Microfluidics in single cells:
Saved in:
| Other Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Edition: | First edition |
| Series: | Methods in cell biology
Volume 147 Microfluidics in cell biology Part B |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xv, 231 Seiten Illustrationen |
| ISBN: | 9780128142820 |
Staff View
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Record in the Search Index
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| adam_text | Contents
Contributors...........................................................ix
Preface................................................................xv
SECTION 1 MICROFLUIDICS FOR MICRO-ORGANISMS
CHAPTER 1 Drug delivery and temperature control in
microfluidic chips during live-cell imaging
experiments.............................................................3
Javier Munoz-Garcia, Julien Babic, Damien Coudreuse
1, Introduction..............................................4
2« Fabrication of the Microfluidic Chip......................7
3. Setting Up and Using the Microfluidic System............18
4. Drug Delivery in the Microsystem........................23
5. General Conclusions: Pros and Cons......................25
Acknowledgments.............................................27
References..................................................27
CHAPTER 2 Controllable stress patterns over multi-generation
timescale in microfluidic devices........................29
Youlian Goulev, Audrey Matifas, Gilles Charvin
1. Introduction............................................30
2. Method................................................ 32
3. Microfluidic Chip Design................................32
4. PDMS Chip Fabrication...................................32
5. Microfluidics Live-Cell Imaging Setup...................35
6. Principles of H202 Ramping Stress.......................36
7. Stress Ramping Setup....................................38
8. Discussion..............................................39
References..................................................39
CHAPTER 3 A bacterial antibiotic resistance accelerator
and applications.........................................41
Julia Bos, Robert H. Austin
1. Introduction.............................................42
2. Materials...............................................43
3. Methods.................................................44
4. Device Performances.....................................50
5. Conclusions.............................................54
References..................................................55
Further Reading.............................................57
VI
Contents
CHAPTER 4 A microfluidic fluidized bed to capture,
amplify and detect bacteria from raw
samples...............................................59
Lucile Alexandre, lago Pereiro, Amel Bendali, Sanae Tabnaoui,
Jana Srbova, Zuzana Bilkova, Shane Deegan, Lokesh Joshi,
Jean-Louis Viovy, Laurent Malaquin, Bruno Dupuy,
Stéphanie Descroix
1. Introduction...........................................60
2. Method.................................................61
3. Discussion.............................................73
References...............................................75
SECTION 2 MICROFLUIDICS FOR CELL MIGRATION
CHPATER 5 Micro-engineered “pillar forests” to study cell
migration in complex but controlled 3D
environments...........................................79
Jörg Renkawitz, Anne Reversât, Alex Leithner, Jack Merrin,
Michael Sixt
1. Introduction.......................................... 80
2. Method.................................................82
3. Pitfalls and Limitations...............................87
4. Discussion.............................................87
References................................................90
CHAPTER 6 Measuring spontaneous neutrophil motility
signatures from a drop of blood using
microfluidics..........................................93
Sinan Muldur, Anika L. Marand, Felix Ellett,
Daniel Irimia
1. Introduction...........................................94
2. Microfluidic Device....................................95
3. Sample Preparation and Loading.........................98
4. Microscopy............................................100
5. Data Analysis.........................................102
6. Conclusion............................................106
Acknowledgments..........................................106
References...............................................106
Contents
VII
CHAPTER 7 Directing cell migration on flat substrates
and in confinement with microfabrication
and microfluidics.......................................109
Emilie Le Maout, Simon Lo Vecchio, Alka Bhat,
Daniel Riveline
1. Introduction............................................110
2. Microfabrication of Motifs..............................114
3. Rectifying Cell Motion With Asymmetrical Patterns:
Micro-Contact Printing of Adhesive Motifs...............118
4. Control of Cell Migration in Confined 3D Environment....121
