Membrane protein purification and crystallization: a practical guide
Saved in:
| Format: | Electronic eBook |
|---|---|
| Language: | English |
| Published: |
Amsterdam ; Boston
Academic Press
2003
|
| Edition: | 2nd ed |
| Subjects: | |
| Item Description: | Print version record. - Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 |
| Physical Description: | 1 online resource (xviii, 316 pages) illustrations (some color) |
| ISBN: | 9780123617767 0123617766 9780080536170 0080536174 |
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| contents | This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins |
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| discipline | Biologie |
| edition | 2nd ed |
| format | Electronic eBook |
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| spelling | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger 2nd ed Amsterdam ; Boston Academic Press 2003 1 online resource (xviii, 316 pages) illustrations (some color) txt rdacontent c rdamedia cr rdacarrier Print version record. - Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Membrane proteins / Purification fast Proteinas larpcal Membranas (biologia) larpcal Bioquimica larpcal Membrane proteins Purification Kristallisation (DE-588)4033215-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf Reinigungsverfahren (DE-588)4391913-3 gnd rswk-swf Membranproteine (DE-588)4130026-9 s Reinigungsverfahren (DE-588)4391913-3 s 1\p DE-604 Kristallisation (DE-588)4033215-9 s 2\p DE-604 Jagow, Gebhard von Sonstige oth Schagger, Hermann Sonstige oth Erscheint auch als Druck-Ausgabe Membrane protein purification and crystallization 2nd ed Amsterdam ; Boston : Academic Press, 2003 0123617766 9780123617767 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Membrane protein purification and crystallization a practical guide This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Membrane proteins / Purification fast Proteinas larpcal Membranas (biologia) larpcal Bioquimica larpcal Membrane proteins Purification Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| subject_GND | (DE-588)4033215-9 (DE-588)4130026-9 (DE-588)4391913-3 |
| title | Membrane protein purification and crystallization a practical guide |
| title_auth | Membrane protein purification and crystallization a practical guide |
| title_exact_search | Membrane protein purification and crystallization a practical guide |
| title_full | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_fullStr | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_full_unstemmed | Membrane protein purification and crystallization a practical guide edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| title_short | Membrane protein purification and crystallization |
| title_sort | membrane protein purification and crystallization a practical guide |
| title_sub | a practical guide |
| topic | SCIENCE / Life Sciences / Anatomy & Physiology bisacsh SCIENCE / Life Sciences / Biochemistry bisacsh Membrane proteins / Purification fast Proteinas larpcal Membranas (biologia) larpcal Bioquimica larpcal Membrane proteins Purification Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd Reinigungsverfahren (DE-588)4391913-3 gnd |
| topic_facet | SCIENCE / Life Sciences / Anatomy & Physiology SCIENCE / Life Sciences / Biochemistry Membrane proteins / Purification Proteinas Membranas (biologia) Bioquimica Membrane proteins Purification Kristallisation Membranproteine Reinigungsverfahren |
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