Plant membrane proteomics: methods and protocols

This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from N...

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Bibliographic Details
Other Authors: Mock, Hans-Peter (Editor), Matros, Andrea (Editor), Witzel, Katja 1978- (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY Springer New York 2018
Series:Methods in Molecular Biology 1696
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Online Access:UBR01
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Summary:This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field
Physical Description:1 Online-Ressource (XII, 280 Seiten) Illustrationen
ISBN:9781493974115
DOI:10.1007/978-1-4939-7411-5

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