Holdings: Role of lysine-specific histone demethylase 1a for kidney development and maintenance

Role of lysine-specific histone demethylase 1a for kidney development and maintenance:

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Bibliographic Details
Main Author:	Keller, Julia (Author)
Format:	Thesis Book
Language:	English
Published:	Freiburg im Breisgau 06/2018
Subjects:	Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	VI, 132 Seiten Illustrationen, Diagramme 30 cm

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MARC


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adam_text	CONTENT 1. INTRODUCTION ............................................................ 1.1 THE MAMMALIAN KIDNEY ................................... 1.1.1 GLOMERULAR FILTRA TIO N .............................. 1.1.2 TUBULAR REABSORPTION AND SECRETION ... 1.1.3 KIDNEY DEVELOPMENT ................... 1.2 EPIGENETICS ....................................................... 1.2.1 LYSINE-SPECIFIC HISTONE DEMETHYLASE L A . 1.2.2 EPIGENETICS AND KIDNEY ................ 1.3 AIM 2. MATERIAL AND METHODS............................................ 2.1 MATERIAL.......................................................... 2.1.1 ANTIBODIES............................................. 2.1.2 CHEMICALS .............................................. . 2.1.3 CONSUMABLES, KITS AND REAGENTS ......... . 2.1.4 EQUIPMENT............................................. 2.1.5 PRIMER.................................................... 2.1.6 SOFTWARE................................................ 2.1.7 SOLUTIONS AND BUFFERS ........................... 2.2 METHODS ......................................................... 2.2.1 MOUSE.................................................... 2.2.2 MOUSE STUDIES ...................................... 2.2.3 CRE-LOX SYSTEM ...................................... 2.2.4 MOUSE LINES........................................... 2.2.5 GENOTYPING............................................ 2.2.7 WESTERN BLOT ANALYSIS ........................... 2.2.8 IMMUNOFLUORESCENCE ............................ 2.2.9 HISTOLOGICAL ANALYSIS .............................. 2.2.10 TRANSMISSION ELECTRON MICROSCOPY ...... 2.2.11 URINE MEASUREMENT ............................. 2.2.12 UREA MEASUREMENT .............................. 2.2.13 NEPHRON COUNT ..................................... 2.2.14 PROGENITOR CELL ISOLATION ........................ 2.2.15 RNA ISOLATION AND SEQUENCING ANALYSIS 2.3 EXPERIMENTAL ANALYSIS AND STATISTICS ............ .5 .5 *6 ..8 11 20 23 26 29 30 30 30 30 31 33 34 34 35 40 40 40 41 42 43 50 52 53 53 54 55 56 56 57 59 3. RESULTS.................................................................................................................................................. 61 3.1 EXPRESSION ANALYSIS OF LSD1 IN WILDTYPE MICE............................................................................ 61 3.2 ABLATION OF LSD1 IN THE CAP MESENCHYME OF DEVELOPING KIDNEY ............................................. 62 3.3 CONDITIONAL DEPLETION OF LSD1 SLIGHTLY AFFECTS THE MURINE KIDNEY FUNCTION............................66 3.4 LSDL-DEPLETION LEADS TO CHANGES IN THE PROXIMAL TUBULAR SYSTEM .......................................... 68 3.5 DEMETHYLASE ACTIVITY OF LSD1 IS REQUIRED IN NEPHROGENESIS.....................................................70 3.6 ABLATION OF LSD1 IN THE TUBULAR SYSTEM LEADS TO MILD RENAL CHANGES ...................................... 72 3.7 LOSS OF LSD1 DURING NEPHRON FORMATION INDUCES MASSIVE CHANGES IN THE ADULT KIDNEY.........74 3.8 LSDL-DEFICIENCY IN THE CAP MESENCHYME RESULTS IN PREMATURE UPREGULATION O F NEPHRON- SPECIFIC GENES............................................................................................................................... 77 4. DISCUSSION............................................................................................................................................. 79 4.1 LSDL EXPRESSION IN WILDTYPE........................................................................................................ 79 4.2 EFFECTS OF LSDL-DEFICIENCY IN THE CAP MESENCHYME TO THE YOUNG KIDNEY...............................80 4.3 LSDL-DEPLETION IN THE TUBULAR SYSTEM ....................................................................................... 85 4.4 IMPACT OF CAP MESENCHYMAL LSDL-ABLATION ON THE ADULT KIDNEY ............................................ 87 4.5 LSD1 REPRESSES TRANSCRIPTIONAL ACTIVITY OF NEPHRON-SPECIFIC GENES ......................................... 90 5. SUMMARY..............................................................................................................................................94 5.1 ZUSAMMENFASSUNG.......................................................................................................................95 6. REFERENCES............................................................................................................................................96 7. ABBREVIATIONS.....................................................................................................................................118 7.1 GENERAL ABBREVIATIONS................................................................................................................118 7.2 MAGNITUE ORDER.......................................................................................................................... 123 7.3 UNITS............................................................................................................................................ 123 8. ADDITIONAL STUDIES............................................................................................................................. 124 9. STATUTORY DECLARATION / EIDESSTATTLICHE ERKLAERUNG.........................................................................125 10. ACKNOWLEDGEMENT / DANKSAGUNG................................................................................................... 126 11. SUPPLEMENT........................................................................................................................................128
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spelling	Keller, Julia Verfasser (DE-588)1169841090 aut Role of lysine-specific histone demethylase 1a for kidney development and maintenance vorgelegt von Julia Vornweg Freiburg im Breisgau 06/2018 VI, 132 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Albert-Ludwigs-Universität Freiburg im Breisgau 2017 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1170186319/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030804943&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Keller, Julia Role of lysine-specific histone demethylase 1a for kidney development and maintenance
subject_GND	(DE-588)4113937-9
title	Role of lysine-specific histone demethylase 1a for kidney development and maintenance
title_auth	Role of lysine-specific histone demethylase 1a for kidney development and maintenance
title_exact_search	Role of lysine-specific histone demethylase 1a for kidney development and maintenance
title_full	Role of lysine-specific histone demethylase 1a for kidney development and maintenance vorgelegt von Julia Vornweg
title_fullStr	Role of lysine-specific histone demethylase 1a for kidney development and maintenance vorgelegt von Julia Vornweg
title_full_unstemmed	Role of lysine-specific histone demethylase 1a for kidney development and maintenance vorgelegt von Julia Vornweg
title_short	Role of lysine-specific histone demethylase 1a for kidney development and maintenance
title_sort	role of lysine specific histone demethylase 1a for kidney development and maintenance
topic_facet	Hochschulschrift
url	http://d-nb.info/1170186319/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030804943&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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