Bacterial Protein Secretion Systems: Methods and Protocols
This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complex...
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| Other Authors: | , |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2017
|
| Series: | Methods in Molecular Biology
1615 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope |
| Physical Description: | 1 Online-Ressource (XV, 528 p. 96 illus., 70 illus. in color) |
| ISBN: | 9781493970339 |
| DOI: | 10.1007/978-1-4939-7033-9 |
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| indexdate | 2024-07-10T08:05:37Z |
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| isbn | 9781493970339 |
| language | English |
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| spelling | Bacterial Protein Secretion Systems Methods and Protocols edited by Laure Journet, Eric Cascales New York, NY Springer New York 2017 1 Online-Ressource (XV, 528 p. 96 illus., 70 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1615 This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope Life Sciences Microbiology Life sciences Journet, Laure edt Cascales, Eric edt Erscheint auch als Druck-Ausgabe 9781493970315 https://doi.org/10.1007/978-1-4939-7033-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Bacterial Protein Secretion Systems Methods and Protocols Life Sciences Microbiology Life sciences |
| title | Bacterial Protein Secretion Systems Methods and Protocols |
| title_auth | Bacterial Protein Secretion Systems Methods and Protocols |
| title_exact_search | Bacterial Protein Secretion Systems Methods and Protocols |
| title_full | Bacterial Protein Secretion Systems Methods and Protocols edited by Laure Journet, Eric Cascales |
| title_fullStr | Bacterial Protein Secretion Systems Methods and Protocols edited by Laure Journet, Eric Cascales |
| title_full_unstemmed | Bacterial Protein Secretion Systems Methods and Protocols edited by Laure Journet, Eric Cascales |
| title_short | Bacterial Protein Secretion Systems |
| title_sort | bacterial protein secretion systems methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Microbiology Life sciences |
| topic_facet | Life Sciences Microbiology Life sciences |
| url | https://doi.org/10.1007/978-1-4939-7033-9 |
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