Distinct effects of cAMP effectors on brown adipocytes:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Bonn
2019
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | V, 86 Seiten Illustrationen 21 cm |
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MARC
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| 100 | 1 | |a Reverte Salisa, Laia |e Verfasser |0 (DE-588)1177181657 |4 aut | |
| 245 | 1 | 0 | |a Distinct effects of cAMP effectors on brown adipocytes |c Laia Reverte Salisa |
| 264 | 1 | |a Bonn |c 2019 | |
| 300 | |a V, 86 Seiten |b Illustrationen |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Rheinische Friedrich-Wilhelms-Universität Bonn |d 2018 | ||
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|---|---|
| adam_text | TABLE
OF
CONTENTS
ACKNOWLEDGEMENTS
.....................................................................................................................
I
TABLE
OF
CONTENTS
........................................................................................................................
II
ABBREVIATIONS
............................................................................................................................
IV
1.
INTRODUCTION
.........................................................................................................................
1
1.1.
GPCRS
............................................................................................................................
1
1.2.
CAMP
EFFECTORS:
PKA
AND
EPAC
..................................................................................
3
1.3.
CGMP
AND
ITS
DOWNSTREAM
SIGNALING
............................................................................
6
1.4.
OBESITY
AND
ADIPOSE
TISSUES
..........................................................................................
6
2.
AIM
OF
THE
THESIS
...............................................................................................................
11
3.
METHODS
..............................................................................................................................
12
3.1.
COMMON
CHEMICALS
.........................................................................................................
12
3.2.
ANIMALS
.............................................................................................................................
12
3.2.1.
EPAC1
KO
MICE
................................................................................
12
3.2.2.
EX
VIVO
LIPOLYSIS
.........................................................................................................
15
3.3.
IMMUNOHISTOCHEMISTRY
.....................................................................................................
16
3.3.1.
HEMATOXYLIN/EOSIN
(HE)
STAINING
.............................................................................
16
3.3.2.
UCP1
STAINING
...........................................................................................................
17
3.4.
CELL
CULTURE
........................................................................................................................
17
3.4.1.
CELL
CULTURE
OF
BA
......................................................................................................
19
3.4.2.
CELL
CULTURE
OF
HUMAN
MULTIPOTENT
ADIPOSE-DERIVED
STEM
CELLS
(HMADS)
..................
22
3.4.3.
CELL
CULTURE
OF
WA
.....................................................................................................
24
3.5.
PROLIFERATION
ASSAY
...........................................................................................................26
3.6.
RNA
METHODS
..................................................................................................................
27
3.6.1.
MRNA
ANALYSIS
USING
REAL-TIME
PCR
(QPCR)
.......................................................
28
3.7.
PROTEIN
METHODS
...............................................................................................................
30
3.7.1.
SDS-PAGE
AND
WESTERN
BLOT
..................................................................................
32
3.8.
OIL
RED
0
STAINING
............................................................................................................
35
3.9.
IN
VITRO
LIPOLYSIS
.................................................................................................................
36
3.10.
STATISTICAL
ANALYSIS
.........................................................................................................
37
4.
RESULTS
................................................................................................................................
38
4.1.
DIFFERENTIAL
EFFECTS
OF
CAMP
AND
CGMP
IN
BA
............................................................
38
4.1.1.
CAMP
DOWNREGULATES
MURINE
BA
DIFFERENTIATION
.....................................................
38
4.1.2.
CAMP
STIMULATION
PROMOTES
HUMAN
BROWN
ADIPOCYTE
DIFFERENTIATION
.....................
48
4.2.
DIFFERENTIAL
EFFECTS
OF
CAMP
EFFECTORS
ON
BA
AND
BROWNING
...........................................
53
4.2.1.
SUSTAINED
STIMULATION
OF
THE
CAMP-EPAC
AXIS
INCREASES
BA
DIFFERENTIATION
........
53
4.2.2.
SUSTAINED
STIMULATION
OF
THE
CAMP-EPAC
AXIS
PROMOTES
BROWNING
OF
WA
...........
58
4.3.
