Holdings: Genetic basis for natural variation in Cardamine hirsuta leaf shape

Genetic basis for natural variation in Cardamine hirsuta leaf shape:

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Bibliographic Details
Main Author:	Mane, Sébastien (Author)
Format:	Thesis Book
Language:	English
Published:	Köln 2017
Subjects:	Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	160 Seiten Illustrationen, Diagramme 30 cm

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MARC


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adam_text	TABLE OF CONTENTS CHAPTER 1: INTRODUCTION: LEAF DEVELOPMENTAL MECHANISMS, SHAPE DIVERSITY AND ADAPTIVE SIGNIFICANCE................................................................................................................. 1 1. MOLECULAR PATHWAYS UNDERLYING LEAF DEVELOPEMENT AND ITS DIVERSITY ..................... 2 1.1. INITIATION AND DEVELOPMENTAL CONTROL OF LEAF GROW TH........................................2 1 .1 .1 . LEAF INITIATION FROM THE FLANKS O F THE SHOOT APICAL MERISTEM ................... 2 1. 1.2. AXIAL POLARITY, PATTERNING AND LEAF MORPHOGENESIS ................................... 4 1. 2. MODIFICATION IN THE DEVELOPMENTAL TIMING GENERATES SHAPE DIVERSITY WITHIN SPECIES.............................................................................................................................8 1. 3. USING INTRASPECIFIC NATURAL VARIATION IN COMPARATIVE DEVELOPMENT STUDIES ..11 2. THE EVOLUTION AND FUNCTIONAL SIGNIFICANCE O F LEAF SHAPE DIVERSITY ........................ 12 2. 1. DEFINITION, ORIGIN AND DIVERSITY O F LEAVES ....................................................... 12 2.2. CAUSES AND CONSEQUENCES O F LEAF SHAPE DIVERSITY...........................................14 2 .2 .1 . EVOLUTIONARY AND DEVELOPMENTAL FORCES INFLUENCING LEAF SHAPE ............. 14 2. 2.2. FUNCTIONAL SIGNIFICANCE O F LEAF SHAPE VARIATION AND ITS POTENTIAL ECOLOGICAL DRIVERS..................................................................................................... 16 3. THESIS AIMS AND OBJECTIVES........................................................................................ 18 CHAPTER 2: VARIATION IN LEAF MORPHOLOGY WITHIN CARDAMINE HIRSUTA SPECIES................. 20 INTRODUCTION...........................................................................................................................21 RESULTS . ................................................................................................................................... 22 1. LEAF MORPHOLOGY CHARACTERISTICS..............................................................................22 2. CHARACTERISTICS OF THE C. HIRSUTA AZORES STRAIN (A Z#L)...........................................23 DISCUSSION..............................................................................................................................28 CHAPTER 3: FINE MAPPING OF A QTL UNDERLYING LEAFLET NUMBER VARIATION IN C HIRSU.TA.29 INTRODUCTION...........................................................................................................................30 RESULTS.................................................................................................................................... 31 1. QTL MAPPING IN THE FE OXFORD/AZORES RIL POPULATION........................................ 31 1.1. QTL ASSOCIATED WITH THE LEAFLET NUMBER TRAITS AND ROSETTE LEAF NUM BER.........32 1. 2. QTL ASSOCIATED WITH THE LEAF FIVE AREA, LENGTH AND SHAPE.............................37 1.3. CHARACTERIZATION OF A LOCUS UNDERLYING LOW LEAFLET NUMBER VARIATION............39 1 .3 .1 . QTL VALIDATION AND FINE MAPPING WITH HETEROZYGOUS INBRED FAMILIES 39 1. 3. 2. LOCUS VALIDATION WITH NEAR ISOGENIC LINE............................................... 44 2. CLONING, GENETIC COMPLEMENTATION AND IDENTIFICATION OF THE QUANTITATIVE TRAIT NUCLEOTIDE......................................................................................................................... 46 2.1. CANDIDATE GENE SELECTION IN THE QTL-LG4_@ 106.........................................46 2 .1 .1 . SPL9 IS A GOOD CANDIDATE GENE.................................................................49 2 .1 .2 . SPL9 PHYTOGENY AND ALLELE SPECIFIC TO THE AZORES ISLANDS ..................... 51 2.2. EVIDENCE FOR SPL9 UNDERLYING QTL-LG4_@ 106 BY GENETIC COMPLEMENTATION.......................................................................................................... 53 2.2. 1. TRANSGENIC TEST OF THE GENOMIC SPL9 ALLELES ......................................... 53 2. 2. 2. TRANSGENIC TEST OF THE SPL9 CODING SEQUENCES ........................................ 57 2. 2. 1. TRANSGENIC TEST WITH OVEREXPRESSION OF SPL9 ALLELES ............................. 58 2. 1. EVIDENCE FOR THE QTN IN SPL9 UNDERLYING QTL-LG4_@ 106.......................60 3. ESTIMATION OF THE SPL9 ALLELIC GENETIC EFFECT..........................................................62 4. FUNCTIONAL COMPLEMENTATION OF THE SPL9 MUTANT WITH OX AND AZ#L SPL9 ALLELES 64 4.1. C. HIRSUTA SPL9 KNOCKOUT DECREASES LEAFLET NUM BER......................................64 4. 2. FUNCTIONAL COMPLEMENTATION OF THE SPL9 MUTANT WITH SPL9 OXFORD AND AZORES ALLELES............................................................................................................... 66 DISCUSSION.............................................................................................................................. 69 C HAPTER 4: C HARACTERIZATION OF SPL9 ALLELES AND THEIR MOLECULAR, PHYSIOLOGICAL AND PHENOTYPIC EFFECTS IN C. HIRSUTA...............................................................................................70 INTRODUCTION........................................................................................................................... 71 RESULTS.................................................................................................................................... 72 1. SPL9 ALLELIC EFFECTS ON PLANT DEVELOPMENT............................................................... 72 1.1. SPL9 ALLELE EFFECT ON THE JUVENILE TO ADULT PHASE TRANSITION ........................... 72 1. 