomale RNS |0 (DE-588)4076715-2 |2 gnd |9 rswk-swf |
| 653 | |a Corynebacterium glutamicum | ||
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Record in the Search Index
| _version_ | 1804180292191125504 |
|---|---|
| adam_text | INHALT
ZUSAMMENFASSUNG
...............................................................................................................................
1
SUMMARY
...............................................................................................................................................
3
1.
EINLEITUNG
.......................................................................................................................................
5
2.
MATERIAL
&
METHODEN
...............................................................................................................
11
2.1
BAKTERIENSTAEMME,
PLASMIDE
UND
OLIGONUKLEOTIDE
................................................................
11
2.2
CHEMIKALIEN,
ENZYME,
KITS,
MATERIALIEN
UND
GERAETE
.............................................................
17
2.3
NAEHRMEDIEN,
STAMMHALTUNG
UND
KULTIVIERUNGSBEDINGUNGEN...............................................
26
2.4
ARBEITEN
MIT
NUKLEINSAEUREN
..................................................................................................
29
2.4.1
TRANSFORMATION
VON
PLASMID-DNA
.................................................................................
29
2.4.2
ISOLIERUNG
UND
KONZENTRATIONSBESTIMMUNG
VON
NUKLEINSAEUREN...................................
31
2.4.3
SEQUENZIERUNG
VON
DNA
................................................................................................
33
2.4.4
AMPLIFIKATION
VON
DNA-FRAGMENTEN.............................................................................
34
2.4.5
AUFTRENNUNG
VON
DNA-FRAGMENTEN..............................................................................
41
2.4.6
REINIGUNG
VON
PCR-PRODUKTEN
......................................................................................
42
2.4.7
ENZYMATISCHE
MODIFIKATION
VON
DESOXYRIBONUKLEINSAEUREN
..........................................
42
2.5
ARBEITEN
MIT
PROTEINEN
........................................................................................................
43
2.5.1
BESTIMMUNG
DER
CHLORAMPHENICOL-ACETYLTRANSFERASE-AKTIVITAET
(CAT-ASSAY)
...............
43
2.5.2
PROTEINBESTIMMUNG
MIT
DEM
*BCA
PROTEIN
ASSAY
......................................................
44
2.5.3
FLUORESZENZMESSUNG
VON
EMGFP
.................................................................................
45
2.6
DNA-AFFINITAETSCHROMATOGRAPHIE
..........................................................................................
46
3.
ERGEBNISSE
..................................................................................................................................
50
3.1
KONSTRUKTION
UND
VERIFIZIERUNG
DER
PET2-REPORTERGENPLASMIDE
..........................................
51
3.2
AKTIVITAETEN
DER
RRN-PROMOTOREN
IN
C.
GLUTAMICUM
WT
UND
IN
RRN-DELETIONSMUTANTEN
.......
55
3.3
KOPIENZAHL
DER
PET2-REPORTERGENPLASMIDE.........................................................................
69
3.4
QUANTITATIVE
ANALYSE
DER
COT-REPORTERGENEXPRESSION..........................................................
72
3.5
VERFIKATION
VON
REPORTERGENPLASMIDEN
IN
DER
FUENFFACH-RRN
DELETIONSMUTANTE
C.
GLUTAMICUM
BRRNABCDF
........................................................................................................
74
3.6
RRN-PROMOTORAKTIVITAETEN
IN
INTEGRATIONSMUTANTEN
VON
C.
GLUTAMICUM
WT
UND
RRN-
DELETIONSMUTANTEN
..............................................................................................................
82
3.7
RRNA-PROMOTORAKTIVITAETEN
IN
C.
GLUTAMICUM
WT
UND
C.
GLUTAMICUM
LRRNABCF
MITTELS
DES
REPORTERGENS
EM
GFP
............................................................................................................
89
3.8
IDENTIFIZIERUNG
VON
DNA-BINDENDEN,
POTENTIELLEN
RRN-REGULATORPROTEINEN
VIA
DNA-
AFFINITAETSCHROMATOGRAPHIE
....................................................................................................
92
4.
DISKUSSION
...................................................................................................................................
95
5.
LITERATURVERZEICHNIS
.................................................................................................................113
6.
ABKUERZUNGEN
.............................................................................................................................
129
7.
PUBLIKATIONEN
............................................................................................................................133
8.
DANKSAGUNG
...............................................................................................................................135
9.
ABGRENZUNG
DER
EIGENLEISTUNG
................................................................................................
137
10.
ERKLAERUNG
...............................................................................................................................
139
11.
LEBENSLAUF
.............................................................................................................................141
12.
ANHANG
..................................................................................................................................143
|
| any_adam_object | 1 |
| author | Hahn, Tobias Michael 1988- |
| author_GND | (DE-588)1177832585 |
| author_facet | Hahn, Tobias Michael 1988- |
| author_role | aut |
| author_sort | Hahn, Tobias Michael 1988- |
| author_variant | t m h tm tmh |
| building | Verbundindex |
| bvnumber | BV046036887 |
| ctrlnum | (OCoLC)1108176941 (DE-599)BSZ517908298 |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046036887 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:33:26Z |
| institution | BVB |
| institution_GND | (DE-588)30607-1 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031418644 |
| oclc_num | 1108176941 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T |
| owner_facet | DE-355 DE-BY-UBR DE-29T |
| physical | 154 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| record_format | marc |
| spelling | Hahn, Tobias Michael 1988- Verfasser (DE-588)1177832585 aut Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum vorgelegt von Tobias Michael Hahn aus Riedlingen Ulm Juli 2018 154 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Zusammenfassung in englischer und deutscher Sprache Genexpression (DE-588)4020136-3 gnd rswk-swf Corynebacterium glutamicum (DE-588)4309351-6 gnd rswk-swf Ribosomale RNS (DE-588)4076715-2 gnd rswk-swf Corynebacterium glutamicum Biotechnology RNA (DE-588)4113937-9 Hochschulschrift gnd-content Corynebacterium glutamicum (DE-588)4309351-6 s Genexpression (DE-588)4020136-3 s Ribosomale RNS (DE-588)4076715-2 s DE-604 Universität Ulm (DE-588)30607-1 dgg DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031418644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hahn, Tobias Michael 1988- Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum Genexpression (DE-588)4020136-3 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Ribosomale RNS (DE-588)4076715-2 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4309351-6 (DE-588)4076715-2 (DE-588)4113937-9 |
| title | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum |
| title_auth | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum |
| title_exact_search | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum |
| title_full | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum vorgelegt von Tobias Michael Hahn aus Riedlingen |
| title_fullStr | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum vorgelegt von Tobias Michael Hahn aus Riedlingen |
| title_full_unstemmed | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum vorgelegt von Tobias Michael Hahn aus Riedlingen |
| title_short | Untersuchung der ribosomalen RNA-Expression in Corynebacterium glutamicum |
| title_sort | untersuchung der ribosomalen rna expression in corynebacterium glutamicum |
| topic | Genexpression (DE-588)4020136-3 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Ribosomale RNS (DE-588)4076715-2 gnd |
| topic_facet | Genexpression Corynebacterium glutamicum Ribosomale RNS Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031418644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hahntobiasmichael untersuchungderribosomalenrnaexpressionincorynebacteriumglutamicum AT universitatulm untersuchungderribosomalenrnaexpressionincorynebacteriumglutamicum |