Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Aachen
Apprimus Verlag
2019
|
| Edition: | 1. Auflage |
| Series: | Applied Microbiology
Volume 12 |
| Subjects: | |
| Online Access: | Volltext Inhaltstext Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | XVII, 165 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 286 g |
| ISBN: | 9783863597160 3863597168 |
| DOI: | 10.18154/RWTH-2019-04428 |
Staff View
MARC
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| 245 | 1 | 0 | |a Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics |c Benedikt Wynands |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2019 | |
| 300 | |a XVII, 165 Seiten |b Illustrationen, Diagramme |c 21 cm x 14.8 cm, 286 g | ||
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| 650 | 0 | 7 | |a Aromaten |0 (DE-588)4143049-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas taiwanensis |0 (DE-588)1064240615 |2 gnd |9 rswk-swf |
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Record in the Search Index
| _version_ | 1804180076439273472 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
SUMMARY
........................................................................................................................................
V
ZUSAMMENFASSUNG
..............................................................................................................
VH
LIST
OF
ABBREVIATIONS
.........................................................................................................
IX
LIST
OF
FIGURES
.......................................................................................................................
XV
LIST
OF
TABLES
......................................................................................................................
XVH
1.
GENERAL
INTRODUCTION
.................................................................................................
3
1.1.
THE
REQUIRED
TRANSITION
INTO
A
SUSTAINABLE
BIO-BASED
ECONOMY
............................................
3
1.2.
SIGNIFICANCE
AND
PRODUCTION
OF
AROMATICS
............................................................................
3
1.3.
PSEUDOMONAS
AS
INDUSTRIAL
WORKHORSE
..................................................................................
5
1.4.
SOLVENT
TOLERANCE
MECHANISMS
OF
PSEUDOMONADS
................................................................
7
1.5.
BIPHASIC
FERMENTATION
SYSTEMS
.............................................................................................
10
1.6.
GENETIC
TOOLS
FOR
THE
ENGINEERING
OF
PSEUDOMONAS
............................................................
11
1.7.
METABOLIC
ENGINEERING
.........................................................................................................
13
1.8.
CHASSIS
ENGINEERING
...............................................................................................................
14
1.9.
MICROBIAL
PHENOL
PRODUCTION
...............................................................................................
15
1.10.
SCOPE
AND
OUTLINE
OF
THE
THESIS
.............................................................................................
19
2.
MATERIAL
AND
METHODS
..............................................................................................25
2.1.
MEDIA
AND
CULTURE
CONDITIONS
..............................................................................................
25
2.2.
PLASMID
CLONING,
STRAIN
ENGINEERING,
AND
GENOMIC
ANALYSIS
...............................................
26
2.3.
SWIM
ASSAY
............................................................................................................................32
2.4.
BIOFILM
FORMATION
ASSAY
.......................................................................................................
32
2.5.
SOLVENT
TOLERANCE
TESTS
..........................................................................................................
33
2.6.
ANALYTICAL
METHODS
..............................................................................................................
33
3.
RESULTS
..................................................................................................................................
37
3.1.
METABOLIC
ENGINEERING
OF
PSEUDOMONAS
TAIWANENSIS
VLB
120
WITH
MINIMAL
GENOMIC
MODIFICATIONS
FOR
HIGH-YIELD
PHENOL
PRODUCTION
................................................................
41
3.1.1.
ABSTRACT
.........................................................................................................................
41
3.1.2.
INTRODUCTION
..................................................................................................................
41
3.1.3.
INACTIVATION
OF
AROMATICS-DEGRADING
PATHWAYS
..........................................................
43
3.1.4.
EVALUATION
OF
DIFFERENT
TPL
GENES
FOR
OPTIMAL
PHENOL
PRODUCTION
..............................
46
3.1.5.
SIMULATION
OF
ENHANCED
SHIKIMATE
PATHWAY
FLUXES
THROUGH
QUINATE
CO-FEEDING
.... 48
3.1.6.
OPTIMIZATION
OF
PHENOL
PRODUCTION
FROM
GLUCOSE
THROUGH
REVERSE
ENGINEERING
..... 49
3.1.7.
OPTIMIZATION
OF
PHENOL
PRODUCTION
BY
COMBINATORIAL
FORWARD
ENGINEERING
............
52
3.1.8.
GROWTH
AND
PHENOL
PRODUCTION
OF
P.
TAIWANENSIS
VLB
1
20A5-TPL36
ON
GLUCOSE
AND
GLYCEROL
..................................................................................................................
55
3.1.9.
