Holdings: Regulation of adipose tissue homeostasis by the death ligand TRAIL

Regulation of adipose tissue homeostasis by the death ligand TRAIL:

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Bibliographic Details
Main Author:	Zoller, Verena 1987- (Author)
Format:	Thesis Book
Language:	English
Published:	Ulm [2018]
Subjects:	Fettsucht Obesity. TNF-related apoptosis-inducing ligand. Hochschulschrift
Online Access:	Inhaltsverzeichnis Inhaltsverzeichnis
Physical Description:	101 Seiten Illustrationen, Diagramme 30 cm

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MARC


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adam_text	TABLE OF CONTENTS LIST OF ABBREVIATIONS 6 1. INTRODUCTION 9 1.1 OBESITY 9 1.2 OBESITY AND ADIPOSE TISSUE 9 1.3 FUNCTION OF THE WAT 10 1.4 REGULATION OF ADIPOSE TISSUE MASS 12 1.4.1 PROLIFERATION 13 1.4.2 ADIPOGENIC DIFFERENTIATION 14 1.5 INFLAMMATION OF THE ADIPOSE TISSUE 15 1.6 APOPTOSIS IN ADIPOSE TISSUE 17 1.7 TRAIL 18 1.8 NON-APOPTOTIC FUNCTIONS OF DEATH LIGANDS IN ADIPOSE TISSUE 20 2. AIM OF THE STUDY 22 3. MATERIAL AND METHODS 23 3.1 MATERIALS 23 3.1.1 DEVICES 23 3.1.2 REAGENTS 23 3.1.3 OLIGONUCLEOTIDES 24 3.1.4 ANTIBODIES 26 3.1.5 ELISAS 27 3.1.6 ENZYMES 27 3.1.7 VECTORS 27 3.1.8 CELL CULTURE 27 3.2 METHODS 29 3.2.1 CELL CULTURE 29 3.2.2 DETERMINATION OF CELL NUMBER 30 3.2.3 PRIMARY HUMAN ADIPOSE STROMAL CELL ISOLATION 30 3.2.4 RNA ISOLATION AND CDNA PREPARATION 31 3.2.5 MRNA EXPRESSION ARRAYS 31 3.2.6 QUANTITATIVE REAL-TIME PCR (QPCR) 32 3.2.7 PROTEIN EXTRACTION 33 3.2.8 BRADFORD ASSAY 33 3.2.9 WESTERN BLOT 34 3.2.10 TRIGLYCERIDE ASSAY 34 3.2.11 LENTIVIRAL MEDIATED KNOCKDOWN OF CASPASE-8 35 3.2.12 CASPASE ACTIVITY ASSAY 35 3.2.13 NICOLETTI ASSAY 35 3.2.14 ELISA 36 3.2.15 ELECTROPHORETIC MOBILITY SHIFT ASSAY (EMSA) 36 3.2.16 REPORTER GENE ASSAY 38 3.2.17 STATISTICAL ANALYSIS 38 4. RESULTS 39 4.1 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION 39 4.1.1 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION IN HUMAN SGBS CELLS 39 4.1.2 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION IN HUMAN PRIMARY CELLS 40 4.1.3 THE INHIBITORY EFFECT OF TRAIL IS MEDIATED VIA TRAIL-R2 41 4.1.4 THE ANTI-ADIPOGENIC EFFECT OF TRAIL IS NOT MEDIATED VIA THE NON-CANONICAL TRAIL SIGNALING 42 4.1.4 CASPASES ARE RESPONSIBLE FOR THE INHIBITORY EFFECT OF TRAIL ON ADIPOGENESIS 44 4.1.5 TRAIL BLOCKS THE UPREGULATION OF ADIPOGENIC TRANSCRIPTION FACTORS 47 4.2 TRAIL INDUCES A PRO-INFLAMMATORY EXPRESSION PROFILE IN ADIPOCYTES 49 4.2.1 TRAIL INDUCES A PRO-INFLAMMATORY EXPRESSION PROFILE IN PREADIPOCYTES AND ADIPOCYTES 49 4.2.2 TRAIL INDUCES THE PRODUCTION OF IL-6, IL-8, MCP-1 AND CCL-20 IN A TIME- AND DOSE-DEPENDENT MANNERSO 4.2.3 TRAIL INDUCES IL-6, IL-8 AND CCL-20 EXPRESSION IN HUMAN PRIMARY ADIPOCYTES 52 4.2.4 CASPASES ARE NOT INVOLVED IN MEDIATING THE EFFECT OF TRAIL ON CYTOKINE EXPRESSION 53 4.2.5 TRAIL INDUCES PHOSPHORYLATION OF IXBA 54 4.2.6 TRAIL INDUCES A PHOSPHORYLATION OF ERK1/2 58 5. DISCUSSION 61 5.1 USED TRAIL CONCENTRATIONS IN THIS STUDY 61 5.2 TRAIL INHIBITS ADIPOGENIC DIFFERENTIATION 61 5.2.2 MOLECULAR MECHANISM OF TRAIL IN REDUCING ADIPOGENIC DIFFERENTIATION 62 5.2.3 NON-APOPTOTIC FUNCTIONS OF CASPASES 63 5.2.4 TRAIL INHIBITS IMPORTANT ADIPOGENIC TRANSCRIPTION FACTORS 64 5.3.1 MOLECULAR MECHANISM OF TRAIL IN MEDIATING AN INFLAMMATORY SECRETION PROFILE 66 5.4 IMPLICATIONS OF THE STUDY 68 6. SUMMARY 73 REFERENCES 75 STATUTORY DECLARATION 98 ACKNOWLEDGEMENTS 99 CURRICULUM VITAE 100
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spelling	Zoller, Verena 1987- Verfasser (DE-588)1160882703 aut Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller Ulm [2018] 101 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2018 Fettsucht (DE-588)4016953-4 gnd rswk-swf Obesity. TNF-related apoptosis-inducing ligand. (DE-588)4113937-9 Hochschulschrift gnd-content Fettsucht (DE-588)4016953-4 s DE-604 Universität Ulm (DE-588)30607-1 dgg B:DE-101 application/pdf http://d-nb.info/1161872604/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612786&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Zoller, Verena 1987- Regulation of adipose tissue homeostasis by the death ligand TRAIL Fettsucht (DE-588)4016953-4 gnd
subject_GND	(DE-588)4016953-4 (DE-588)4113937-9
title	Regulation of adipose tissue homeostasis by the death ligand TRAIL
title_auth	Regulation of adipose tissue homeostasis by the death ligand TRAIL
title_exact_search	Regulation of adipose tissue homeostasis by the death ligand TRAIL
title_full	Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller
title_fullStr	Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller
title_full_unstemmed	Regulation of adipose tissue homeostasis by the death ligand TRAIL Verena Zoller
title_short	Regulation of adipose tissue homeostasis by the death ligand TRAIL
title_sort	regulation of adipose tissue homeostasis by the death ligand trail
topic	Fettsucht (DE-588)4016953-4 gnd
topic_facet	Fettsucht Hochschulschrift
url	http://d-nb.info/1161872604/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030612786&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT zollerverena regulationofadiposetissuehomeostasisbythedeathligandtrail AT universitatulm regulationofadiposetissuehomeostasisbythedeathligandtrail

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