Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells: = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Erlangen ; Nürnberg
2018
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IX, 118 Seiten Illustrationen, Diagramme |
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| 245 | 1 | 0 | |a Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells |b = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |c vorgelegt von Ljiljana Rokvić |
| 246 | 1 | 1 | |a Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| 264 | 1 | |a Erlangen ; Nürnberg |c 2018 | |
| 300 | |a IX, 118 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2018 | ||
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| 650 | 0 | 7 | |a Zelldifferenzierung |0 (DE-588)4067536-1 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Zelldifferenzierung |0 (DE-588)4067536-1 |D s |
| 689 | 0 | 2 | |a Hyperosmolarität |0 (DE-588)4301505-0 |D s |
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Record in the Search Index
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| adam_text | TABLE OF CONTENTS
FIGURES....................................................................................................................................................................I
TABLES.....................................................................................................................................................................II
ABBREVIATIONS......................................................................................................................................................ILL
ABSTRACT...............................................................................................................................................................VI
ZUSAMMENFASSUNG.....................................................................
......................................................................
VIII
1. INTRODUCTION........................................ X
1.1 IMMUNE
SYSTEM..........................................................................................................................................
1
1.1.1 B LYMPHOCYTE DEVELOPMENT AND M
ATURATION...................................................................................
2
1.1.2 B LYMPHOCYTE ACTIVATION AND
DIFFERENTIATION...................................................................................
4
1.1.3 TOLL-LIKE RECEPTOR
SIGNALING.................................................................................................................7
1.1.4 GENES INVOLVED IN THE B CELL TO PLASMA CELL TRA
NSITION.................................
..................................
9
1.1.5 SYNDECAN-1 (CD138) - PLASMA CELL M ARKER
.....................................................................................
11
1.2 OSMOLALITY AND OSMOTIC
STRESS..................................................................................
12
1.2.1 DIETARY SALT AND SALT
STORAGE...........................................................................................................
14
1.2.2 RELATIONSHIP BETWEEN NA+ AND THE IMMUNE
SYSTEM................................................. 15
2. SPECIFIC
AIMS..................................................................................................................................................
18
3.
RESULTS.............................................................................................................................................................21
3.1 EFFECT O F OSMOTIC STRESS ON DEVELOPMENT, SURVIVAL, ACTIVATION AND
IN VITRO
DIFFERENTIATION O F MOUSE
B
EEIL................................................................................................................................................................21
3.1.1 EXPRESSION PROFILES O F NFAT5 IN DIFFERENT STAGES O F B CELL M
ATURATION
.........................................
22
3.1.2 EFFECT O F INCREASED NACI CONCENTRATION ON SURVIVAL O F EARLY B
CELL PRECURSORS IN IL7 CULTURES... 23
3.1.3 EFFECT O F INCREASED NACI CONCENTRATIONS ON GROWTH AND VIABILITY O
F LIPOPOLYSACCHARIDE
ACTIVATED SPLENIC B
CELLS............................................................ ..
...............................
........... .................
25
3.1.4 EFFECT O F INCREASED NACI CONCENTRATIONS ON CELL CYCLE AND
PROLIFERATION O F LIPOPOLYSACCHARIDE
ACTIVATED SPLENIC B
CELLS.............................................................................................................................
27
3.1.5 EFFECT O F INCREASED NACI CONCENTRATIONS ON B CELL DIFFERENTIATION
INTO PLASMABLASTS
................
30
3.1.6 EFFECT O F INCREASED NACI CONCENTRATIONS ON ABUNDANCE O F SURFACE
IGM, IGH CLASS SWITCH
RECOMBINATION AND ANTIBODY PRODUCTION IN LPS BLASTS
...........................................................................
34
3.1.7 EFFECT OF INCREASED NACI CONCENTRATIONS ON NFAT5 EXPRESSION AND
P38/MAPK PHOSPHORYLATION
IN ACTIVATED MOUSE B
CELLS..........................................................................................................................
37
3.1.8 EFFECT OF VARIOUS OSMOTIC STIMULI ON B CELL GROWTH, VIABILITY AND
DIFFERENTIATION INTO LPS BLASTS
41
3.2
IN VIVO
EFFECTS OF DIETARY SALT ON B LYMPHOCYTE HOMEOSTASIS AND ANTIBODY
PRODUCTION
...............
45
3.2.1 ANALYSIS O F HOMEOSTASIS O F B CELL SUBSETS IN LYMPHATIC ORGANS O
F MICE RECEIVING A HIGH-SALT DIET
46
3.2.2 ANALYSIS O F GERMINAL CENTER B CELL FORMATION AND HOMEOSTASIS O F
PLASMA CELLS/PLASMABLASTS IN
MICE W ITH A HSD
INTAKE..............................................................................................................................
49
3.2.3 THE ANALYSIS O F ANTIBODY PRODUCTION ON A HIGH SALT D IE
T..........................................................57
3.3 CHARACTERIZATION O F SYNDECAN-1 (CD138) EXPRESSING B
CELLS........................................................... 60
3.3.1 CHARACTERIZATION O F CD138H,8H TACIINT/,OW SPLENIC CELLS
IN VIVO
.........................................................60
3.3.2 ANALYSIS O F CD138 EXPRESSION IN B CELL
CULTURES.............................................................................