CONCLUSIONS
...................................................................................................................
57
3.2.
GENOME
REDUCTION
OF
PSEUDOMONAS
TAIWANENSIS
VLB
120
FOR
IMPROVED
BIOPROCESS
PERFORMANCE
...........................................................................................................................
61
3.2.1.
ABSTRACT
..........................................................................................................................
61
3.2.2.
INTRODUCTION
...................................................................................................................
61
3.2.3.
ELIMINATION
OF
SWIMMING
CAPABILITY
BY
THE
DELETION
OF
LARGE
FLAGELLAR
CLUSTERS
.....
63
3.2.4.
REDUCING
BIOFILM
FORMATION
BY
DELETION
OF
ADHESIVE
PROTEINS
..................................
66
3.2.5.
SOLVENT
TOLERANCE
AND
LOSS
OF
THE
PSTY
MEGAPLASMID
..............................................
68
3.2.6.
GROWTH
KINETICS
IN
MINIMAL
MEDIUM
WITH
GLUCOSE
.................................................... 70
3.2.7.
TTGGHI
TRANSPLANTATION
AND
DELETION
OF
PROVIRAL
DNA
SEGMENTS
.............................
73
3.2.8.
GENOME
REDUCTION
.........................................................................................................
74
3.2.9.
CONCLUSIONS
...................................................................................................................
77
3.3.
HIGH-YIELD
BIOPRODUCTION
OF
INDUSTRIALLY
RELEVANT
AROMATICS
WITH
STREAMLINED
CHASSIS
STRAINS
OF
PSEUDOMONAS
TAIWANENSIS
VLB
120
...................................................................
81
3.3.1.
ABSTRACT
.........................................................................................................................
81
3.3.2.
INTRODUCTION
...................................................................................................................
81
3.3.3.
FURTHER
METABOLIC
ENGINEERING
FOR
ENHANCED
PHENOL
PRODUCTION
..............................
82
3.3.4.
PHENOL
TOLERANCE
AND
THE
ASSOCIATED
IMPLICATION
OF
TTGGHI
.....................................
83
3.3.5.
METABOLIC
ENGINEERING
OF
THE
STREAMLINED
GRC
STRAINS
FOR
THE
OVERPRODUCTION
OF
HYDROXYLATED
AROMATICS
................................................................................................
85
3.3.6.
PRODUCTION
OF
PHENOL
FROM
GLYCEROL
USING
METABOLICALLY
ENGINEERED
GRC
1
............
86
3.3.7.
PRODUCTION
OF
4-VINYLPHENOL
........................................................................................
87
3.3.8.
ENZYME
SCREENING
FOR
EFFICIENT
AND
SPECIFIC
TYROSINE
AMMONIA-LYASE
ACTIVITY
......
89
3.3.9.
COMPARISON
OF
A
NON-STREAMLINED
PRODUCER
TO
GRC-BASED
PRODUCERS
.....................
91
3.3.10.
PRODUCTION
OF
4-VINYLPHENOL
FROM
GLYCEROL
USING
METABOLICALLY
ENGINEERED
GRC3
.............................................................................................................................
93
3.3.11.
CONCLUSIONS
...................................................................................................................
95
4.
GENERAL
DISCUSSION
AND
OUTLOOK
.......................................................................99
4.1.
THE
USE
OF
THE
GENERATED
PLATFORM
STRAINS
FOR
THE
PRODUCTION
OF
OTHER
AROMATICS
............
99
4.2.
ALTERNATIVE
FEEDSTOCKS
AND
VALORIZATION
OF
WASTE
STREAMS
...............................................
100
II
4.3.
STREAMLINED
CHASSIS
STRAINS
A
LA
CARTE
WITH
ENHANCED
PROCESS
PERFORMANCE
...................
103
4.4.
THE
CONCEPT
OF
*
METABOLIC
DEMAND
*
FOR
THE
DIRECTED
EVOLUTION
OF
ENHANCED
AROMATICS
PRODUCERS
AND
AN
INCREASED
PHENOTYPIC
STABILITY
...............................................................
104
4.5.
IMPROVEMENT
OF
PRODUCT
TITERS
AND
PRODUCTION
RATES
........................................................
107
4.6.
CONCLUSION
AND
IMPACT
OF
THIS
THESIS
..................................................................................
108
APPENDIX
.....................................................................................................................................
ILL
REFERENCES
...............................................................................................................................
141
CURRICULUM
VITAE
................................................................................................................
