Heterologous Gene Expression in E.coli: Methods and Protocols
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular pro...
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| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
705 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host |
| Beschreibung: | 1 Online-Ressource (XI, 310 p) |
| ISBN: | 9781617379673 |
| DOI: | 10.1007/978-1-61737-967-3 |
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Datensatz im Suchindex
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| dewey-full | 611.01816 599.935 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia |
| dewey-raw | 611.01816 599.935 |
| dewey-search | 611.01816 599.935 |
| dewey-sort | 3611.01816 |
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| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-61737-967-3 |
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| spelling | Heterologous Gene Expression in E.coli Methods and Protocols edited by Thomas C. Evans, Jr., Ming-Qun Xu Totowa, NJ Humana Press 2011 1 Online-Ressource (XI, 310 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 705 Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host Biomedicine Human Genetics Proteomics Medicine Human genetics Escherichia coli (DE-588)4070959-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Heterologe Genexpression (DE-588)4342015-1 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Escherichia coli (DE-588)4070959-0 s Heterologe Genexpression (DE-588)4342015-1 s Methode (DE-588)4038971-6 s 2\p DE-604 Evans, Jr., Thomas C. edt Xu, Ming-Qun edt Erscheint auch als Druck-Ausgabe 9781617379666 https://doi.org/10.1007/978-1-61737-967-3 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Heterologous Gene Expression in E.coli Methods and Protocols Biomedicine Human Genetics Proteomics Medicine Human genetics Escherichia coli (DE-588)4070959-0 gnd Methode (DE-588)4038971-6 gnd Heterologe Genexpression (DE-588)4342015-1 gnd |
| subject_GND | (DE-588)4070959-0 (DE-588)4038971-6 (DE-588)4342015-1 (DE-588)4143413-4 |
| title | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_auth | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_exact_search | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_full | Heterologous Gene Expression in E.coli Methods and Protocols edited by Thomas C. Evans, Jr., Ming-Qun Xu |
| title_fullStr | Heterologous Gene Expression in E.coli Methods and Protocols edited by Thomas C. Evans, Jr., Ming-Qun Xu |
| title_full_unstemmed | Heterologous Gene Expression in E.coli Methods and Protocols edited by Thomas C. Evans, Jr., Ming-Qun Xu |
| title_short | Heterologous Gene Expression in E.coli |
| title_sort | heterologous gene expression in e coli methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Proteomics Medicine Human genetics Escherichia coli (DE-588)4070959-0 gnd Methode (DE-588)4038971-6 gnd Heterologe Genexpression (DE-588)4342015-1 gnd |
| topic_facet | Biomedicine Human Genetics Proteomics Medicine Human genetics Escherichia coli Methode Heterologe Genexpression Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-61737-967-3 |
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