The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis: = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Erlangen-Nürnberg
[2018]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 112 Seiten Illustrationen, Diagramme |
Internformat
MARC
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| 007 | t | ||
| 008 | 181015s2018 gw a||| m||| 00||| eng d | ||
| 016 | 7 | |a 1168836301 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1090118154 | ||
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| 100 | 1 | |a Wohlfahrt, Thomas Georg |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis |b = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |c vorgelegt von Thomas Georg Wohlfahrt |
| 246 | 1 | 1 | |a <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| 264 | 1 | |a Erlangen-Nürnberg |c [2018] | |
| 300 | |a 112 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2018 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030621234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030621234 | ||
Datensatz im Suchindex
| _version_ | 1804178961373069312 |
|---|---|
| adam_text | 1. INTRODUCTION
1
1.1.
FIBROBLASTS...............................................................................................................1
1.1.1.
ORIGIN.......................................................................................................................1
1.1.2.
PLASTICITY.................................................................................................................
2
1.1.3.
FUNCTION..................................................................................................................
3
1.1.4. PHYSIOLOGICAL VS. PATHOLOGICAL
FIBROBLASTS...............................................................5
1.1.5. FIBROTIC
FIBROBLASTS..................................................................................................
5
1.1.6. INFLAMMATORY MATRIX-DEGRADING
FIBROBLASTS............................................................9
1.2. FIBROTIC
DISEASES....................................................................................................
11
1.2.1. SYSTEMIC
SCLEROSIS..................................................................................................11
1.2.1.1.
VASCULOPATHY.........................................................................................................
12
1.2.1.2. CELLULAR IMMUNE ACTIVATION AND
AUTOIMMUNITY......................................................13
1.2.1.3.
FIBROSIS..................................................................................................................
14
1.2.2. OTHER FIBROTIC
DISEASES...........................................................................................
14
1.3. CHRONIC INFLAMMATORY DISEASES (RHEUMATOID ARTHRITIS)
.........................................
15
1.4. THE ETS FAMILY OF TRANSCRIPTION FACTORS
...............................................................
16
1.5. AIM OF THE
STUDY....................................................................................................
18
2. MATERIAL AND
METHODS......................................................................................................19
2.1.
MATERIALS................................................................................................................19
2.1.1.
CHEMICALS..............................................................................................................19
2.1.2.
INSTRUMENTS...........................................................................................................
23
2.1.3. COMMERCIALLY AVAILABLE SYSTEMS/KITS
...................................................................
25
2.1.4. AUXILIARY
MATERIALS................................................................................................26
2.1.5. ANTIBODIES AND DYES USED FOR IMMUNOFLUORESCENCE
IMAGING................................28
2.1.6. DYES USED IN FLOW CYTOMETRY
................................................................................
30
2.1.7. ANTIBODIES USED FOR WESTEM BLOT ANALYSIS
2.1.8. ANTIBODIES USED FOR CHROMATIN IMMUNOPRECIPITATION (CHIP) ANALYSIS
..................
32
2.1.9. PRIMER USED FOR GENE EXPRESSION
ANALYSIS...............................................................
32
2.2. MEDIA, BUFFERS AND
SOLUTIONS..................................................................................35
2.2.1. FIBROBLAST GROWTH
MEDIA.........................................................................................35
2.2.2. KERATINOCYTE GROWTH MEDIA
..................................................................................
35
2.2.3. BUFFERS FOR FLOW CYTOMETRY
...................................................................................
36
2.2.4. HEMATOXYLIN/EOSIN STAINING
.................................................................................
36
2.2.5. HYDROXYPROLINE ASSAY
...........................................................................................
37
2.2.6. IMMUNOHISTOCHEMISTRY
.........................................................................................
37
2.2.7. SDS-PAGE AND WESTERN BLOT
..............................................................................
38
2.3.
METHODS..................................................................................................................39
2.3.1. EXPERIMENTAL APPROACHES
......................................................................................
39
2.3.2. PATIENT
STUDIES.........................................................................................................39
2.3.3. CELL
CULTURE...........................................................................................................
40
2.3.3.1. HUMAN FIBROBLAST CULTURE
.......................................................................................
40
2.3.3.2.
NUCLEOFECTION........................................................................................................
40
2.3.3.3. CELL VIABILITY AND CYTOTOXICITY ASSAYS
...................................................................
41
2.3.3.4. PREPARATION OF MICROMASS
CULTURES.........................................................................41
2.3.3.5. GENERATION OF ORGANOTYPIC SKIN
CULTURES.................................................................41
2.3.3.6. MURINE CELL
ISOLATION..............................................................................................
42
2.3.4. GENE-EXPRESSION
ANALYSIS.....................................................................................
42
2.3.4.1. RNA
ANALYSIS.........................................................................................................