161
III
|
| any_adam_object | 1 |
| author | Wynands, Benedikt 1987- |
| author_GND | (DE-588)1185815619 |
| author_facet | Wynands, Benedikt 1987- |
| author_role | aut |
| author_sort | Wynands, Benedikt 1987- |
| author_variant | b w bw |
| building | Verbundindex |
| bvnumber | BV045907723 |
| collection | ebook |
| ctrlnum | (OCoLC)1104863670 (DE-599)DNB1186081376 |
| discipline | Allgemeine Naturwissenschaft |
| doi_str_mv | 10.18154/RWTH-2019-04428 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045907723 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:30:01Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783863597160 3863597168 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031290405 |
| oclc_num | 1104863670 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XVII, 165 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 286 g |
| psigel | ebook |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Wynands, Benedikt 1987- Verfasser (DE-588)1185815619 aut Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics Benedikt Wynands 1. Auflage Aachen Apprimus Verlag 2019 XVII, 165 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 286 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology Volume 12 Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2018 Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf Herstellung (DE-588)4159653-5 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Phenole (DE-588)4045721-7 gnd rswk-swf Reaktionstechnik (DE-588)4136173-8 gnd rswk-swf Aromaten (DE-588)4143049-9 gnd rswk-swf Pseudomonas taiwanensis (DE-588)1064240615 gnd rswk-swf Biokatalysator (DE-588)4006842-0 gnd rswk-swf Naturwissenschaften Biotechnologie angewandte Mikrobiologie (DE-588)4113937-9 Hochschulschrift gnd-content Pseudomonas taiwanensis (DE-588)1064240615 s Biotechnologie (DE-588)4069491-4 s Aromaten (DE-588)4143049-9 s DE-604 Phenole (DE-588)4045721-7 s Herstellung (DE-588)4159653-5 s Gentechnisch veränderter Organismus (DE-588)4353579-3 s Biokatalysator (DE-588)4006842-0 s Reaktionstechnik (DE-588)4136173-8 s Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2019-04428 Applied Microbiology Volume 12 (DE-604)BV042644516 12 https://doi.org/10.18154/RWTH-2019-04428 Resolving-System kostenfrei Volltext X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3d8443df2a154967bb77fb6957ee8183&prov=M&dok_var=1&dok_ext=htm Resolving-System kostenfrei Inhaltstext B:DE-101 application/pdf http://d-nb.info/1186081376/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031290405&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wynands, Benedikt 1987- Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics Applied Microbiology Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Herstellung (DE-588)4159653-5 gnd Biotechnologie (DE-588)4069491-4 gnd Phenole (DE-588)4045721-7 gnd Reaktionstechnik (DE-588)4136173-8 gnd Aromaten (DE-588)4143049-9 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Biokatalysator (DE-588)4006842-0 gnd |
| subject_GND | (DE-588)4353579-3 (DE-588)4159653-5 (DE-588)4069491-4 (DE-588)4045721-7 (DE-588)4136173-8 (DE-588)4143049-9 (DE-588)1064240615 (DE-588)4006842-0 (DE-588)4113937-9 |
| title | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics |
| title_auth | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics |
| title_exact_search | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics |
| title_full | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics Benedikt Wynands |
| title_fullStr | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics Benedikt Wynands |
| title_full_unstemmed | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics Benedikt Wynands |
| title_short | Engineering of Pseudomonas taiwanensis VLB120 for the sustainable production of hydroxylated aromatics |
| title_sort | engineering of pseudomonas taiwanensis vlb120 for the sustainable production of hydroxylated aromatics |
| topic | Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Herstellung (DE-588)4159653-5 gnd Biotechnologie (DE-588)4069491-4 gnd Phenole (DE-588)4045721-7 gnd Reaktionstechnik (DE-588)4136173-8 gnd Aromaten (DE-588)4143049-9 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Biokatalysator (DE-588)4006842-0 gnd |
| topic_facet | Gentechnisch veränderter Organismus Herstellung Biotechnologie Phenole Reaktionstechnik Aromaten Pseudomonas taiwanensis Biokatalysator Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2019-04428 http://deposit.dnb.de/cgi-bin/dokserv?id=3d8443df2a154967bb77fb6957ee8183&prov=M&dok_var=1&dok_ext=htm http://d-nb.info/1186081376/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031290405&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT wynandsbenedikt engineeringofpseudomonastaiwanensisvlb120forthesustainableproductionofhydroxylatedaromatics AT apprimusverlag engineeringofpseudomonastaiwanensisvlb120forthesustainableproductionofhydroxylatedaromatics |