2. SPL9 ALLELIC EFFECT ON THE TIMING OF LEAF EMERGENCE.....................................74 1.3. SPL9 ALLELIC EFFECT ON THE LEAF GROWTH............................................................75 1. 4. ANALYSIS OF THE HETEROBLASTIC LEAF SHAPE PROGRESSION.....................................77 1. 4.1. THE INFLUENCE OF SPL9 ALLELIC VARIATIONS ON LEAVES LENGTHS, AREAS AND PERIMETERS................................................................................................................ 77 1. 4.2. SPL9 EFFECTS ON TERMINAL LEAFLET SHAPE.....................................................82 1. 5. SPL9 ALLELIC INFLUENCE ON THE AUXIN PATTERNS IN TERMINAL LEAFLETS ................. 85 1. 6. SPL9 ALLELIC EFFECT ON THE CELL NUMBER AND CELL SIZE.....................................86 2. CHARACTERIZATION OF FITNESS-RELATED TRAITS INFLUENCED BY SPL9 ALLELES .................... 89 2. 1. YIELD PARAMETERS INFLUENCED BY THE SPL9 ALLELE STATE.....................................89 2.2. REPRODUCTIVE PLASTICITY MEASURES .................................................................... 91 3. MOLECULAR FEATURES OF THE TWO SPL9 PROTEIN VARIANTS ............................................. 97 3.1. ESTIMATION OF THE DNA-PROTEIN BINDING CAPACITIES ........................................ 97 3.2. SPL9 INTERACTION WITH THE DELLAS-GA SIGNALLING PATHWAY ........................ 99 DISCUSSION............................................................................................................................ 106 C HAPTER 5: G ENERAL DISCUSSION AND PERSPECTIVES .............................................................. I L L 1. REGULATION OF PHASE CHANGE, A KEY EVOLUTIONARY MECHANISM TO GENERATE LEAF SHAPE DIVERSITY............................................................................................................................ 112 2. ECOLOGICAL SIGNIFICANCE OF LEAVES MORPHOLOGICAL VARIATION .................................. 113 CHAPTER 6: MATERIALS AND METHODS...................................................................................... 115 1. GROWING CONDITIONS AND PLANT MATERIALS.................................................................116 1.1. GROWING CONDITIONS ......................................................................................... 116 1.2. STRAINS USED AND LINES CREATED.........................................................................116 2. QTL ANALYSIS............................................................................................................ 117 3. TRANSGENIC PLANTS GENERATION.................................................................................. 118 3.1. CONSTRUCTS AND CLONING STRATEGIES .................................................................. 118 3. 2. GENETIC COMPLEMENTATION.............................................................................. 120 3.3. CRISPR-CAS9 MEDIATED MUTAGENESIS............................................................ 120 4. PHENOTYPIC AND PHYSIOLOGIC ANALYSIS......................................................................120 4.1. LEAF MORPHOMETRIC ANALYSIS........................................................................... 120 4.2. ANALYSIS OF SEED PHENOTYPE........................................................................... 121 4.3. PHOTOPERIOD-SHIFT EXPERIMENT.........................................................................121 4. 4. CONFOCAL MICROSCOPY......................................................................................121 5. MOLECULAR BIOLOGY METHODS.................................................................................... 122 5.1. PRIMER SEQUENCES............................................................................................122 5. 2. DNA EXTRACTION AND GENOTYPING....................................................................124 5. 3. RNA EXTRACTION AND QRT-PCR......................................................................124 5.4. YEAST TWO-HYBRID (Y2H) ................................................................................ 124 5. 5. ELECTROPHORETIC MOBILITY SHIFT ASSAY (EM SA)............................................124 BIBLIOGRAPHY........................................................................................................................126 APPENDIX.......................................................................................................................... 135 ACKNOWLEDGEMENTS........................................................................................................ 158 LIST OF ABBREVIATIONS 159
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author	Mane, Sébastien
author_GND	(DE-588)1151961256
author_facet	Mane, Sébastien
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discipline	Biologie
format	Thesis Book
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spelling	Mane, Sébastien Verfasser (DE-588)1151961256 aut Genetic basis for natural variation in Cardamine hirsuta leaf shape vorgelegt von Sébastien Mane Köln 2017 160 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1155026276/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030663357&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Mane, Sébastien Genetic basis for natural variation in Cardamine hirsuta leaf shape
subject_GND	(DE-588)4113937-9
title	Genetic basis for natural variation in Cardamine hirsuta leaf shape
title_auth	Genetic basis for natural variation in Cardamine hirsuta leaf shape
title_exact_search	Genetic basis for natural variation in Cardamine hirsuta leaf shape
title_full	Genetic basis for natural variation in Cardamine hirsuta leaf shape vorgelegt von Sébastien Mane
title_fullStr	Genetic basis for natural variation in Cardamine hirsuta leaf shape vorgelegt von Sébastien Mane
title_full_unstemmed	Genetic basis for natural variation in Cardamine hirsuta leaf shape vorgelegt von Sébastien Mane
title_short	Genetic basis for natural variation in Cardamine hirsuta leaf shape
title_sort	genetic basis for natural variation in cardamine hirsuta leaf shape
topic_facet	Hochschulschrift
url	http://d-nb.info/1155026276/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030663357&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT manesebastien geneticbasisfornaturalvariationincardaminehirsutaleafshape

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