5. Control of Cell Migration in 3D With Chemotaxis.........126
6. Troubleshooting.........................................130
7. Conclusion..............................................131
References.................................................132
CHAPTER 8 3D cell migration in the presence of chemical
gradients using microfluidics...........................133
Andrew G. Clark, Anthony Simon, Koceila Aizel,
Jérôme Bibette, Nicolas Bremond, Danijela Marie Vignjevic
1. Introduction............................................134
2. Gradient device fabrication.............................135
3. Chamber preparation for live imaging experiments........138
4. Data analysis...........................................143
5. Common Experimental Problems and Troubleshooting........144
6. Conclusion..............................................145
Appendix. Example Collagen Recipe..........................146
References.................................................146
SECTION 3 MICROFLUIDICS FOR CELL MECHANICS
CHAPTER 9 Microfluidics for cell sorting and single cell
analysis from whole blood.................................m
Ramanathan Vaidyanathan, Trifanny Yeo, Chwee Teck Lim
1. Introduction............................................152
2. Cell Sorting and CTC Isolation Using Spiral
Microfluidic Chip.......................................155
3. Isolation and Retrieval of Single CTCs..................164
4. Conclusion..............................................170
References.................................................171
Contents
CHAPTER 10 High-throughput single-cell mechanical
phenotyping with real-time deformability
cytometry...............................................ns
Marta Urbanska, Philipp Rosendahl, Martin Kráter,
Jochen Guck
1. Introduction...........................................176
2. Method Overview........................................177
3. Experimental Protocols.................................180
4. Data Analysis..........................................188
5. Discussion.............................................193
Acknowledgments...........................................196
References................................................196
CHAPTER 11 A microfluidic cell-trapping device to study
dynamic host-microbe interactions at the
single-cell level......................................199
Chiara Toniolo, Matthieu Delineé, John D. McKinney
1. Introduction...........................................200
2. Protocol...............................................201
3. Discussion.............................................211
References................................................213
CHAPTER 12 Mechano-chemostats to study the effects
of compressive stress on yeast.............215
L.J. Holt, 0. Hallatschek, M. Delarue
1. Introduction...........................................216
2. Presentation and Microfabrication of the
Mechano-Chemostat......................................218
3. Control of the Mechanical Environment..............223
4. Physiological Response to a Mechanical Stress..........227
5. Discussion.............................................228
References................................................229
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| series | Methods in cell biology Microfluidics in cell biology |
| series2 | Methods in cell biology Microfluidics in cell biology |
| spelling | Microfluidics in single cells edited by Matthieu Piel, Daniel Fletcher, Junsang Doh First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xv, 231 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 147 Microfluidics in cell biology Part B Mikrofluidik (DE-588)4803438-1 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrofluidik (DE-588)4803438-1 s Cytologie (DE-588)4070177-3 s DE-604 Piel, Matthieu 1973- (DE-588)116571213X edt Fletcher, Daniel (DE-588)116571180X edt Doh, Junsang (DE-588)1165711648 edt Methods in cell biology Volume 147 (DE-604)BV002534878 147 Microfluidics in cell biology Part B (DE-604)BV045262771 B Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030650706&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Microfluidics in single cells Methods in cell biology Microfluidics in cell biology Mikrofluidik (DE-588)4803438-1 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4803438-1 (DE-588)4070177-3 (DE-588)4143413-4 |
| title | Microfluidics in single cells |
| title_auth | Microfluidics in single cells |
| title_exact_search | Microfluidics in single cells |
| title_full | Microfluidics in single cells edited by Matthieu Piel, Daniel Fletcher, Junsang Doh |
| title_fullStr | Microfluidics in single cells edited by Matthieu Piel, Daniel Fletcher, Junsang Doh |
| title_full_unstemmed | Microfluidics in single cells edited by Matthieu Piel, Daniel Fletcher, Junsang Doh |
| title_short | Microfluidics in single cells |
| title_sort | microfluidics in single cells |
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| topic_facet | Mikrofluidik Cytologie Aufsatzsammlung |
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