63
3.3.3 ANALYSIS O F FETAL CALF SERUM INFLUENCE ON INDUCTION AND
MAINTENANCE O F CD138H,8H B CELLS
IN
VITRO
..............................................................................................................................................................
67
4.
DISCUSSION...................................................................................................................................................
69
4.1 EFFECT O F OSMOTIC STRESS ON DEVELOPMENT, SURVIVAL, ACTIVATION AND
IN VITRO
DIFFERENTIATION O F MOUSE
B
EEIL.................................................................................................................................................................
69
4.2
IN VIVO
EFFECTS O F DIETARY SALT ON B LYMPHOCYTE HOMEOSTASIS AND ANTIBODY
PRODUCTION
...................
79
4.3 CHARACTERIZATION O F SYNDECAN-1 (CD138) EXPRESSING B CELLS
...............................................................
84
5. FUTURE
PERSPECTIVES...................................................................................................................................
88
6. MATERIAL AND METHODS
90
6.1.1 MOUSE
STRAINS....................................................................................................................................90
6.1.2 MOUSE
DIETS...........................................................................................................................................
90
6.1.3 CELL CULTURE M
EDIA............................................................................................................................
91
6.1.4
CHEMICALS..........................................................................................................................................
91
6.1.5
SOLUTIONS............................................................................................................................................
91
6.1.6
BUFFERS...............................................................................................................................................
92
6.1.7
ANTIBODIES.........................................................................................................................................
93
6.1.8
OLIGONUCLEOTIDES..............................................................................................................................
95
6.1.9 MACHINES AND PLASTIC M
ATERIAL........................................................................................................
95
6.1.10 DATA BANKS AND
SOFTWARE...............................................................................................................96
6.2
METHODS..................................................................................................................................................96
6.2.1 SINGLE CELL ISOLATION FROM DIFFERENT
ORGANS.................................7
..................................................
96
6.2.2 ISOLATION O F NAIVE B CELLS BY NEGATIVE
SELECTION..............................................................................97
6.2.3 CELL
SORTING........................................................................................................................................97
6.2.4 CELL CU LTU RE
.....................................................................................
98
6.2.5 FLOW CYTOM
ETRY................................................................................................................................
98
6.2.6 ELISPOT
ANALYSIS.................................................................................................................................
99
6.2.7 RNA
ISOLATION.....................................................................................................................................99
6.2.8 REVERSE TRANSCRIPTION REACTION (CDNA
SYNTHESIS)........................................................................100
6.2.9 QUANTITATIVE REAL-TIME
PCR...........................................................................................................
100
7.
REFERENCES...................................................................................................................................................
101
8.
ACKNOWLEDGMENTS.......................................................................................................................................118
9. DECLARATION
119
|
| any_adam_object | 1 |
| author | Rokvić, Ljiljana |
| author_GND | (DE-588)1181362997 |
| author_facet | Rokvić, Ljiljana |
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| ctrlnum | (OCoLC)1121479826 (DE-599)DNB1176999540 |
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| language | English |
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| spelling | Rokvić, Ljiljana Verfasser (DE-588)1181362997 aut Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen vorgelegt von Ljiljana Rokvić Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen Erlangen ; Nürnberg 2018 IX, 118 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2018 Hyperosmolarität (DE-588)4301505-0 gnd rswk-swf Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf B-Lymphozyt (DE-588)4137121-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content B-Lymphozyt (DE-588)4137121-5 s Zelldifferenzierung (DE-588)4067536-1 s Hyperosmolarität (DE-588)4301505-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030833265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rokvić, Ljiljana Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen Hyperosmolarität (DE-588)4301505-0 gnd Zelldifferenzierung (DE-588)4067536-1 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| subject_GND | (DE-588)4301505-0 (DE-588)4067536-1 (DE-588)4137121-5 (DE-588)4113937-9 |
| title | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| title_alt | Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| title_auth | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| title_exact_search | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| title_full | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen vorgelegt von Ljiljana Rokvić |
| title_fullStr | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen vorgelegt von Ljiljana Rokvić |
| title_full_unstemmed | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen vorgelegt von Ljiljana Rokvić |
| title_short | Analysis of impact of hyperosmolality on development, activation and differentiation of B lymphoid cells |
| title_sort | analysis of impact of hyperosmolality on development activation and differentiation of b lymphoid cells analyse der auswirkungen von hyperosmolalitat auf die entwicklung aktivierung und differenzierung von b lymphoiden zellen |
| title_sub | = Analyse der Auswirkungen von Hyperosmolalität auf die Entwicklung, Aktivierung und Differenzierung von B-lymphoiden Zellen |
| topic | Hyperosmolarität (DE-588)4301505-0 gnd Zelldifferenzierung (DE-588)4067536-1 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| topic_facet | Hyperosmolarität Zelldifferenzierung B-Lymphozyt Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030833265&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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