42
2.3.4.2. REPORTER
ASSAYS......................................................................................................
43
2.3.43. CHIP
ASSAYS............................................................................................................
43
2.3.4.4. BISULFITE
PYROSEQUENCING.......................................................................................
43
23.4.5. RNA
SEQUENCING...................................................................................................44
23.4.6. IN-SILICO ANALYSIS FOR POSSIBLE MICRORNA BINDING SITES FOR PU.
1.........................44
2.3.5. PROTEIN
ANALYSIS.....................................................................................................
44
2.3.5.1. WESTERN BLOT
ANALYSIS.............................................................................................44
2.3.5.2. QUANTIFICATION OF COLLAGEN
PROTEIN.........................................................................45
2.3.5.3. IMMUNOHISTOCHEMISTRY AND IMMUNOFLUORESCENCE STAINING
...................................
45
2.3.5.4. FLOW
CYTOMETRY......................................................................................................45
2.3.6. HISTOLOGICAL
ANALYSIS............................................................................................
46
2.3.6.1. HEMATOXYLIN/EOSIN
STAINING..................................................................................46
2.3.6.2. MASSON*S TRICHROME STAINING
.................................................................................
46
2.3.6.3. SIRIUS RED
STAINING.................................................................................................
46
2.3.7. ANIMAL
STUDIES......................................................................................................
47
2.3.7.1. KNOCKOUT OF PU
.L..................................................................................................47
2.3.7.2. PROFIBROTIC MOUSE
MODELS......................................................................................47
2.3.7.3. MOUSE MODEL OF PHYSIOLOGICAL WOUND
HEALING...................................................... 48
2.3.8. STATISTICAL
ANALYSIS................................................................................................
49
3.
RESULTS................................................................................................................................
50
3.1. UPREGULATION OF PU. 1 IN FIBROBLASTS UNDER FIBROTIC
CONDITIONS..............................50
3.2. FIBROGENIC POTENTIAL OF PU. 1 -EXPRESSING FIBROBLASTS
.............................................
57
3.3. UPSTREAM REGULATION OF PU.L IN RESTING, FIBROTIC AND INFLAMMATORY
FIBROBLASTS.... 65
3.4. PU.L -DEPENDENT FIBROTIC TISSUE
REMODELING...........................................................71
3.5. PU. 1 -SILENCING IN EXPERIMENTAL FIBROSIS AND WOUND HEALING MODELS
....................
79
4.
DISCUSSION..........................................................................................................................
87
4.1. PU. 1 EXPRESSION IS CONTROLLED BY EPIGENETIC AND
POSTTRANSCRIPTIONAL MECHANISMS87
4.2. PU. 1
EXPRESSION CONTROLS FIBROBLAST POLARIZATION AND TISSUE FIBROSIS
.....................
89
4.3. PU. 1 INHIBITION AS POTENTIAL DRUG THERAPY FOR CLINICAL USE
.....................................
90
5. CONCLUDING
REMARKS.........................................................................................................
92
6.
APPENDICES........................................................................................................................
93
7. REFERENCES 98
|
| any_adam_object | 1 |
| author | Wohlfahrt, Thomas Georg |
| author_facet | Wohlfahrt, Thomas Georg |
| author_role | aut |
| author_sort | Wohlfahrt, Thomas Georg |
| author_variant | t g w tg tgw |
| building | Verbundindex |
| bvnumber | BV045232885 |
| ctrlnum | (OCoLC)1090118154 (DE-599)DNB1168836301 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045232885 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:12:17Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030621234 |
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| owner_facet | DE-29T DE-29 DE-355 DE-BY-UBR |
| physical | 112 Seiten Illustrationen, Diagramme |
| publishDate | 2018 |
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| spelling | Wohlfahrt, Thomas Georg Verfasser aut The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose vorgelegt von Thomas Georg Wohlfahrt <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose Erlangen-Nürnberg [2018] 112 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2018 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030621234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wohlfahrt, Thomas Georg The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| subject_GND | (DE-588)4113937-9 |
| title | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| title_alt | <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| title_auth | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| title_exact_search | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| title_full | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose vorgelegt von Thomas Georg Wohlfahrt |
| title_fullStr | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose vorgelegt von Thomas Georg Wohlfahrt |
| title_full_unstemmed | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose vorgelegt von Thomas Georg Wohlfahrt |
| title_short | The ETS family transcription factor PU.1 controls fibroblast polarization and tissue fibrosis |
| title_sort | the ets family transcription factor pu 1 controls fibroblast polarization and tissue fibrosis der ets transkriptionsfaktor pu 1 kontrolliert fibroblastenpolarisation und organfibrose |
| title_sub | = <Der ETS-Transkriptionsfaktor PU.1 kontrolliert Fibroblastenpolarisation und Organfibrose |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030621234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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