ANALYSIS
OF
EP
AC
1
KO
IN
ADIPOCYTES
AND
ADIPOSE
TISSUE
.............................................
61
4.3.1.
EPAC1
LOSS
IN
BA
AND
DOWNSTREAM
SIGNALING
.........................................................
61
4.3.2.
EPAC1
LOSS
DECREASES
WA
DIFFERENTIATION
AND
NE-MEDIATED
BROWNING
................
64
4.3.4.
EPALI
LOSS
IN
NEONATAL
BAT
...................................................................................
65
4.3.5.
LOSS
OF
EPALI
IN
ADULT
ADIPOSE
TISSUES
.................................................................
66
4.4.
EPAC1
LEVELS
IN
DIET-INDUCED
OBESITY
............................................................................
68
5.
DISCUSSION
.........................................................................................................................
69
5.
1.
DIFFERENTIAL
EFFECTS
OFCAMP
AND
CGMP
IN
BA
................................................................
69
5.2.
DIFFERENTIAL
EFFECTS
OFCAMP
EFFECTORS
ON
BA
AND
BROWNING
...........................................
72
5.2.1.
SUSTAINED
STIMULATION
OF
THE
CAMP-EPAC
AXIS
INCREASES
BA
DIFFERENTIATION
.......
72
5.2.2.
SUSTAINED
STIMULATION
OF
THE
CAMP-EPAC
AXIS
PROMOTES
BROWNING
OF
WA
..........
73
5.3.
LOSS
OF
EP
AC
1
IN
ADIPOCYTES
..........................................................................................
74
5.3.1.
LOSS
OF
EPALI
IN
BA
AND
DOWNSTREAM
SIGNALING
...................................................
74
5.3.2.
EPALI
LOSS
DECREASES
WA
DIFFERENTIATION
AND
NE-MEDIATED
BROWNING
...............
75
5.3.3.
LOSS
OF
EPALI
IN
NEONATAL
BAT
AND
ADULT
ADIPOSE
TISSUE
.....................................
76
5.4.
EPAC1
LEVELS
IN
DIET-INDUCED
OBESITY
.............................................................................
76
6.
REFERENCES
..........................................................................................................................
78
SUMMARY
....................................................................................................................................
84
PUBLICATIONS
AND
ABSTRACTS
....................................................................................................
86
|
| any_adam_object | 1 |
| author | Reverte Salisa, Laia |
| author_GND | (DE-588)1177181657 |
| author_facet | Reverte Salisa, Laia |
| author_role | aut |
| author_sort | Reverte Salisa, Laia |
| author_variant | s l r sl slr |
| building | Verbundindex |
| bvnumber | BV046033689 |
| ctrlnum | (OCoLC)1108322277 (DE-599)DNB1179391020 |
| discipline | Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV046033689 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:33:21Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031415521 |
| oclc_num | 1108322277 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | V, 86 Seiten Illustrationen 21 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Reverte Salisa, Laia Verfasser (DE-588)1177181657 aut Distinct effects of cAMP effectors on brown adipocytes Laia Reverte Salisa Bonn 2019 V, 86 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinische Friedrich-Wilhelms-Universität Bonn 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1179391020/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031415521&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Reverte Salisa, Laia Distinct effects of cAMP effectors on brown adipocytes |
| subject_GND | (DE-588)4113937-9 |
| title | Distinct effects of cAMP effectors on brown adipocytes |
| title_auth | Distinct effects of cAMP effectors on brown adipocytes |
| title_exact_search | Distinct effects of cAMP effectors on brown adipocytes |
| title_full | Distinct effects of cAMP effectors on brown adipocytes Laia Reverte Salisa |
| title_fullStr | Distinct effects of cAMP effectors on brown adipocytes Laia Reverte Salisa |
| title_full_unstemmed | Distinct effects of cAMP effectors on brown adipocytes Laia Reverte Salisa |
| title_short | Distinct effects of cAMP effectors on brown adipocytes |
| title_sort | distinct effects of camp effectors on brown adipocytes |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1179391020/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031415521&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT revertesalisalaia distincteffectsofcampeffectorsonbrownadipocytes |
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